Restriction map for
pFN21AE6293
(Linear) (Six-base) MAPSORT of: KIEE6293.seq Check: 8655 from: 1 to: 732
KIEE6293.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 406 732
Size: 406 326
AgeI A'CCGG_T
Cuts at: 0 172 732
Size: 172 560
ApaI G_GGCC'C
Cuts at: 0 712 732
Size: 712 20
ApoI r'AATT_y
Cuts at: 0 178 283 732
Size: 178 105 449
Fragments arranged by size:
449 178 105
AvaI C'yCGr_G
Cuts at: 0 484 587 721 732
Size: 484 103 134 11
Fragments arranged by size:
484 134 103 11
BamHI G'GATC_C
Cuts at: 0 253 732
Size: 253 479
BanI G'GyrC_C
Cuts at: 0 492 732
Size: 492 240
BanII G_rGCy'C
Cuts at: 0 483 712 732
Size: 483 229 20
Fragments arranged by size:
483 229 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 194 535 732
Size: 194 341 197
Fragments arranged by size:
341 197 194
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 342 376 732
Size: 342 34 356
Fragments arranged by size:
356 342 34
BglII A'GATC_T
Cuts at: 0 533 732
Size: 533 199
Bme1580I G_kGCm'C
Cuts at: 0 497 712 732
Size: 497 215 20
Fragments arranged by size:
497 215 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 683 732
Size: 683 49
BsaWI w'CCGG_w
Cuts at: 0 172 732
Size: 172 560
BseYI C'CCAG_C
Cuts at: 0 123 497 732
Size: 123 374 235
Fragments arranged by size:
374 235 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 732
Size: 434 298
BsiHKAI G_wGCw'C
Cuts at: 0 596 732
Size: 596 136
Bsp1286I G_dGCh'C
Cuts at: 0 483 497 596 712 732
Size: 483 14 99 116 20
Fragments arranged by size:
483 116 99 20 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 406 479 732
Size: 406 73 253
Fragments arranged by size:
406 253 73
BsrFI r'CCGG_y
Cuts at: 0 172 732
Size: 172 560
BstXI CCAn_nnnn'nTGG
Cuts at: 0 399 538 732
Size: 399 139 194
Fragments arranged by size:
399 194 139
BstYI r'GATC_y
Cuts at: 0 253 533 732
Size: 253 280 199
Fragments arranged by size:
280 253 199
BtsI GCAGTG_nn'
Cuts at: 0 127 732
Size: 127 605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 354 389 600 635 732
Size: 354 35 211 35 97
Fragments arranged by size:
354 211 97 35 35
EarI CTCTTCn'nnn_
Cuts at: 0 508 686 732
Size: 508 178 46
Fragments arranged by size:
508 178 46
EcoO109I rG'GnC_Cy
Cuts at: 0 299 708 732
Size: 299 409 24
Fragments arranged by size:
409 299 24
EcoRI G'AATT_C
Cuts at: 0 178 732
Size: 178 554
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 233 265 525 557 732
Size: 233 32 260 32 175
Fragments arranged by size:
260 233 175 32 32
HincII GTy'rAC
Cuts at: 0 117 732
Size: 117 615
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 732
Size: 452 280
MslI CAynn'nnrTG
Cuts at: 0 523 732
Size: 523 209
PspOMI G'GGCC_C
Cuts at: 0 708 732
Size: 708 24
PstI C_TGCA'G
Cuts at: 0 672 732
Size: 672 60
SapI GCTCTTCn'nnn_
Cuts at: 0 508 732
Size: 508 224
SfcI C'TryA_G
Cuts at: 0 668 732
Size: 668 64
StyI C'CwwG_G
Cuts at: 0 272 618 701 732
Size: 272 346 83 31
Fragments arranged by size:
346 272 83 31
TatI w'GTAC_w
Cuts at: 0 443 732
Size: 443 289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 399 732
Size: 399 333
Enzymes that do cut:
AarI AgeI ApaI ApoI AvaI BamHI BanI BanII
BbsI BcgI BglII Bme1580I BsaI BsaWI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrFI BstXI BstYI BtsI CspCI
EarI EcoO109I EcoRI Hin4I HincII MmeI MslI PspOMI
PstI SapI SfcI StyI TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BbvCI BciVI BclI BfrBI
BglI BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
ClaI DraI DraIII DrdI EaeI EagI EciI EcoICRI
Eco57MI EcoNI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MscI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI XmnI ZraI