Restriction map for
pFN21AE6291
(Linear) (Six-base) MAPSORT of: KIEE6291.seq Check: 1618 from: 1 to: 894
KIEE6291.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AccI GT'mk_AC
Cuts at: 0 764 894
Size: 764 130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 768 894
Size: 513 255 126
Fragments arranged by size:
513 255 126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 733 894
Size: 733 161
BanI G'GyrC_C
Cuts at: 0 757 850 894
Size: 757 93 44
Fragments arranged by size:
757 93 44
BanII G_rGCy'C
Cuts at: 0 881 894
Size: 881 13
BbeI G_GCGC'C
Cuts at: 0 854 894
Size: 854 40
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 114 148 894
Size: 114 34 746
Fragments arranged by size:
746 114 34
BglII A'GATC_T
Cuts at: 0 290 779 894
Size: 290 489 115
Fragments arranged by size:
489 290 115
Bme1580I G_kGCm'C
Cuts at: 0 760 894
Size: 760 134
BmgBI CAC'GTC
Cuts at: 0 432 894
Size: 432 462
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 348 894
Size: 231 117 546
Fragments arranged by size:
546 231 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 865 894
Size: 236 629 29
Fragments arranged by size:
629 236 29
BsaHI Gr'CG_yC
Cuts at: 0 851 894
Size: 851 43
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 337 367 894
Size: 337 30 527
Fragments arranged by size:
527 337 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 894
Size: 562 332
BsiEI CG_ry'CG
Cuts at: 0 74 86 368 665 894
Size: 74 12 282 297 229
Fragments arranged by size:
297 282 229 74 12
BsiHKAI G_wGCw'C
Cuts at: 0 881 894
Size: 881 13
Bsp1286I G_dGCh'C
Cuts at: 0 760 881 894
Size: 760 121 13
Fragments arranged by size:
760 121 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 99 894
Size: 99 795
BsrBI CCG'CTC
Cuts at: 0 90 894
Size: 90 804
BsrFI r'CCGG_y
Cuts at: 0 367 670 894
Size: 367 303 224
Fragments arranged by size:
367 303 224
BsrGI T'GTAC_A
Cuts at: 0 749 894
Size: 749 145
BstAPI GCAn_nnn'nTGC
Cuts at: 0 198 894
Size: 198 696
BstYI r'GATC_y
Cuts at: 0 290 779 894
Size: 290 489 115
Fragments arranged by size:
489 290 115
BtgI C'CryG_G
Cuts at: 0 74 665 894
Size: 74 591 229
Fragments arranged by size:
591 229 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 33 894
Size: 33 861
DraIII CAC_nnn'GTG
Cuts at: 0 630 894
Size: 630 264
DrdI GACnn_nn'nnGTC
Cuts at: 0 172 894
Size: 172 722
EaeI y'GGCC_r
Cuts at: 0 38 71 365 590 662 668 894
Size: 38 33 294 225 72 6 226
Fragments arranged by size:
294 226 225 72 38 33 6
EagI C'GGCC_G
Cuts at: 0 71 365 662 894
Size: 71 294 297 232
Fragments arranged by size:
297 294 232 71
EcoICRI GAG'CTC
Cuts at: 0 879 894
Size: 879 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 348 513 768 894
Size: 231 117 165 255 126
Fragments arranged by size:
255 231 165 126 117
EcoNI CCTnn'n_nnAGG
Cuts at: 0 210 520 894
Size: 210 310 374
Fragments arranged by size:
374 310 210
EcoO109I rG'GnC_Cy
Cuts at: 0 513 894
Size: 513 381
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 309 825 894
Size: 309 516 69
Fragments arranged by size:
516 309 69
HaeII r_GCGC'y
Cuts at: 0 854 894
Size: 854 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 728 760 894
Size: 728 32 134
Fragments arranged by size:
728 134 32
HincII GTy'rAC
Cuts at: 0 765 894
Size: 765 129
KasI G'GCGC_C
Cuts at: 0 850 894
Size: 850 44
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 751 851 894
Size: 524 227 100 43
Fragments arranged by size:
524 227 100 43
MslI CAynn'nnrTG
Cuts at: 0 631 715 894
Size: 631 84 179
Fragments arranged by size:
631 179 84
MspA1I CmG'CkG
Cuts at: 0 399 427 894
Size: 399 28 467
Fragments arranged by size:
467 399 28
NaeI GCC'GGC
Cuts at: 0 369 672 894
Size: 369 303 222
Fragments arranged by size:
369 303 222
NarI GG'CG_CC
Cuts at: 0 851 894
Size: 851 43
NgoMIV G'CCGG_C
Cuts at: 0 367 670 894
Size: 367 303 224
Fragments arranged by size:
367 303 224
NspI r_CATG'y
Cuts at: 0 603 894
Size: 603 291
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 442 474 894
Size: 442 32 420
Fragments arranged by size:
442 420 32
PstI C_TGCA'G
Cuts at: 0 110 894
Size: 110 784
PvuI CG_AT'CG
Cuts at: 0 86 894
Size: 86 808
SacI G_AGCT'C
Cuts at: 0 881 894
Size: 881 13
SalI G'TCGA_C
Cuts at: 0 763 894
Size: 763 131
SfcI C'TryA_G
Cuts at: 0 106 894
Size: 106 788
SfoI GGC'GCC
Cuts at: 0 852 894
Size: 852 42
SmlI C'TyrA_G
Cuts at: 0 251 880 894
Size: 251 629 14
Fragments arranged by size:
629 251 14
SphI G_CATG'C
Cuts at: 0 603 894
Size: 603 291
StuI AGG'CCT
Cuts at: 0 467 805 894
Size: 467 338 89
Fragments arranged by size:
467 338 89
StyI C'CwwG_G
Cuts at: 0 185 593 894
Size: 185 408 301
Fragments arranged by size:
408 301 185
TatI w'GTAC_w
Cuts at: 0 149 281 749 894
Size: 149 132 468 145
Fragments arranged by size:
468 149 145 132
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 33 72 417 894
Size: 33 39 345 477
Fragments arranged by size:
477 345 39 33
XmnI GAAnn'nnTTC
Cuts at: 0 781 894
Size: 781 113
Enzymes that do cut:
AccI AcuI AlfI BanI BanII BbeI BcgI BglII
Bme1580I BmgBI BpmI BpuEI BsaHI BsaXI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BstAPI BstYI
BtgI BtgZI DraIII DrdI EaeI EagI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII KasI MmeI
MslI MspA1I NaeI NarI NgoMIV NspI PpiI PstI
PvuI SacI SalI SfcI SfoI SmlI SphI StuI
StyI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbsI BbvCI
BciVI BclI BfrBI BglI BlpI BmrI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaWI BseRI BseYI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI
EarI EciI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MscI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XhoI XmaI ZraI