Restriction map for
pFN21AE6288
(Linear) (Six-base) MAPSORT of: KIEE6288.seq Check: 8327 from: 1 to: 921
KIEE6288.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 431 545 687 921
Size: 431 114 142 234
Fragments arranged by size:
431 234 142 114
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 921
Size: 358 563
AflIII A'CryG_T
Cuts at: 0 14 921
Size: 14 907
AleI CACnn'nnGTG
Cuts at: 0 472 921
Size: 472 449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 490 921
Size: 490 431
AlwNI CAG_nnn'CTG
Cuts at: 0 357 921
Size: 357 564
ApaI G_GGCC'C
Cuts at: 0 185 569 614 715 921
Size: 185 384 45 101 206
Fragments arranged by size:
384 206 185 101 45
AvaI C'yCGr_G
Cuts at: 0 113 139 178 669 818 921
Size: 113 26 39 491 149 103
Fragments arranged by size:
491 149 113 103 39 26
BanI G'GyrC_C
Cuts at: 0 94 921
Size: 94 827
BanII G_rGCy'C
Cuts at: 0 179 185 410 569 614 715 921
Size: 179 6 225 159 45 101 206
Fragments arranged by size:
225 206 179 159 101 45 6
BbeI G_GCGC'C
Cuts at: 0 98 921
Size: 98 823
BbsI GAAGACnn'nnnn_
Cuts at: 0 99 921
Size: 99 822
BglI GCCn_nnn'nGGC
Cuts at: 0 67 76 118 604 921
Size: 67 9 42 486 317
Fragments arranged by size:
486 317 67 42 9
Bme1580I G_kGCm'C
Cuts at: 0 185 479 569 614 715 921
Size: 185 294 90 45 101 206
Fragments arranged by size:
294 206 185 101 90 45
BmgBI CAC'GTC
Cuts at: 0 620 921
Size: 620 301
BmrI ACTGGGnnnn_n'
Cuts at: 0 82 921
Size: 82 839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 706 921
Size: 175 531 215
Fragments arranged by size:
531 215 175
BsaAI yAC'GTr
Cuts at: 0 693 921
Size: 693 228
BsaHI Gr'CG_yC
Cuts at: 0 95 921
Size: 95 826
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 921
Size: 328 593
BseYI C'CCAG_C
Cuts at: 0 190 357 395 708 921
Size: 190 167 38 313 213
Fragments arranged by size:
313 213 190 167 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 360 403 517 715 730 921
Size: 154 206 43 114 198 15 191
Fragments arranged by size:
206 198 191 154 114 43 15
BsiEI CG_ry'CG
Cuts at: 0 608 921
Size: 608 313
BsiHKAI G_wGCw'C
Cuts at: 0 179 410 816 921
Size: 179 231 406 105
Fragments arranged by size:
406 231 179 105
Bsp1286I G_dGCh'C
Cuts at: 0 179 185 410 479 569 614 715 816
Size: 179 6 225 69 90 45 101 101
Cuts at: 816 921
Size: 105
Fragments arranged by size:
225 179 105 101 101 90 69 45
6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 218 425 431 545 687 921
Size: 218 207 6 114 142 234
Fragments arranged by size:
234 218 207 142 114 6
BsrFI r'CCGG_y
Cuts at: 0 828 921
Size: 828 93
BstAPI GCAn_nnn'nTGC
Cuts at: 0 426 921
Size: 426 495
BstEII G'GTnAC_C
Cuts at: 0 252 585 921
Size: 252 333 336
Fragments arranged by size:
336 333 252
Bsu36I CC'TnA_GG
Cuts at: 0 840 921
Size: 840 81
BtgI C'CryG_G
Cuts at: 0 480 921
Size: 480 441
BtsI GCAGTG_nn'
Cuts at: 0 900 921
Size: 900 21
EaeI y'GGCC_r
Cuts at: 0 272 308 605 644 792 921
Size: 272 36 297 39 148 129
Fragments arranged by size:
297 272 148 129 39 36
EagI C'GGCC_G
Cuts at: 0 605 921
Size: 605 316
EarI CTCTTCn'nnn_
Cuts at: 0 232 241 921
Size: 232 9 680
Fragments arranged by size:
680 232 9
EcoICRI GAG'CTC
Cuts at: 0 177 408 921
Size: 177 231 513
Fragments arranged by size:
513 231 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 358 706 921
Size: 175 183 348 215
Fragments arranged by size:
348 215 183 175
EcoNI CCTnn'n_nnAGG
Cuts at: 0 52 719 921
Size: 52 667 202
Fragments arranged by size:
667 202 52
EcoO109I rG'GnC_Cy
Cuts at: 0 78 143 182 266 611 712 921
Size: 78 65 39 84 345 101 209
Fragments arranged by size:
345 209 101 84 78 65 39
FspI TGC'GCA
Cuts at: 0 466 921
Size: 466 455
HaeII r_GCGC'y
Cuts at: 0 98 490 921
Size: 98 392 431
Fragments arranged by size:
431 392 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 253 285 921
Size: 253 32 636
Fragments arranged by size:
636 253 32
KasI G'GCGC_C
Cuts at: 0 94 921
Size: 94 827
MslI CAynn'nnrTG
Cuts at: 0 472 921
Size: 472 449
MspA1I CmG'CkG
Cuts at: 0 563 571 921
Size: 563 8 350
Fragments arranged by size:
563 350 8
NaeI GCC'GGC
Cuts at: 0 830 921
Size: 830 91
NarI GG'CG_CC
Cuts at: 0 95 921
Size: 95 826
NgoMIV G'CCGG_C
Cuts at: 0 828 921
Size: 828 93
NspI r_CATG'y
Cuts at: 0 366 921
Size: 366 555
PasI CC'CwG_GG
Cuts at: 0 185 921
Size: 185 736
PfoI T'CCnGG_A
Cuts at: 0 152 878 921
Size: 152 726 43
Fragments arranged by size:
726 152 43
PshAI GACnn'nnGTC
Cuts at: 0 169 921
Size: 169 752
PspOMI G'GGCC_C
Cuts at: 0 181 565 610 711 921
Size: 181 384 45 101 210
Fragments arranged by size:
384 210 181 101 45
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 772 804 921
Size: 772 32 117
Fragments arranged by size:
772 117 32
PstI C_TGCA'G
Cuts at: 0 296 431 454 921
Size: 296 135 23 467
Fragments arranged by size:
467 296 135 23
SacI G_AGCT'C
Cuts at: 0 179 410 921
Size: 179 231 511
Fragments arranged by size:
511 231 179
SapI GCTCTTCn'nnn_
Cuts at: 0 232 241 921
Size: 232 9 680
Fragments arranged by size:
680 232 9
SbfI CC_TGCA'GG
Cuts at: 0 296 921
Size: 296 625
SfcI C'TryA_G
Cuts at: 0 292 427 450 558 921
Size: 292 135 23 108 363
Fragments arranged by size:
363 292 135 108 23
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 76 604 921
Size: 76 528 317
Fragments arranged by size:
528 317 76
SfoI GGC'GCC
Cuts at: 0 96 921
Size: 96 825
SmaI CCC'GGG
Cuts at: 0 115 921
Size: 115 806
SphI G_CATG'C
Cuts at: 0 366 921
Size: 366 555
StyI C'CwwG_G
Cuts at: 0 74 257 921
Size: 74 183 664
Fragments arranged by size:
664 183 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 865 921
Size: 865 56
TatI w'GTAC_w
Cuts at: 0 701 921
Size: 701 220
XmaI C'CCGG_G
Cuts at: 0 113 921
Size: 113 808
XmnI GAAnn'nnTTC
Cuts at: 0 784 921
Size: 784 137
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaI AvaI
BanI BanII BbeI BbsI BglI Bme1580I BmgBI BmrI
BpmI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrFI BstAPI BstEII Bsu36I BtgI BtsI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I KasI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PfoI PshAI PspOMI PsrI PstI SacI
SapI SbfI SfcI SfiI SfoI SmaI SphI StyI
TaqII TatI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstBI BstXI BstYI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EciI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PflMI PmeI PmlI PpiI PpuMI PsiI
PspXI PvuI PvuII RsrII SacII SalI SanDI ScaI
SexAI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI XhoI ZraI