(Linear) (Six-base) MAPSORT of: KIEE6285.seq  Check: 1124  from: 1  to: 837



KIEE6285.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:29  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     569     837 
   Size:        169     400     268

  Fragments arranged by size: 

                 400     268     169
 
AflII C'TTAA_G

Cuts at:      0     328     837 
   Size:        328     509
 
AlwNI CAG_nnn'CTG

Cuts at:      0      36     837 
   Size:         36     801
 
ApoI r'AATT_y

Cuts at:      0      79     837 
   Size:         79     758
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     329     837 
   Size:        329     508
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     734     837 
   Size:        734     103
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     479     837 
   Size:        479     358
 
BsaAI yAC'GTr

Cuts at:      0     832     837 
   Size:        832       5
 
BsaWI w'CCGG_w

Cuts at:      0      73     837 
   Size:         73     764
 
BseYI C'CCAG_C

Cuts at:      0     104     837 
   Size:        104     733
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     837 
   Size:        137     700
 
BsiHKAI G_wGCw'C

Cuts at:      0     396     837 
   Size:        396     441
 
Bsp1286I G_dGCh'C

Cuts at:      0     396     837 
   Size:        396     441
 
BtsI GCAGTG_nn'

Cuts at:      0     468     837 
   Size:        468     369
 
DraIII CAC_nnn'GTG

Cuts at:      0     173     837 
   Size:        173     664
 
EaeI y'GGCC_r

Cuts at:      0      38     837 
   Size:         38     799
 
EarI CTCTTCn'nnn_

Cuts at:      0      58     127     163     837 
   Size:         58      69      36     674

  Fragments arranged by size: 

                 674      69      58      36
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     569     837 
   Size:        169     400     268

  Fragments arranged by size: 

                 400     268     169
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     233     837 
   Size:        233     604
 
EcoRI G'AATT_C

Cuts at:      0      79     837 
   Size:         79     758
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       4      36     837 
   Size:          4      32     801

  Fragments arranged by size: 

                 801      32       4
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     837 
   Size:        322     515
 
NspI r_CATG'y

Cuts at:      0     431     461     670     837 
   Size:        431      30     209     167

  Fragments arranged by size: 

                 431     209     167      30
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      36     104     652     837 
   Size:         36      68     548     185

  Fragments arranged by size: 

                 548     185      68      36
 
PmlI CAC'GTG

Cuts at:      0     832     837 
   Size:        832       5
 
SapI GCTCTTCn'nnn_

Cuts at:      0      58     127     837 
   Size:         58      69     710

  Fragments arranged by size: 

                 710      69      58
 
SfcI C'TryA_G

Cuts at:      0     679     837 
   Size:        679     158
 
SmlI C'TyrA_G

Cuts at:      0     328     494     837 
   Size:        328     166     343

  Fragments arranged by size: 

                 343     328     166
 
SphI G_CATG'C

Cuts at:      0     461     837 
   Size:        461     376
 
SspI AAT'ATT

Cuts at:      0     640     837 
   Size:        640     197
 
StyI C'CwwG_G

Cuts at:      0     559     837 
   Size:        559     278
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     366     398     555     587     837 
   Size:        366      32     157      32     250

  Fragments arranged by size: 

                 366     250     157      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0      52     837 
   Size:         52     785
 

 Enzymes that do cut:

     AcuI    AflII    AlwNI     ApoI     BbsI     BmrI    BpuEI    BsaAI
    BsaWI    BseYI     BsgI  BsiHKAI Bsp1286I     BtsI   DraIII     EaeI
     EarI  Eco57MI    EcoNI    EcoRI    Hin4I     MmeI     NspI    PflMI
     PmlI     SapI     SfcI     SmlI     SphI     SspI     StyI     TstI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaBI
    BsaHI    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI     DrdI     EagI     EciI  EcoICRI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI