Restriction map for
pFN21AE6283
(Linear) (Six-base) MAPSORT of: KIEE6283.seq Check: 8066 from: 1 to: 804
KIEE6283.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
Acc65I G'GTAC_C
Cuts at: 0 379 804
Size: 379 425
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 524 804
Size: 317 207 280
Fragments arranged by size:
317 280 207
AlwNI CAG_nnn'CTG
Cuts at: 0 328 804
Size: 328 476
AvaI C'yCGr_G
Cuts at: 0 65 804
Size: 65 739
BanI G'GyrC_C
Cuts at: 0 345 379 631 804
Size: 345 34 252 173
Fragments arranged by size:
345 252 173 34
BanII G_rGCy'C
Cuts at: 0 7 130 502 804
Size: 7 123 372 302
Fragments arranged by size:
372 302 123 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 241 305 804
Size: 241 64 499
Fragments arranged by size:
499 241 64
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 46 80 804
Size: 46 34 724
Fragments arranged by size:
724 46 34
BglII A'GATC_T
Cuts at: 0 535 804
Size: 535 269
BlpI GC'TnA_GC
Cuts at: 0 6 804
Size: 6 798
BmrI ACTGGGnnnn_n'
Cuts at: 0 16 378 804
Size: 16 362 426
Fragments arranged by size:
426 362 16
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 450 804
Size: 450 354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 755 804
Size: 184 571 49
Fragments arranged by size:
571 184 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 498 804
Size: 498 306
BsaI GGTCTCn'nnnn_
Cuts at: 0 242 804
Size: 242 562
BsiHKAI G_wGCw'C
Cuts at: 0 130 502 804
Size: 130 372 302
Fragments arranged by size:
372 302 130
Bsp1286I G_dGCh'C
Cuts at: 0 7 130 502 804
Size: 7 123 372 302
Fragments arranged by size:
372 302 123 7
BsrBI CCG'CTC
Cuts at: 0 200 804
Size: 200 604
BstXI CCAn_nnnn'nTGG
Cuts at: 0 120 804
Size: 120 684
BstYI r'GATC_y
Cuts at: 0 192 535 592 804
Size: 192 343 57 212
Fragments arranged by size:
343 212 192 57
BtsI GCAGTG_nn'
Cuts at: 0 249 804
Size: 249 555
DraIII CAC_nnn'GTG
Cuts at: 0 91 804
Size: 91 713
EaeI y'GGCC_r
Cuts at: 0 488 804
Size: 488 316
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 32 88 804
Size: 32 56 716
Fragments arranged by size:
716 56 32
EcoICRI GAG'CTC
Cuts at: 0 128 500 804
Size: 128 372 304
Fragments arranged by size:
372 304 128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 317 524 755 804
Size: 184 133 207 231 49
Fragments arranged by size:
231 207 184 133 49
EcoO109I rG'GnC_Cy
Cuts at: 0 519 804
Size: 519 285
FspI TGC'GCA
Cuts at: 0 106 553 804
Size: 106 447 251
Fragments arranged by size:
447 251 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 184 216 292 324 496 528 584 607
Size: 184 32 76 32 172 32 56 23
Cuts at: 607 616 639 804
Size: 9 23 165
Fragments arranged by size:
184 172 165 76 56 32 32 32
23 23 9
KpnI G_GTAC'C
Cuts at: 0 383 804
Size: 383 421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 804
Size: 527 277
MscI TGG'CCA
Cuts at: 0 490 804
Size: 490 314
MslI CAynn'nnrTG
Cuts at: 0 118 804
Size: 118 686
MspA1I CmG'CkG
Cuts at: 0 276 804
Size: 276 528
NspI r_CATG'y
Cuts at: 0 181 804
Size: 181 623
PpuMI rG'GwC_Cy
Cuts at: 0 519 804
Size: 519 285
PspXI vC'TCGA_Gb
Cuts at: 0 65 804
Size: 65 739
PstI C_TGCA'G
Cuts at: 0 614 804
Size: 614 190
PvuII CAG'CTG
Cuts at: 0 276 804
Size: 276 528
SacI G_AGCT'C
Cuts at: 0 130 502 804
Size: 130 372 302
Fragments arranged by size:
372 302 130
SanDI GG'GwC_CC
Cuts at: 0 519 804
Size: 519 285
SbfI CC_TGCA'GG
Cuts at: 0 614 804
Size: 614 190
SfcI C'TryA_G
Cuts at: 0 610 804
Size: 610 194
SmlI C'TyrA_G
Cuts at: 0 65 513 804
Size: 65 448 291
Fragments arranged by size:
448 291 65
StuI AGG'CCT
Cuts at: 0 143 804
Size: 143 661
StyI C'CwwG_G
Cuts at: 0 412 452 791 804
Size: 412 40 339 13
Fragments arranged by size:
412 339 40 13
XhoI C'TCGA_G
Cuts at: 0 65 804
Size: 65 739
Enzymes that do cut:
Acc65I AcuI AlwNI AvaI BanI BanII BbsI BcgI
BglII BlpI BmrI BplI BpmI BpuEI BsaI BsiHKAI
Bsp1286I BsrBI BstXI BstYI BtsI DraIII EaeI EciI
EcoICRI Eco57MI EcoO109I FspI Hin4I KpnI MmeI MscI
MslI MspA1I NspI PpuMI PspXI PstI PvuII SacI
SanDI SbfI SfcI SmlI StuI StyI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI
BclI BfrBI BglI Bme1580I BmgBI BmtI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DrdI EagI EarI
EcoNI EcoRI EcoRV FalI FseI FspAI HaeII HincII
HindIII HpaI KasI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PsrI PvuI RsrII SacII SalI SapI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI