Restriction map for
pFN21AE6282
(Linear) (Six-base) MAPSORT of: KIEE6282.seq Check: 5865 from: 1 to: 768
KIEE6282.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 768
Size: 70 698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 410 768
Size: 410 358
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 143 175 768
Size: 143 32 593
Fragments arranged by size:
593 143 32
AlwNI CAG_nnn'CTG
Cuts at: 0 474 768
Size: 474 294
ApoI r'AATT_y
Cuts at: 0 184 768
Size: 184 584
AvaI C'yCGr_G
Cuts at: 0 8 768
Size: 8 760
AvrII C'CTAG_G
Cuts at: 0 66 768
Size: 66 702
BglI GCCn_nnn'nGGC
Cuts at: 0 37 768
Size: 37 731
BmrI ACTGGGnnnn_n'
Cuts at: 0 251 768
Size: 251 517
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 734 768
Size: 734 34
BsaI GGTCTCn'nnnn_
Cuts at: 0 286 768
Size: 286 482
BsaAI yAC'GTr
Cuts at: 0 666 768
Size: 666 102
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 143 173 768
Size: 143 30 595
Fragments arranged by size:
595 143 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 768
Size: 628 140
BsmI GAATG_Cn'
Cuts at: 0 60 447 768
Size: 60 387 321
Fragments arranged by size:
387 321 60
BsrDI GCAATG_nn'
Cuts at: 0 518 716 768
Size: 518 198 52
Fragments arranged by size:
518 198 52
BstAPI GCAn_nnn'nTGC
Cuts at: 0 367 669 768
Size: 367 302 99
Fragments arranged by size:
367 302 99
Bsu36I CC'TnA_GG
Cuts at: 0 179 768
Size: 179 589
BtsI GCAGTG_nn'
Cuts at: 0 299 768
Size: 299 469
DraIII CAC_nnn'GTG
Cuts at: 0 669 768
Size: 669 99
EarI CTCTTCn'nnn_
Cuts at: 0 143 401 768
Size: 143 258 367
Fragments arranged by size:
367 258 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 12 768
Size: 12 756
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 768
Size: 70 698
EcoO109I rG'GnC_Cy
Cuts at: 0 95 223 768
Size: 95 128 545
Fragments arranged by size:
545 128 95
EcoRI G'AATT_C
Cuts at: 0 184 768
Size: 184 584
HaeII r_GCGC'y
Cuts at: 0 489 768
Size: 489 279
MfeI C'AATT_G
Cuts at: 0 446 704 768
Size: 446 258 64
Fragments arranged by size:
446 258 64
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 768
Size: 453 315
MslI CAynn'nnrTG
Cuts at: 0 523 768
Size: 523 245
MspA1I CmG'CkG
Cuts at: 0 37 768
Size: 37 731
PmlI CAC'GTG
Cuts at: 0 666 768
Size: 666 102
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 143 175 768
Size: 143 32 593
Fragments arranged by size:
593 143 32
PpuMI rG'GwC_Cy
Cuts at: 0 95 223 768
Size: 95 128 545
Fragments arranged by size:
545 128 95
ScaI AGT'ACT
Cuts at: 0 314 768
Size: 314 454
SmaI CCC'GGG
Cuts at: 0 10 768
Size: 10 758
SmlI C'TyrA_G
Cuts at: 0 749 768
Size: 749 19
StyI C'CwwG_G
Cuts at: 0 66 768
Size: 66 702
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 642 768
Size: 642 126
TatI w'GTAC_w
Cuts at: 0 231 312 768
Size: 231 81 456
Fragments arranged by size:
456 231 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 107 139 768
Size: 107 32 629
Fragments arranged by size:
629 107 32
XmaI C'CCGG_G
Cuts at: 0 8 768
Size: 8 760
Enzymes that do cut:
AcuI AlfI AloI AlwNI ApoI AvaI AvrII BglI
BmrI BpuEI BsaI BsaAI BsaXI BsgI BsmI BsrDI
BstAPI Bsu36I BtsI DraIII EarI EciI Eco57MI EcoO109I
EcoRI HaeII MfeI MmeI MslI MspA1I PmlI PpiI
PpuMI ScaI SmaI SmlI StyI TaqII TatI TstI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI ApaI ApaLI AscI AseI AsiSI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmtI
BplI BpmI Bpu10I BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI ClaI CspCI DraI DrdI
EaeI EagI EcoICRI EcoNI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI
XhoI XmnI ZraI