Restriction map for
pFN21AE6278
(Linear) (Six-base) MAPSORT of: KIEE6278.seq Check: 8577 from: 1 to: 684
KIEE6278.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 264 684
Size: 150 114 420
Fragments arranged by size:
420 150 114
AflIII A'CryG_T
Cuts at: 0 50 684
Size: 50 634
AleI CACnn'nnGTG
Cuts at: 0 53 684
Size: 53 631
ApaI G_GGCC'C
Cuts at: 0 66 177 684
Size: 66 111 507
Fragments arranged by size:
507 111 66
BanI G'GyrC_C
Cuts at: 0 112 139 684
Size: 112 27 545
Fragments arranged by size:
545 112 27
BanII G_rGCy'C
Cuts at: 0 66 177 682 684
Size: 66 111 505 2
Fragments arranged by size:
505 111 66 2
BbvCI CC'TCA_GC
Cuts at: 0 326 684
Size: 326 358
Bme1580I G_kGCm'C
Cuts at: 0 66 177 684
Size: 66 111 507
Fragments arranged by size:
507 111 66
BmrI ACTGGGnnnn_n'
Cuts at: 0 228 382 684
Size: 228 154 302
Fragments arranged by size:
302 228 154
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 82 684
Size: 82 602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 684
Size: 482 202
Bpu10I CC'TnA_GC
Cuts at: 0 326 684
Size: 326 358
BsaAI yAC'GTr
Cuts at: 0 51 684
Size: 51 633
BsaWI w'CCGG_w
Cuts at: 0 32 684
Size: 32 652
BseYI C'CCAG_C
Cuts at: 0 76 80 505 684
Size: 76 4 425 179
Fragments arranged by size:
425 179 76 4
BsiHKAI G_wGCw'C
Cuts at: 0 50 684
Size: 50 634
Bsp1286I G_dGCh'C
Cuts at: 0 50 66 177 682 684
Size: 50 16 111 505 2
Fragments arranged by size:
505 111 50 16 2
BspEI T'CCGG_A
Cuts at: 0 32 684
Size: 32 652
BsrDI GCAATG_nn'
Cuts at: 0 612 684
Size: 612 72
BsrFI r'CCGG_y
Cuts at: 0 359 684
Size: 359 325
BsrGI T'GTAC_A
Cuts at: 0 557 684
Size: 557 127
BstXI CCAn_nnnn'nTGG
Cuts at: 0 84 684
Size: 84 600
BtsI GCAGTG_nn'
Cuts at: 0 99 684
Size: 99 585
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 264 482 684
Size: 150 114 218 202
Fragments arranged by size:
218 202 150 114
EcoO109I rG'GnC_Cy
Cuts at: 0 62 63 173 174 183 622 684
Size: 62 1 110 1 9 439 62
Fragments arranged by size:
439 110 62 62 9 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 393 396 684
Size: 393 3 288
Fragments arranged by size:
393 288 3
MslI CAynn'nnrTG
Cuts at: 0 53 202 684
Size: 53 149 482
Fragments arranged by size:
482 149 53
MspA1I CmG'CkG
Cuts at: 0 80 684
Size: 80 604
PasI CC'CwG_GG
Cuts at: 0 17 684
Size: 17 667
PflMI CCAn_nnn'nTGG
Cuts at: 0 218 684
Size: 218 466
PmlI CAC'GTG
Cuts at: 0 51 684
Size: 51 633
PpuMI rG'GwC_Cy
Cuts at: 0 183 622 684
Size: 183 439 62
Fragments arranged by size:
439 183 62
PspOMI G'GGCC_C
Cuts at: 0 62 173 684
Size: 62 111 511
Fragments arranged by size:
511 111 62
PstI C_TGCA'G
Cuts at: 0 155 320 668 684
Size: 155 165 348 16
Fragments arranged by size:
348 165 155 16
PvuII CAG'CTG
Cuts at: 0 80 684
Size: 80 604
SanDI GG'GwC_CC
Cuts at: 0 183 684
Size: 183 501
ScaI AGT'ACT
Cuts at: 0 124 217 684
Size: 124 93 467
Fragments arranged by size:
467 124 93
SfcI C'TryA_G
Cuts at: 0 151 316 664 684
Size: 151 165 348 20
Fragments arranged by size:
348 165 151 20
StuI AGG'CCT
Cuts at: 0 529 684
Size: 529 155
StyI C'CwwG_G
Cuts at: 0 108 684
Size: 108 576
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 72 229 684
Size: 72 157 455
Fragments arranged by size:
455 157 72
TatI w'GTAC_w
Cuts at: 0 122 215 557 684
Size: 122 93 342 127
Fragments arranged by size:
342 127 122 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 430 462 684
Size: 430 32 222
Fragments arranged by size:
430 222 32
XmnI GAAnn'nnTTC
Cuts at: 0 376 684
Size: 376 308
Enzymes that do cut:
AcuI AflIII AleI ApaI BanI BanII BbvCI Bme1580I
BmrI BplI BpmI Bpu10I BsaAI BsaWI BseYI BsiHKAI
Bsp1286I BspEI BsrDI BsrFI BsrGI BstXI BtsI Eco57MI
EcoO109I FalI MslI MspA1I PasI PflMI PmlI PpuMI
PspOMI PstI PvuII SanDI ScaI SfcI StuI StyI
TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI
BpuEI BsaI BsaBI BsaHI BsaXI BseRI BsgI BsiEI
BsiWI BsmI BsmBI BspHI BspMI BsrBI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI