(Linear) (Six-base) MAPSORT of: KIEE6277.seq  Check: 2581  from: 1  to: 672



KIEE6277.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:29  ..

AclI AA'CG_TT

Cuts at:      0     537     672 
   Size:        537     135
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     672 
   Size:        101     571
 
ApoI r'AATT_y

Cuts at:      0     525     672 
   Size:        525     147
 
BanI G'GyrC_C

Cuts at:      0      32     672 
   Size:         32     640
 
BglII A'GATC_T

Cuts at:      0     647     672 
   Size:        647      25
 
BmgBI CAC'GTC

Cuts at:      0     133     672 
   Size:        133     539
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275     672 
   Size:        275     397
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     101     672 
   Size:        101     571
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     672 
   Size:        101     571
 
BsiHKAI G_wGCw'C

Cuts at:      0     669     672 
   Size:        669       3
 
Bsp1286I G_dGCh'C

Cuts at:      0     669     672 
   Size:        669       3
 
BsrFI r'CCGG_y

Cuts at:      0      29     672 
   Size:         29     643
 
BstYI r'GATC_y

Cuts at:      0     647     672 
   Size:        647      25
 
BtgI C'CryG_G

Cuts at:      0     202     672 
   Size:        202     470
 
BtsI GCAGTG_nn'

Cuts at:      0     400     672 
   Size:        400     272
 
EaeI y'GGCC_r

Cuts at:      0       2     672 
   Size:          2     670
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     275     672 
   Size:        101     174     397

  Fragments arranged by size: 

                 397     174     101
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     297     672 
   Size:        297     375
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     639     671     672 
   Size:        639      32       1

  Fragments arranged by size: 

                 639      32       1
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     558     672 
   Size:        558     114
 
MscI TGG'CCA

Cuts at:      0       4     672 
   Size:          4     668
 
MslI CAynn'nnrTG

Cuts at:      0      68     672 
   Size:         68     604
 
NcoI C'CATG_G

Cuts at:      0     202     672 
   Size:        202     470
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     203     672 
   Size:        203     469
 
SspI AAT'ATT

Cuts at:      0     438     672 
   Size:        438     234
 
StyI C'CwwG_G

Cuts at:      0     202     672 
   Size:        202     470
 

 Enzymes that do cut:

     AclI     AcuI     ApoI     BanI    BglII    BmgBI     BpmI    BseRI
     BsgI  BsiHKAI Bsp1286I    BsrFI    BstYI     BtgI     BtsI     EaeI
  Eco57MI     FalI    Hin4I     MmeI     MscI     MslI     NcoI    PflMI
     SspI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI