(Linear) (Six-base) MAPSORT of: KIEE6272.seq  Check: 1197  from: 1  to: 546



KIEE6272.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:29  ..

AccI GT'mk_AC

Cuts at:      0     398     546 
   Size:        398     148
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     425     546 
   Size:        218     207     121

  Fragments arranged by size: 

                 218     207     121
 
AlwNI CAG_nnn'CTG

Cuts at:      0     456     546 
   Size:        456      90
 
AvaI C'yCGr_G

Cuts at:      0     242     546 
   Size:        242     304
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      60     546 
   Size:         60     486
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     472     546 
   Size:        472      74
 
BsrDI GCAATG_nn'

Cuts at:      0     404     546 
   Size:        404     142
 
DraI TTT'AAA

Cuts at:      0     168     546 
   Size:        168     378
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     425     546 
   Size:        218     207     121

  Fragments arranged by size: 

                 218     207     121
 
EcoO109I rG'GnC_Cy

Cuts at:      0      15     237     546 
   Size:         15     222     309

  Fragments arranged by size: 

                 309     222      15
 
PpuMI rG'GwC_Cy

Cuts at:      0     237     546 
   Size:        237     309
 
PspXI vC'TCGA_Gb

Cuts at:      0     242     546 
   Size:        242     304
 
SmlI C'TyrA_G

Cuts at:      0     242     487     546 
   Size:        242     245      59

  Fragments arranged by size: 

                 245     242      59
 
StyI C'CwwG_G

Cuts at:      0     233     546 
   Size:        233     313
 
TatI w'GTAC_w

Cuts at:      0     509     546 
   Size:        509      37
 
XhoI C'TCGA_G

Cuts at:      0     242     546 
   Size:        242     304
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     AvaI     BmrI    BpuEI    BsrDI     DraI
  Eco57MI EcoO109I    PpuMI    PspXI     SmlI     StyI     TatI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI     ZraI