Restriction map for
pFN21AE6262
(Linear) (Six-base) MAPSORT of: KIEE6262.seq Check: 4574 from: 1 to: 834
KIEE6262.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 818 834
Size: 685 133 16
Fragments arranged by size:
685 133 16
ApaI G_GGCC'C
Cuts at: 0 105 834
Size: 105 729
ApoI r'AATT_y
Cuts at: 0 745 780 834
Size: 745 35 54
Fragments arranged by size:
745 54 35
AvaI C'yCGr_G
Cuts at: 0 104 173 834
Size: 104 69 661
Fragments arranged by size:
661 104 69
BamHI G'GATC_C
Cuts at: 0 321 834
Size: 321 513
BanI G'GyrC_C
Cuts at: 0 95 834
Size: 95 739
BanII G_rGCy'C
Cuts at: 0 105 834
Size: 105 729
BbeI G_GCGC'C
Cuts at: 0 99 834
Size: 99 735
BclI T'GATC_A
Cuts at: 0 626 834
Size: 626 208
BfrBI ATG'CAT
Cuts at: 0 672 834
Size: 672 162
BglII A'GATC_T
Cuts at: 0 255 834
Size: 255 579
Bme1580I G_kGCm'C
Cuts at: 0 105 834
Size: 105 729
BmrI ACTGGGnnnn_n'
Cuts at: 0 372 834
Size: 372 462
BsaBI GATnn'nnATC
Cuts at: 0 182 834
Size: 182 652
BsaHI Gr'CG_yC
Cuts at: 0 96 116 834
Size: 96 20 718
Fragments arranged by size:
718 96 20
BsaWI w'CCGG_w
Cuts at: 0 51 834
Size: 51 783
BseYI C'CCAG_C
Cuts at: 0 17 834
Size: 17 817
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 834
Size: 272 562
BsmI GAATG_Cn'
Cuts at: 0 827 834
Size: 827 7
Bsp1286I G_dGCh'C
Cuts at: 0 105 834
Size: 105 729
BsrBI CCG'CTC
Cuts at: 0 123 138 834
Size: 123 15 696
Fragments arranged by size:
696 123 15
BsrGI T'GTAC_A
Cuts at: 0 429 834
Size: 429 405
BssHII G'CGCG_C
Cuts at: 0 168 834
Size: 168 666
BstYI r'GATC_y
Cuts at: 0 255 313 321 834
Size: 255 58 8 513
Fragments arranged by size:
513 255 58 8
BtgI C'CryG_G
Cuts at: 0 300 752 834
Size: 300 452 82
Fragments arranged by size:
452 300 82
DrdI GACnn_nn'nnGTC
Cuts at: 0 373 834
Size: 373 461
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 818 834
Size: 685 133 16
Fragments arranged by size:
685 133 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 286 834
Size: 286 548
EcoO109I rG'GnC_Cy
Cuts at: 0 366 834
Size: 366 468
EcoRI G'AATT_C
Cuts at: 0 745 834
Size: 745 89
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 486 834
Size: 486 348
HaeII r_GCGC'y
Cuts at: 0 91 99 834
Size: 91 8 735
Fragments arranged by size:
735 91 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 834
Size: 30 804
HindIII A'AGCT_T
Cuts at: 0 693 834
Size: 693 141
KasI G'GCGC_C
Cuts at: 0 95 834
Size: 95 739
MslI CAynn'nnrTG
Cuts at: 0 671 834
Size: 671 163
MspA1I CmG'CkG
Cuts at: 0 132 450 834
Size: 132 318 384
Fragments arranged by size:
384 318 132
NarI GG'CG_CC
Cuts at: 0 96 834
Size: 96 738
NcoI C'CATG_G
Cuts at: 0 300 752 834
Size: 300 452 82
Fragments arranged by size:
452 300 82
NsiI A_TGCA'T
Cuts at: 0 674 834
Size: 674 160
NspI r_CATG'y
Cuts at: 0 672 834
Size: 672 162
PpuMI rG'GwC_Cy
Cuts at: 0 366 834
Size: 366 468
PshAI GACnn'nnGTC
Cuts at: 0 764 834
Size: 764 70
PsiI TTA'TAA
Cuts at: 0 221 787 834
Size: 221 566 47
Fragments arranged by size:
566 221 47
PspOMI G'GGCC_C
Cuts at: 0 101 834
Size: 101 733
PstI C_TGCA'G
Cuts at: 0 74 834
Size: 74 760
SanDI GG'GwC_CC
Cuts at: 0 366 834
Size: 366 468
SfcI C'TryA_G
Cuts at: 0 70 834
Size: 70 764
SfoI GGC'GCC
Cuts at: 0 97 834
Size: 97 737
SmaI CCC'GGG
Cuts at: 0 106 175 834
Size: 106 69 659
Fragments arranged by size:
659 106 69
StyI C'CwwG_G
Cuts at: 0 300 752 834
Size: 300 452 82
Fragments arranged by size:
452 300 82
TatI w'GTAC_w
Cuts at: 0 429 543 834
Size: 429 114 291
Fragments arranged by size:
429 291 114
XmaI C'CCGG_G
Cuts at: 0 104 173 834
Size: 104 69 661
Fragments arranged by size:
661 104 69
XmnI GAAnn'nnTTC
Cuts at: 0 662 834
Size: 662 172
Enzymes that do cut:
AcuI ApaI ApoI AvaI BamHI BanI BanII BbeI
BclI BfrBI BglII Bme1580I BmrI BsaBI BsaHI BsaWI
BseYI BsgI BsmI Bsp1286I BsrBI BsrGI BssHII BstYI
BtgI DrdI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HindIII KasI MslI MspA1I NarI NcoI NsiI
NspI PpuMI PshAI PsiI PspOMI PstI SanDI SfcI
SfoI SmaI StyI TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI AvrII BaeI BbsI BbvCI BcgI BciVI
BglI BlpI BmgBI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspHI BspMI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII EaeI EagI EarI EciI EcoICRI EcoRV
FseI FspI FspAI HincII HpaI KpnI MfeI MluI
MmeI MscI NaeI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI ZraI