(Linear) (Six-base) MAPSORT of: KIEE6261.seq  Check: 9297  from: 1  to: 741



KIEE6261.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     217     295     741 
   Size:        217      78     446

  Fragments arranged by size: 

                 446     217      78
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     219     230     731     741 
   Size:        118     101      11     501      10

  Fragments arranged by size: 

                 501     118     101      11      10
 
AflIII A'CryG_T

Cuts at:      0     519     741 
   Size:        519     222
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     698     741 
   Size:        698      43
 
BsiHKAI G_wGCw'C

Cuts at:      0      78     741 
   Size:         78     663
 
Bsp1286I G_dGCh'C

Cuts at:      0      78     741 
   Size:         78     663
 
BspHI T'CATG_A

Cuts at:      0      53     741 
   Size:         53     688
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     217     295     741 
   Size:        217      78     446

  Fragments arranged by size: 

                 446     217      78
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     423     741 
   Size:        423     318
 
BstYI r'GATC_y

Cuts at:      0     137     726     741 
   Size:        137     589      15

  Fragments arranged by size: 

                 589     137      15
 
BtsI GCAGTG_nn'

Cuts at:      0     428     741 
   Size:        428     313
 
DraI TTT'AAA

Cuts at:      0      68     741 
   Size:         68     673
 
EarI CTCTTCn'nnn_

Cuts at:      0     128     584     707     741 
   Size:        128     456     123      34

  Fragments arranged by size: 

                 456     128     123      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     219     230     698     731     741 
   Size:        118     101      11     468      33      10

  Fragments arranged by size: 

                 468     118     101      33      11      10
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     668     741 
   Size:        668      73
 
MslI CAynn'nnrTG

Cuts at:      0     335     741 
   Size:        335     406
 
MspA1I CmG'CkG

Cuts at:      0     676     741 
   Size:        676      65
 
NruI TCG'CGA

Cuts at:      0      36     741 
   Size:         36     705
 
NspI r_CATG'y

Cuts at:      0     523     741 
   Size:        523     218
 
PciI A'CATG_T

Cuts at:      0     519     741 
   Size:        519     222
 
PstI C_TGCA'G

Cuts at:      0     455     741 
   Size:        455     286
 
PvuII CAG'CTG

Cuts at:      0     676     741 
   Size:        676      65
 
SfcI C'TryA_G

Cuts at:      0     242     451     597     741 
   Size:        242     209     146     144

  Fragments arranged by size: 

                 242     209     146     144
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      29     741 
   Size:         29     712
 
XmnI GAAnn'nnTTC

Cuts at:      0      26     741 
   Size:         26     715
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     BpmI  BsiHKAI Bsp1286I    BspHI    BspMI
    BstXI    BstYI     BtsI     DraI     EarI  Eco57MI     MmeI     MslI
   MspA1I     NruI     NspI     PciI     PstI    PvuII     SfcI    TaqII
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI