(Linear) (Six-base) MAPSORT of: KIEE6258.seq  Check: 3263  from: 1  to: 750



KIEE6258.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

AccI GT'mk_AC

Cuts at:      0     695     750 
   Size:        695      55
 
AleI CACnn'nnGTG

Cuts at:      0     556     750 
   Size:        556     194
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     201     750 
   Size:        201     549
 
AlwNI CAG_nnn'CTG

Cuts at:      0     268     750 
   Size:        268     482
 
ApaI G_GGCC'C

Cuts at:      0     616     750 
   Size:        616     134
 
BamHI G'GATC_C

Cuts at:      0     663     725     750 
   Size:        663      62      25

  Fragments arranged by size: 

                 663      62      25
 
BanI G'GyrC_C

Cuts at:      0     415     750 
   Size:        415     335
 
BanII G_rGCy'C

Cuts at:      0     616     750 
   Size:        616     134
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     130     750 
   Size:        130     620
 
Bme1580I G_kGCm'C

Cuts at:      0     616     750 
   Size:        616     134
 
BmgBI CAC'GTC

Cuts at:      0     449     750 
   Size:        449     301
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     453     750 
   Size:        453     297
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     750 
   Size:        214     536
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     750 
   Size:        175     575
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      49     750 
   Size:         49     701
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     253     384     750 
   Size:        253     131     366

  Fragments arranged by size: 

                 366     253     131
 
BseYI C'CCAG_C

Cuts at:      0      95     437     750 
   Size:         95     342     313

  Fragments arranged by size: 

                 342     313      95
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     592     750 
   Size:        592     158
 
BsiHKAI G_wGCw'C

Cuts at:      0     512     750 
   Size:        512     238
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     727     750 
   Size:        727      23
 
Bsp1286I G_dGCh'C

Cuts at:      0     512     616     750 
   Size:        512     104     134

  Fragments arranged by size: 

                 512     134     104
 
BsrDI GCAATG_nn'

Cuts at:      0     330     750 
   Size:        330     420
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     422     750 
   Size:        422     328
 
BstYI r'GATC_y

Cuts at:      0     663     725     750 
   Size:        663      62      25

  Fragments arranged by size: 

                 663      62      25
 
BtgI C'CryG_G

Cuts at:      0     554     750 
   Size:        554     196
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     138     335     498     750 
   Size:        138     197     163     252

  Fragments arranged by size: 

                 252     197     163     138
 
BtsI GCAGTG_nn'

Cuts at:      0     183     407     750 
   Size:        183     224     343

  Fragments arranged by size: 

                 343     224     183
 
DraIII CAC_nnn'GTG

Cuts at:      0     403     750 
   Size:        403     347
 
EarI CTCTTCn'nnn_

Cuts at:      0     641     750 
   Size:        641     109
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     750 
   Size:        214     536
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     232     750 
   Size:        232     518
 
EcoO109I rG'GnC_Cy

Cuts at:      0     612     613     750 
   Size:        612       1     137

  Fragments arranged by size: 

                 612     137       1
 
MslI CAynn'nnrTG

Cuts at:      0     323     337     556     750 
   Size:        323      14     219     194

  Fragments arranged by size: 

                 323     219     194      14
 
MspA1I CmG'CkG

Cuts at:      0     422     750 
   Size:        422     328
 
NcoI C'CATG_G

Cuts at:      0     554     750 
   Size:        554     196
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     368     750 
   Size:        368     382
 
PfoI T'CCnGG_A

Cuts at:      0     728     750 
   Size:        728      22
 
PspOMI G'GGCC_C

Cuts at:      0     612     750 
   Size:        612     138
 
PstI C_TGCA'G

Cuts at:      0      14     750 
   Size:         14     736
 
PvuII CAG'CTG

Cuts at:      0     422     750 
   Size:        422     328
 
SfcI C'TryA_G

Cuts at:      0      10     750 
   Size:         10     740
 
SmlI C'TyrA_G

Cuts at:      0     190     750 
   Size:        190     560
 
StyI C'CwwG_G

Cuts at:      0     554     750 
   Size:        554     196
 

 Enzymes that do cut:

     AccI     AleI     AlfI    AlwNI     ApaI    BamHI     BanI    BanII
     BbsI Bme1580I    BmgBI     BmrI     BpmI    BpuEI     BsaI    BseRI
    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BsrDI   BstAPI    BstYI
     BtgI    BtgZI     BtsI   DraIII     EarI  Eco57MI    EcoNI EcoO109I
     MslI   MspA1I     NcoI    PflMI     PfoI   PspOMI     PstI    PvuII
     SfcI     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI     BmtI     BplI   Bpu10I    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI