Restriction map for
pFN21AE6256
(Linear) (Six-base) MAPSORT of: KIEE6256.seq Check: 3804 from: 1 to: 627
KIEE6256.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 95 627
Size: 95 532
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 627
Size: 46 581
AfeI AGC'GCT
Cuts at: 0 491 627
Size: 491 136
ApaI G_GGCC'C
Cuts at: 0 65 627
Size: 65 562
AvaI C'yCGr_G
Cuts at: 0 70 627
Size: 70 557
BanII G_rGCy'C
Cuts at: 0 65 627
Size: 65 562
BbsI GAAGACnn'nnnn_
Cuts at: 0 309 627
Size: 309 318
BglI GCCn_nnn'nGGC
Cuts at: 0 274 388 627
Size: 274 114 239
Fragments arranged by size:
274 239 114
BglII A'GATC_T
Cuts at: 0 437 627
Size: 437 190
BlpI GC'TnA_GC
Cuts at: 0 608 627
Size: 608 19
Bme1580I G_kGCm'C
Cuts at: 0 65 627
Size: 65 562
BmrI ACTGGGnnnn_n'
Cuts at: 0 112 627
Size: 112 515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 307 550 627
Size: 241 66 243 77
Fragments arranged by size:
243 241 77 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 627
Size: 391 236
BsaI GGTCTCn'nnnn_
Cuts at: 0 49 627
Size: 49 578
BsaWI w'CCGG_w
Cuts at: 0 200 627
Size: 200 427
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 508 538 627
Size: 508 30 89
Fragments arranged by size:
508 89 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 532 535 627
Size: 532 3 92
Fragments arranged by size:
532 92 3
BseYI C'CCAG_C
Cuts at: 0 189 627
Size: 189 438
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 627
Size: 451 176
Bsp1286I G_dGCh'C
Cuts at: 0 65 627
Size: 65 562
BspEI T'CCGG_A
Cuts at: 0 200 627
Size: 200 427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 95 627
Size: 95 532
BsrBI CCG'CTC
Cuts at: 0 345 627
Size: 345 282
BsrGI T'GTAC_A
Cuts at: 0 590 627
Size: 590 37
BstYI r'GATC_y
Cuts at: 0 437 536 627
Size: 437 99 91
Fragments arranged by size:
437 99 91
BtgI C'CryG_G
Cuts at: 0 38 417 627
Size: 38 379 210
Fragments arranged by size:
379 210 38
ClaI AT'CG_AT
Cuts at: 0 572 627
Size: 572 55
EaeI y'GGCC_r
Cuts at: 0 11 627
Size: 11 616
EarI CTCTTCn'nnn_
Cuts at: 0 550 627
Size: 550 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 529 627
Size: 529 98
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 241 307 550 627
Size: 46 195 66 243 77
Fragments arranged by size:
243 195 77 66 46
EcoO109I rG'GnC_Cy
Cuts at: 0 62 113 627
Size: 62 51 514
Fragments arranged by size:
514 62 51
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 495 627
Size: 495 132
HaeII r_GCGC'y
Cuts at: 0 7 280 493 627
Size: 7 273 213 134
Fragments arranged by size:
273 213 134 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 627
Size: 64 32 531
Fragments arranged by size:
531 64 32
HincII GTy'rAC
Cuts at: 0 378 555 627
Size: 378 177 72
Fragments arranged by size:
378 177 72
MslI CAynn'nnrTG
Cuts at: 0 598 627
Size: 598 29
MspA1I CmG'CkG
Cuts at: 0 33 40 627
Size: 33 7 587
Fragments arranged by size:
587 33 7
NcoI C'CATG_G
Cuts at: 0 417 627
Size: 417 210
PfoI T'CCnGG_A
Cuts at: 0 218 627
Size: 218 409
PpuMI rG'GwC_Cy
Cuts at: 0 113 627
Size: 113 514
PspOMI G'GGCC_C
Cuts at: 0 61 627
Size: 61 566
PspXI vC'TCGA_Gb
Cuts at: 0 70 627
Size: 70 557
PstI C_TGCA'G
Cuts at: 0 212 627
Size: 212 415
SacII CC_GC'GG
Cuts at: 0 41 627
Size: 41 586
SanDI GG'GwC_CC
Cuts at: 0 113 627
Size: 113 514
SfcI C'TryA_G
Cuts at: 0 208 627
Size: 208 419
SmlI C'TyrA_G
Cuts at: 0 70 406 627
Size: 70 336 221
Fragments arranged by size:
336 221 70
StuI AGG'CCT
Cuts at: 0 299 391 627
Size: 299 92 236
Fragments arranged by size:
299 236 92
StyI C'CwwG_G
Cuts at: 0 108 386 417 497 627
Size: 108 278 31 80 130
Fragments arranged by size:
278 130 108 80 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 610 627
Size: 610 17
TatI w'GTAC_w
Cuts at: 0 590 627
Size: 590 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 508 540 627
Size: 508 32 87
Fragments arranged by size:
508 87 32
XhoI C'TCGA_G
Cuts at: 0 70 627
Size: 70 557
XmnI GAAnn'nnTTC
Cuts at: 0 439 627
Size: 439 188
Enzymes that do cut:
AarI AcuI AfeI ApaI AvaI BanII BbsI BglI
BglII BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaWI
BsaXI BseRI BseYI BsgI Bsp1286I BspEI BspMI BsrBI
BsrGI BstYI BtgI ClaI EaeI EarI EciI Eco57MI
EcoO109I FalI HaeII Hin4I HincII MslI MspA1I NcoI
PfoI PpuMI PspOMI PspXI PstI SacII SanDI SfcI
SmlI StuI StyI TaqII TatI TstI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BmgBI BmtI BplI Bpu10I BsaAI
BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI CspCI DraI DraIII DrdI
EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PsrI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XcmI XmaI ZraI