(Linear) (Six-base) MAPSORT of: KIEE6255.seq  Check: 9100  from: 1  to: 678



KIEE6255.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

AccI GT'mk_AC

Cuts at:      0     332     548     678 
   Size:        332     216     130

  Fragments arranged by size: 

                 332     216     130
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     678 
   Size:        172     506
 
AflIII A'CryG_T

Cuts at:      0     483     678 
   Size:        483     195
 
AlwNI CAG_nnn'CTG

Cuts at:      0     342     369     678 
   Size:        342      27     309

  Fragments arranged by size: 

                 342     309      27
 
AvaI C'yCGr_G

Cuts at:      0     457     678 
   Size:        457     221
 
BanII G_rGCy'C

Cuts at:      0     258     678 
   Size:        258     420
 
BbvCI CC'TCA_GC

Cuts at:      0     101     678 
   Size:        101     577
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     346     554     678 
   Size:        346     208     124

  Fragments arranged by size: 

                 346     208     124
 
Bpu10I CC'TnA_GC

Cuts at:      0     101     678 
   Size:        101     577
 
BsaHI Gr'CG_yC

Cuts at:      0     129     678 
   Size:        129     549
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     344     374     404     434     678 
   Size:        344      30      30      30     244

  Fragments arranged by size: 

                 344     244      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     412     678 
   Size:        412     266
 
BsiHKAI G_wGCw'C

Cuts at:      0     362     678 
   Size:        362     316
 
BsmI GAATG_Cn'

Cuts at:      0     531     678 
   Size:        531     147
 
Bsp1286I G_dGCh'C

Cuts at:      0     258     362     678 
   Size:        258     104     316

  Fragments arranged by size: 

                 316     258     104
 
BsrFI r'CCGG_y

Cuts at:      0     407     678 
   Size:        407     271
 
BsrGI T'GTAC_A

Cuts at:      0     441     678 
   Size:        441     237
 
BssSI C'ACGA_G

Cuts at:      0     133     678 
   Size:        133     545
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     621     678 
   Size:        621      57
 
BstYI r'GATC_y

Cuts at:      0     600     678 
   Size:        600      78
 
ClaI AT'CG_AT

Cuts at:      0      89     678 
   Size:         89     589
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     328     678 
   Size:        328     350
 
EaeI y'GGCC_r

Cuts at:      0     409     678 
   Size:        409     269
 
EarI CTCTTCn'nnn_

Cuts at:      0     101     678 
   Size:        101     577
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     346     554     678 
   Size:        172     174     208     124

  Fragments arranged by size: 

                 208     174     172     124
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     430     678 
   Size:        430     248
 
EcoO109I rG'GnC_Cy

Cuts at:      0     321     678 
   Size:        321     357
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     256     288     587     619     678 
   Size:        256      32     299      32      59

  Fragments arranged by size: 

                 299     256      59      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     399     678 
   Size:        399     279
 
MspA1I CmG'CkG

Cuts at:      0     532     678 
   Size:        532     146
 
NaeI GCC'GGC

Cuts at:      0     409     678 
   Size:        409     269
 
NgoMIV G'CCGG_C

Cuts at:      0     407     678 
   Size:        407     271
 
NspI r_CATG'y

Cuts at:      0     487     678 
   Size:        487     191
 
PciI A'CATG_T

Cuts at:      0     483     678 
   Size:        483     195
 
PpuMI rG'GwC_Cy

Cuts at:      0     321     678 
   Size:        321     357
 
PvuII CAG'CTG

Cuts at:      0     532     678 
   Size:        532     146
 
SfcI C'TryA_G

Cuts at:      0     549     678 
   Size:        549     129
 
StyI C'CwwG_G

Cuts at:      0      95     584     678 
   Size:         95     489      94

  Fragments arranged by size: 

                 489      95      94
 
TatI w'GTAC_w

Cuts at:      0     441     461     678 
   Size:        441      20     217

  Fragments arranged by size: 

                 441     217      20
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     119     151     404     436     678 
   Size:        119      32     253      32     242

  Fragments arranged by size: 

                 253     242     119      32      32
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     AvaI    BanII    BbvCI     BpmI
   Bpu10I    BsaHI    BsaXI    BseRI  BsiHKAI     BsmI Bsp1286I    BsrFI
    BsrGI    BssSI   BstAPI    BstYI     ClaI     DrdI     EaeI     EarI
  Eco57MI    EcoNI EcoO109I    Hin4I     MmeI   MspA1I     NaeI   NgoMIV
     NspI     PciI    PpuMI    PvuII     SfcI     StyI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     EagI     EciI
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI