(Linear) (Six-base) MAPSORT of: KIEE6253.seq  Check: 8974  from: 1  to: 642



KIEE6253.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

AccI GT'mk_AC

Cuts at:      0      98     642 
   Size:         98     544
 
Acc65I G'GTAC_C

Cuts at:      0      52     642 
   Size:         52     590
 
AlwNI CAG_nnn'CTG

Cuts at:      0      43     168     642 
   Size:         43     125     474

  Fragments arranged by size: 

                 474     125      43
 
ApoI r'AATT_y

Cuts at:      0     617     642 
   Size:        617      25
 
BanI G'GyrC_C

Cuts at:      0      52     642 
   Size:         52     590
 
BanII G_rGCy'C

Cuts at:      0     502     642 
   Size:        502     140
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     344     642 
   Size:        344     298
 
BsaWI w'CCGG_w

Cuts at:      0     477     642 
   Size:        477     165
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     484     514     642 
   Size:        484      30     128

  Fragments arranged by size: 

                 484     128      30
 
BseYI C'CCAG_C

Cuts at:      0      30     642 
   Size:         30     612
 
BsmI GAATG_Cn'

Cuts at:      0     177     642 
   Size:        177     465
 
Bsp1286I G_dGCh'C

Cuts at:      0     502     642 
   Size:        502     140
 
BsrDI GCAATG_nn'

Cuts at:      0     185     374     594     642 
   Size:        185     189     220      48

  Fragments arranged by size: 

                 220     189     185      48
 
BstYI r'GATC_y

Cuts at:      0     164     642 
   Size:        164     478
 
BtgI C'CryG_G

Cuts at:      0      48     642 
   Size:         48     594
 
BtsI GCAGTG_nn'

Cuts at:      0      69     642 
   Size:         69     573
 
EarI CTCTTCn'nnn_

Cuts at:      0      74     468     642 
   Size:         74     394     174

  Fragments arranged by size: 

                 394     174      74
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     261     642 
   Size:        261     381
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      19      51     642 
   Size:         19      32     591

  Fragments arranged by size: 

                 591      32      19
 
KpnI G_GTAC'C

Cuts at:      0      56     642 
   Size:         56     586
 
MfeI C'AATT_G

Cuts at:      0     389     642 
   Size:        389     253
 
MslI CAynn'nnrTG

Cuts at:      0     577     642 
   Size:        577      65
 
NcoI C'CATG_G

Cuts at:      0      48     642 
   Size:         48     594
 
SapI GCTCTTCn'nnn_

Cuts at:      0      74     642 
   Size:         74     568
 
SfcI C'TryA_G

Cuts at:      0     297     642 
   Size:        297     345
 
SpeI A'CTAG_T

Cuts at:      0     396     642 
   Size:        396     246
 
StyI C'CwwG_G

Cuts at:      0      48     642 
   Size:         48     594
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     184     642 
   Size:        184     458
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     109     642 
   Size:        109     533
 

 Enzymes that do cut:

     AccI   Acc65I    AlwNI     ApoI     BanI    BanII    BciVI    BsaWI
    BsaXI    BseYI     BsmI Bsp1286I    BsrDI    BstYI     BtgI     BtsI
     EarI     FalI    Hin4I     KpnI     MfeI     MslI     NcoI     SapI
     SfcI     SpeI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SphI     SrfI     SspI     StuI     SwaI     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI