Restriction map for
pFN21AE6250
(Linear) (Six-base) MAPSORT of: KIEE6250.seq Check: 3776 from: 1 to: 774
KIEE6250.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AatII G_ACGT'C
Cuts at: 0 416 774
Size: 416 358
AflIII A'CryG_T
Cuts at: 0 653 774
Size: 653 121
ApaI G_GGCC'C
Cuts at: 0 12 774
Size: 12 762
AvaI C'yCGr_G
Cuts at: 0 161 526 774
Size: 161 365 248
Fragments arranged by size:
365 248 161
BanII G_rGCy'C
Cuts at: 0 12 45 160 774
Size: 12 33 115 614
Fragments arranged by size:
614 115 33 12
Bme1580I G_kGCm'C
Cuts at: 0 12 774
Size: 12 762
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 219 774
Size: 219 555
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 774
Size: 410 364
BsaI GGTCTCn'nnnn_
Cuts at: 0 207 774
Size: 207 567
BsaAI yAC'GTr
Cuts at: 0 561 774
Size: 561 213
BsaHI Gr'CG_yC
Cuts at: 0 413 676 774
Size: 413 263 98
Fragments arranged by size:
413 263 98
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 597 627 774
Size: 597 30 147
Fragments arranged by size:
597 147 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 618 774
Size: 618 156
BseYI C'CCAG_C
Cuts at: 0 24 58 747 774
Size: 24 34 689 27
Fragments arranged by size:
689 34 27 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 774
Size: 157 617
Bsp1286I G_dGCh'C
Cuts at: 0 12 45 160 774
Size: 12 33 115 614
Fragments arranged by size:
614 115 33 12
BsrDI GCAATG_nn'
Cuts at: 0 698 774
Size: 698 76
BstEII G'GTnAC_C
Cuts at: 0 165 774
Size: 165 609
BstXI CCAn_nnnn'nTGG
Cuts at: 0 747 774
Size: 747 27
BtgI C'CryG_G
Cuts at: 0 144 774
Size: 144 630
BtsI GCAGTG_nn'
Cuts at: 0 174 774
Size: 174 600
DrdI GACnn_nn'nnGTC
Cuts at: 0 139 774
Size: 139 635
EarI CTCTTCn'nnn_
Cuts at: 0 88 93 774
Size: 88 5 681
Fragments arranged by size:
681 88 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 774
Size: 410 364
EcoO109I rG'GnC_Cy
Cuts at: 0 9 308 339 774
Size: 9 299 31 435
Fragments arranged by size:
435 299 31 9
HaeII r_GCGC'y
Cuts at: 0 231 774
Size: 231 543
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 199 231 497 529 774
Size: 199 32 266 32 245
Fragments arranged by size:
266 245 199 32 32
MluI A'CGCG_T
Cuts at: 0 653 774
Size: 653 121
NcoI C'CATG_G
Cuts at: 0 144 774
Size: 144 630
PflMI CCAn_nnn'nTGG
Cuts at: 0 197 774
Size: 197 577
PpuMI rG'GwC_Cy
Cuts at: 0 308 339 774
Size: 308 31 435
Fragments arranged by size:
435 308 31
PspOMI G'GGCC_C
Cuts at: 0 8 774
Size: 8 766
PstI C_TGCA'G
Cuts at: 0 602 774
Size: 602 172
SanDI GG'GwC_CC
Cuts at: 0 339 774
Size: 339 435
SapI GCTCTTCn'nnn_
Cuts at: 0 88 774
Size: 88 686
SfcI C'TryA_G
Cuts at: 0 384 598 774
Size: 384 214 176
Fragments arranged by size:
384 214 176
SmaI CCC'GGG
Cuts at: 0 163 774
Size: 163 611
StyI C'CwwG_G
Cuts at: 0 144 278 740 774
Size: 144 134 462 34
Fragments arranged by size:
462 144 134 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 597 629 774
Size: 597 32 145
Fragments arranged by size:
597 145 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 66 774
Size: 66 708
XmaI C'CCGG_G
Cuts at: 0 161 774
Size: 161 613
ZraI GAC'GTC
Cuts at: 0 414 774
Size: 414 360
Enzymes that do cut:
AatII AflIII ApaI AvaI BanII Bme1580I BplI BpmI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI Bsp1286I
BsrDI BstEII BstXI BtgI BtsI DrdI EarI Eco57MI
EcoO109I HaeII Hin4I MluI NcoI PflMI PpuMI PspOMI
PstI SanDI SapI SfcI SmaI StyI TstI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI Bpu10I BpuEI BsaBI BsaWI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII EaeI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI
XhoI XmnI