(Linear) (Six-base) MAPSORT of: KIEE6249.seq  Check: 2846  from: 1  to: 783



KIEE6249.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     419     775     783 
   Size:        419     356       8

  Fragments arranged by size: 

                 419     356       8
 
ApoI r'AATT_y

Cuts at:      0     207     372     534     783 
   Size:        207     165     162     249

  Fragments arranged by size: 

                 249     207     165     162
 
BanII G_rGCy'C

Cuts at:      0     525     783 
   Size:        525     258
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      71     783 
   Size:         71     712
 
BglI GCCn_nnn'nGGC

Cuts at:      0     412     783 
   Size:        412     371
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      24     300     783 
   Size:         24     276     483

  Fragments arranged by size: 

                 483     276      24
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     372     783 
   Size:        372     411
 
BsaAI yAC'GTr

Cuts at:      0     360     783 
   Size:        360     423
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     588     618     783 
   Size:        588      30     165

  Fragments arranged by size: 

                 588     165      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     596     783 
   Size:        596     187
 
BseYI C'CCAG_C

Cuts at:      0     159     783 
   Size:        159     624
 
BsmI GAATG_Cn'

Cuts at:      0     192     783 
   Size:        192     591
 
Bsp1286I G_dGCh'C

Cuts at:      0     525     783 
   Size:        525     258
 
BsrDI GCAATG_nn'

Cuts at:      0      65     783 
   Size:         65     718
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     372     419     775     783 
   Size:        372      47     356       8

  Fragments arranged by size: 

                 372     356      47       8
 
EcoRI G'AATT_C

Cuts at:      0     372     783 
   Size:        372     411
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     214     246     588     620     783 
   Size:        214      32     342      32     163

  Fragments arranged by size: 

                 342     214     163      32      32
 
MspA1I CmG'CkG

Cuts at:      0     669     783 
   Size:        669     114
 
PstI C_TGCA'G

Cuts at:      0     476     783 
   Size:        476     307
 
PvuII CAG'CTG

Cuts at:      0     669     783 
   Size:        669     114
 
ScaI AGT'ACT

Cuts at:      0     385     482     783 
   Size:        385      97     301

  Fragments arranged by size: 

                 385     301      97
 
SfcI C'TryA_G

Cuts at:      0     264     472     783 
   Size:        264     208     311

  Fragments arranged by size: 

                 311     264     208
 
SspI AAT'ATT

Cuts at:      0     343     783 
   Size:        343     440
 
StyI C'CwwG_G

Cuts at:      0     509     783 
   Size:        509     274
 
TatI w'GTAC_w

Cuts at:      0     298     383     480     783 
   Size:        298      85      97     303

  Fragments arranged by size: 

                 303     298      97      85
 

 Enzymes that do cut:

     AcuI     ApoI    BanII    BciVI     BglI     BmrI     BpmI    BsaAI
    BsaXI    BseRI    BseYI     BsmI Bsp1286I    BsrDI  Eco57MI    EcoRI
    Hin4I   MspA1I     PstI    PvuII     ScaI     SfcI     SspI     StyI
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI