(Linear) (Six-base) MAPSORT of: KIEE6248.seq  Check: 6130  from: 1  to: 639



KIEE6248.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

ApoI r'AATT_y

Cuts at:      0      28     252     348     639 
   Size:         28     224      96     291

  Fragments arranged by size: 

                 291     224      96      28
 
AseI AT'TA_AT

Cuts at:      0      73     639 
   Size:         73     566
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     419     639 
   Size:        419     220
 
BfrBI ATG'CAT

Cuts at:      0     472     639 
   Size:        472     167
 
BglII A'GATC_T

Cuts at:      0      82     405     639 
   Size:         82     323     234

  Fragments arranged by size: 

                 323     234      82
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       6     639 
   Size:          6     633
 
Bpu10I CC'TnA_GC

Cuts at:      0     155     639 
   Size:        155     484
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     104     639 
   Size:        104     535
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     360     390     639 
   Size:        360      30     249

  Fragments arranged by size: 

                 360     249      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     373     639 
   Size:        373     266
 
Bsp1286I G_dGCh'C

Cuts at:      0     373     639 
   Size:        373     266
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      46     639 
   Size:         46     593
 
BstYI r'GATC_y

Cuts at:      0      82     405     639 
   Size:         82     323     234

  Fragments arranged by size: 

                 323     234      82
 
DraI TTT'AAA

Cuts at:      0      27     639 
   Size:         27     612
 
EaeI y'GGCC_r

Cuts at:      0       2     639 
   Size:          2     637
 
EarI CTCTTCn'nnn_

Cuts at:      0     167     328     639 
   Size:        167     161     311

  Fragments arranged by size: 

                 311     167     161
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     130     162     639 
   Size:        130      32     477

  Fragments arranged by size: 

                 477     130      32
 
MscI TGG'CCA

Cuts at:      0       4     639 
   Size:          4     635
 
NsiI A_TGCA'T

Cuts at:      0     474     639 
   Size:        474     165
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     473     639 
   Size:        473     166
 
SfcI C'TryA_G

Cuts at:      0     131     639 
   Size:        131     508
 
SwaI ATTT'AAAT

Cuts at:      0      27     639 
   Size:         27     612
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      25     216     639 
   Size:         25     191     423

  Fragments arranged by size: 

                 423     191      25
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      90     122     639 
   Size:         90      32     517

  Fragments arranged by size: 

                 517      90      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     512     639 
   Size:        512     127
 

 Enzymes that do cut:

     ApoI     AseI     BbsI    BfrBI    BglII     BmrI   Bpu10I     BsaI
    BsaXI  BsiHKAI Bsp1286I    BspMI    BstYI     DraI     EaeI     EarI
    Hin4I     MscI     NsiI    PflMI     SfcI     SwaI    TaqII     TstI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI     BglI     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI