Restriction map for
pFN21AE6247
(Linear) (Six-base) MAPSORT of: KIEE6247.seq Check: 8510 from: 1 to: 636
KIEE6247.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 245 636
Size: 245 391
AhdI GACnn_n'nnGTC
Cuts at: 0 81 636
Size: 81 555
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 636
Size: 27 609
AlwNI CAG_nnn'CTG
Cuts at: 0 237 465 630 636
Size: 237 228 165 6
Fragments arranged by size:
237 228 165 6
ApaLI G'TGCA_C
Cuts at: 0 88 636
Size: 88 548
BamHI G'GATC_C
Cuts at: 0 3 636
Size: 3 633
BanI G'GyrC_C
Cuts at: 0 269 636
Size: 269 367
BanII G_rGCy'C
Cuts at: 0 176 636
Size: 176 460
BbeI G_GCGC'C
Cuts at: 0 273 636
Size: 273 363
Bme1580I G_kGCm'C
Cuts at: 0 92 434 636
Size: 92 342 202
Fragments arranged by size:
342 202 92
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 172 373 636
Size: 85 87 201 263
Fragments arranged by size:
263 201 87 85
BsaHI Gr'CG_yC
Cuts at: 0 60 270 636
Size: 60 210 366
Fragments arranged by size:
366 210 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 145 175 636
Size: 145 30 461
Fragments arranged by size:
461 145 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 372 636
Size: 372 264
BseYI C'CCAG_C
Cuts at: 0 513 636
Size: 513 123
BsiHKAI G_wGCw'C
Cuts at: 0 92 636
Size: 92 544
BsmBI CGTCTCn'nnnn_
Cuts at: 0 61 636
Size: 61 575
Bsp1286I G_dGCh'C
Cuts at: 0 92 176 434 636
Size: 92 84 258 202
Fragments arranged by size:
258 202 92 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 21 245 636
Size: 21 224 391
Fragments arranged by size:
391 224 21
BsrDI GCAATG_nn'
Cuts at: 0 500 636
Size: 500 136
BssSI C'ACGA_G
Cuts at: 0 37 616 636
Size: 37 579 20
Fragments arranged by size:
579 37 20
BstAPI GCAn_nnn'nTGC
Cuts at: 0 87 636
Size: 87 549
BstXI CCAn_nnnn'nTGG
Cuts at: 0 357 513 636
Size: 357 156 123
Fragments arranged by size:
357 156 123
BstYI r'GATC_y
Cuts at: 0 3 41 491 636
Size: 3 38 450 145
Fragments arranged by size:
450 145 38 3
BtsI GCAGTG_nn'
Cuts at: 0 367 636
Size: 367 269
EarI CTCTTCn'nnn_
Cuts at: 0 299 636
Size: 299 337
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 172 373 636
Size: 85 87 201 263
Fragments arranged by size:
263 201 87 85
EcoO109I rG'GnC_Cy
Cuts at: 0 455 636
Size: 455 181
HaeII r_GCGC'y
Cuts at: 0 273 636
Size: 273 363
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 31 63 67 99 145 177 298 330
Size: 31 32 4 32 46 32 121 32
Cuts at: 330 636
Size: 306
Fragments arranged by size:
306 121 46 32 32 32 32 31
4
HincII GTy'rAC
Cuts at: 0 210 636
Size: 210 426
KasI G'GCGC_C
Cuts at: 0 269 636
Size: 269 367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 636
Size: 548 88
MslI CAynn'nnrTG
Cuts at: 0 355 532 636
Size: 355 177 104
Fragments arranged by size:
355 177 104
NarI GG'CG_CC
Cuts at: 0 270 636
Size: 270 366
NspI r_CATG'y
Cuts at: 0 513 636
Size: 513 123
PfoI T'CCnGG_A
Cuts at: 0 560 636
Size: 560 76
PpuMI rG'GwC_Cy
Cuts at: 0 455 636
Size: 455 181
PstI C_TGCA'G
Cuts at: 0 242 374 636
Size: 242 132 262
Fragments arranged by size:
262 242 132
SapI GCTCTTCn'nnn_
Cuts at: 0 299 636
Size: 299 337
ScaI AGT'ACT
Cuts at: 0 571 636
Size: 571 65
SfcI C'TryA_G
Cuts at: 0 238 370 636
Size: 238 132 266
Fragments arranged by size:
266 238 132
SfoI GGC'GCC
Cuts at: 0 271 636
Size: 271 365
SspI AAT'ATT
Cuts at: 0 438 636
Size: 438 198
TatI w'GTAC_w
Cuts at: 0 569 636
Size: 569 67
XbaI T'CTAG_A
Cuts at: 0 141 636
Size: 141 495
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 354 636
Size: 354 282
XmnI GAAnn'nnTTC
Cuts at: 0 308 636
Size: 308 328
Enzymes that do cut:
AarI AhdI AloI AlwNI ApaLI BamHI BanI BanII
BbeI Bme1580I BpmI BsaHI BsaXI BseRI BseYI BsiHKAI
BsmBI Bsp1286I BspMI BsrDI BssSI BstAPI BstXI BstYI
BtsI EarI Eco57MI EcoO109I HaeII Hin4I HincII KasI
MmeI MslI NarI NspI PfoI PpuMI PstI SapI
ScaI SfcI SfoI SspI TatI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI ApaI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiEI
BsiWI BsmI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MscI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI StyI SwaI TaqII TstI
Tth111I XhoI XmaI ZraI