Restriction map for
pFN21AE6246
(Linear) (Six-base) MAPSORT of: KIEE6246.seq Check: 5194 from: 1 to: 801
KIEE6246.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 801
Size: 721 80
AfeI AGC'GCT
Cuts at: 0 706 801
Size: 706 95
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 46 801
Size: 46 755
ApoI r'AATT_y
Cuts at: 0 179 801
Size: 179 622
AvaI C'yCGr_G
Cuts at: 0 46 801
Size: 46 755
BanI G'GyrC_C
Cuts at: 0 238 801
Size: 238 563
BanII G_rGCy'C
Cuts at: 0 53 203 801
Size: 53 150 598
Fragments arranged by size:
598 150 53
BmgBI CAC'GTC
Cuts at: 0 95 801
Size: 95 706
BmtI G_CTAG'C
Cuts at: 0 289 801
Size: 289 512
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 459 801
Size: 459 342
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 801
Size: 215 586
BsaWI w'CCGG_w
Cuts at: 0 267 801
Size: 267 534
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 448 801
Size: 448 353
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 801
Size: 117 684
BsiEI CG_ry'CG
Cuts at: 0 62 122 801
Size: 62 60 679
Fragments arranged by size:
679 62 60
BsiHKAI G_wGCw'C
Cuts at: 0 203 801
Size: 203 598
BsmI GAATG_Cn'
Cuts at: 0 79 801
Size: 79 722
Bsp1286I G_dGCh'C
Cuts at: 0 53 203 801
Size: 53 150 598
Fragments arranged by size:
598 150 53
BspHI T'CATG_A
Cuts at: 0 308 329 416 566 638 801
Size: 308 21 87 150 72 163
Fragments arranged by size:
308 163 150 87 72 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 199 473 801
Size: 199 274 328
Fragments arranged by size:
328 274 199
BstXI CCAn_nnnn'nTGG
Cuts at: 0 520 665 801
Size: 520 145 136
Fragments arranged by size:
520 145 136
BstYI r'GATC_y
Cuts at: 0 389 801
Size: 389 412
BtgI C'CryG_G
Cuts at: 0 356 801
Size: 356 445
BtsI GCAGTG_nn'
Cuts at: 0 531 801
Size: 531 270
EaeI y'GGCC_r
Cuts at: 0 119 449 801
Size: 119 330 352
Fragments arranged by size:
352 330 119
EagI C'GGCC_G
Cuts at: 0 119 801
Size: 119 682
EarI CTCTTCn'nnn_
Cuts at: 0 365 639 801
Size: 365 274 162
Fragments arranged by size:
365 274 162
EcoICRI GAG'CTC
Cuts at: 0 201 801
Size: 201 600
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 801
Size: 721 80
HaeII r_GCGC'y
Cuts at: 0 708 801
Size: 708 93
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 752 801
Size: 752 49
MscI TGG'CCA
Cuts at: 0 451 801
Size: 451 350
MslI CAynn'nnrTG
Cuts at: 0 518 801
Size: 518 283
MspA1I CmG'CkG
Cuts at: 0 210 801
Size: 210 591
NcoI C'CATG_G
Cuts at: 0 356 801
Size: 356 445
NheI G'CTAG_C
Cuts at: 0 285 801
Size: 285 516
PstI C_TGCA'G
Cuts at: 0 209 620 801
Size: 209 411 181
Fragments arranged by size:
411 209 181
PvuII CAG'CTG
Cuts at: 0 210 801
Size: 210 591
SacI G_AGCT'C
Cuts at: 0 203 801
Size: 203 598
SfcI C'TryA_G
Cuts at: 0 205 616 801
Size: 205 411 185
Fragments arranged by size:
411 205 185
SmaI CCC'GGG
Cuts at: 0 48 801
Size: 48 753
SmlI C'TyrA_G
Cuts at: 0 230 801
Size: 230 571
StyI C'CwwG_G
Cuts at: 0 356 801
Size: 356 445
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 517 685 801
Size: 517 168 116
Fragments arranged by size:
517 168 116
XmaI C'CCGG_G
Cuts at: 0 46 801
Size: 46 755
Enzymes that do cut:
AcuI AfeI AlfI ApoI AvaI BanI BanII BmgBI
BmtI BplI BpuEI BsaWI BseRI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BstXI BstYI BtgI BtsI
EaeI EagI EarI EcoICRI Eco57MI HaeII MmeI MscI
MslI MspA1I NcoI NheI PstI PvuII SacI SfcI
SmaI SmlI StyI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseYI
BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EciI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmnI ZraI