Restriction map for
pFN21AE6243
(Linear) (Six-base) MAPSORT of: KIEE6243.seq Check: 389 from: 1 to: 903
KIEE6243.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
Acc65I G'GTAC_C
Cuts at: 0 451 903
Size: 451 452
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 903
Size: 413 490
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 435 468 903
Size: 435 33 435
Fragments arranged by size:
435 435 33
BamHI G'GATC_C
Cuts at: 0 491 903
Size: 491 412
BanI G'GyrC_C
Cuts at: 0 451 903
Size: 451 452
BanII G_rGCy'C
Cuts at: 0 620 903
Size: 620 283
BbsI GAAGACnn'nnnn_
Cuts at: 0 187 903
Size: 187 716
BbvCI CC'TCA_GC
Cuts at: 0 757 903
Size: 757 146
BglI GCCn_nnn'nGGC
Cuts at: 0 841 903
Size: 841 62
BlpI GC'TnA_GC
Cuts at: 0 322 903
Size: 322 581
Bme1580I G_kGCm'C
Cuts at: 0 627 903
Size: 627 276
BmrI ACTGGGnnnn_n'
Cuts at: 0 678 903
Size: 678 225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 903
Size: 410 493
Bpu10I CC'TnA_GC
Cuts at: 0 757 903
Size: 757 146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 682 903
Size: 16 666 221
Fragments arranged by size:
666 221 16
BsaWI w'CCGG_w
Cuts at: 0 176 594 903
Size: 176 418 309
Fragments arranged by size:
418 309 176
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 705 735 903
Size: 705 30 168
Fragments arranged by size:
705 168 30
BseYI C'CCAG_C
Cuts at: 0 332 346 540 643 831 903
Size: 332 14 194 103 188 72
Fragments arranged by size:
332 194 188 103 72 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 903
Size: 701 202
BsiHKAI G_wGCw'C
Cuts at: 0 323 620 698 903
Size: 323 297 78 205
Fragments arranged by size:
323 297 205 78
Bsp1286I G_dGCh'C
Cuts at: 0 323 620 627 698 903
Size: 323 297 7 71 205
Fragments arranged by size:
323 297 205 71 7
BspEI T'CCGG_A
Cuts at: 0 176 594 903
Size: 176 418 309
Fragments arranged by size:
418 309 176
BspHI T'CATG_A
Cuts at: 0 380 560 903
Size: 380 180 343
Fragments arranged by size:
380 343 180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 47 903
Size: 47 856
BsrBI CCG'CTC
Cuts at: 0 30 903
Size: 30 873
BsrDI GCAATG_nn'
Cuts at: 0 650 903
Size: 650 253
BsrFI r'CCGG_y
Cuts at: 0 46 903
Size: 46 857
BsrGI T'GTAC_A
Cuts at: 0 794 903
Size: 794 109
BstEII G'GTnAC_C
Cuts at: 0 82 903
Size: 82 821
BstXI CCAn_nnnn'nTGG
Cuts at: 0 487 903
Size: 487 416
BstYI r'GATC_y
Cuts at: 0 491 632 659 903
Size: 491 141 27 244
Fragments arranged by size:
491 244 141 27
BtsI GCAGTG_nn'
Cuts at: 0 818 903
Size: 818 85
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 113 148 903
Size: 113 35 755
Fragments arranged by size:
755 113 35
EaeI y'GGCC_r
Cuts at: 0 2 44 903
Size: 2 42 859
Fragments arranged by size:
859 42 2
EcoICRI GAG'CTC
Cuts at: 0 618 903
Size: 618 285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 413 903
Size: 410 3 490
Fragments arranged by size:
490 410 3
EcoO109I rG'GnC_Cy
Cuts at: 0 161 903
Size: 161 742
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 185 217 383 415 705 737 903
Size: 185 32 166 32 290 32 166
Fragments arranged by size:
290 185 166 166 32 32 32
KpnI G_GTAC'C
Cuts at: 0 455 903
Size: 455 448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 903
Size: 132 771
MslI CAynn'nnrTG
Cuts at: 0 250 570 903
Size: 250 320 333
Fragments arranged by size:
333 320 250
MspA1I CmG'CkG
Cuts at: 0 346 903
Size: 346 557
NaeI GCC'GGC
Cuts at: 0 48 903
Size: 48 855
NdeI CA'TA_TG
Cuts at: 0 567 903
Size: 567 336
NgoMIV G'CCGG_C
Cuts at: 0 46 903
Size: 46 857
PflMI CCAn_nnn'nTGG
Cuts at: 0 547 903
Size: 547 356
PfoI T'CCnGG_A
Cuts at: 0 387 770 903
Size: 387 383 133
Fragments arranged by size:
387 383 133
PpuMI rG'GwC_Cy
Cuts at: 0 161 903
Size: 161 742
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 787 819 903
Size: 787 32 84
Fragments arranged by size:
787 84 32
PstI C_TGCA'G
Cuts at: 0 116 903
Size: 116 787
PvuII CAG'CTG
Cuts at: 0 346 903
Size: 346 557
SacI G_AGCT'C
Cuts at: 0 620 903
Size: 620 283
SfcI C'TryA_G
Cuts at: 0 112 903
Size: 112 791
SmlI C'TyrA_G
Cuts at: 0 31 697 903
Size: 31 666 206
Fragments arranged by size:
666 206 31
StyI C'CwwG_G
Cuts at: 0 455 852 903
Size: 455 397 51
Fragments arranged by size:
455 397 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 70 323 903
Size: 70 253 580
Fragments arranged by size:
580 253 70
TatI w'GTAC_w
Cuts at: 0 152 443 794 903
Size: 152 291 351 109
Fragments arranged by size:
351 291 152 109
Tth111I GACn'n_nGTC
Cuts at: 0 97 903
Size: 97 806
Enzymes that do cut:
Acc65I AcuI BaeI BamHI BanI BanII BbsI BbvCI
BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaWI
BsaXI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BstEII BstXI BstYI BtsI
CspCI EaeI EcoICRI Eco57MI EcoO109I Hin4I KpnI MmeI
MslI MspA1I NaeI NdeI NgoMIV PflMI PfoI PpuMI
PsrI PstI PvuII SacI SfcI SmlI StyI TaqII
TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BbeI BcgI BciVI
BclI BfrBI BglII BmgBI BmtI BplI BsaI BsaAI
BsaBI BsaHI BseRI BsiEI BsiWI BsmI BsmBI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I BtgI BtgZI ClaI
DraI DraIII DrdI EagI EarI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI MfeI MluI MscI NarI NcoI NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI XbaI XcmI XhoI XmaI
XmnI ZraI