Restriction map for
pFN21AE6241
(Linear) (Six-base) MAPSORT of: KIEE6241.seq Check: 2574 from: 1 to: 828
KIEE6241.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 752 828
Size: 752 76
AfeI AGC'GCT
Cuts at: 0 172 828
Size: 172 656
AleI CACnn'nnGTG
Cuts at: 0 292 828
Size: 292 536
ApoI r'AATT_y
Cuts at: 0 590 724 828
Size: 590 134 104
Fragments arranged by size:
590 134 104
BamHI G'GATC_C
Cuts at: 0 56 828
Size: 56 772
BanI G'GyrC_C
Cuts at: 0 133 426 525 828
Size: 133 293 99 303
Fragments arranged by size:
303 293 133 99
BanII G_rGCy'C
Cuts at: 0 11 189 229 828
Size: 11 178 40 599
Fragments arranged by size:
599 178 40 11
BbvCI CC'TCA_GC
Cuts at: 0 503 828
Size: 503 325
BglI GCCn_nnn'nGGC
Cuts at: 0 213 216 828
Size: 213 3 612
Fragments arranged by size:
612 213 3
Bme1580I G_kGCm'C
Cuts at: 0 803 828
Size: 803 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 828
Size: 469 359
Bpu10I CC'TnA_GC
Cuts at: 0 503 828
Size: 503 325
BsaI GGTCTCn'nnnn_
Cuts at: 0 74 828
Size: 74 754
BsaAI yAC'GTr
Cuts at: 0 294 828
Size: 294 534
BsaHI Gr'CG_yC
Cuts at: 0 15 325 828
Size: 15 310 503
Fragments arranged by size:
503 310 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 268 271 828
Size: 268 3 557
Fragments arranged by size:
557 268 3
BseYI C'CCAG_C
Cuts at: 0 318 828
Size: 318 510
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 828
Size: 524 304
BsiHKAI G_wGCw'C
Cuts at: 0 229 828
Size: 229 599
Bsp1286I G_dGCh'C
Cuts at: 0 11 189 229 803 828
Size: 11 178 40 574 25
Fragments arranged by size:
574 178 40 25 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 385 828
Size: 385 443
BsrBI CCG'CTC
Cuts at: 0 261 828
Size: 261 567
BsrDI GCAATG_nn'
Cuts at: 0 199 828
Size: 199 629
BssHII G'CGCG_C
Cuts at: 0 245 828
Size: 245 583
BstAPI GCAn_nnn'nTGC
Cuts at: 0 400 807 828
Size: 400 407 21
Fragments arranged by size:
407 400 21
BstXI CCAn_nnnn'nTGG
Cuts at: 0 370 828
Size: 370 458
BstYI r'GATC_y
Cuts at: 0 56 735 828
Size: 56 679 93
Fragments arranged by size:
679 93 56
BtgI C'CryG_G
Cuts at: 0 211 308 828
Size: 211 97 520
Fragments arranged by size:
520 211 97
EaeI y'GGCC_r
Cuts at: 0 296 828
Size: 296 532
EcoICRI GAG'CTC
Cuts at: 0 227 828
Size: 227 601
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 752 828
Size: 469 283 76
Fragments arranged by size:
469 283 76
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 775 828
Size: 775 53
HaeII r_GCGC'y
Cuts at: 0 54 174 828
Size: 54 120 654
Fragments arranged by size:
654 120 54
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 828
Size: 97 731
MslI CAynn'nnrTG
Cuts at: 0 292 828
Size: 292 536
MspA1I CmG'CkG
Cuts at: 0 213 301 828
Size: 213 88 527
Fragments arranged by size:
527 213 88
NspI r_CATG'y
Cuts at: 0 318 478 828
Size: 318 160 350
Fragments arranged by size:
350 318 160
PasI CC'CwG_GG
Cuts at: 0 64 828
Size: 64 764
PflMI CCAn_nnn'nTGG
Cuts at: 0 586 828
Size: 586 242
PfoI T'CCnGG_A
Cuts at: 0 18 816 828
Size: 18 798 12
Fragments arranged by size:
798 18 12
PstI C_TGCA'G
Cuts at: 0 87 178 828
Size: 87 91 650
Fragments arranged by size:
650 91 87
SacI G_AGCT'C
Cuts at: 0 229 828
Size: 229 599
SacII CC_GC'GG
Cuts at: 0 214 828
Size: 214 614
SfcI C'TryA_G
Cuts at: 0 83 174 828
Size: 83 91 654
Fragments arranged by size:
654 91 83
SphI G_CATG'C
Cuts at: 0 318 478 828
Size: 318 160 350
Fragments arranged by size:
350 318 160
SspI AAT'ATT
Cuts at: 0 606 828
Size: 606 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 117 149 828
Size: 117 32 679
Fragments arranged by size:
679 117 32
XmnI GAAnn'nnTTC
Cuts at: 0 235 828
Size: 235 593
Enzymes that do cut:
AcuI AfeI AleI ApoI BamHI BanI BanII BbvCI
BglI Bme1580I BpmI Bpu10I BsaI BsaAI BsaHI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BssHII
BstAPI BstXI BstYI BtgI EaeI EcoICRI Eco57MI FalI
HaeII MmeI MslI MspA1I NspI PasI PflMI PfoI
PstI SacI SacII SfcI SphI SspI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
BpuEI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrFI BsrGI BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoNI EcoO109I EcoRI EcoRV FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI StuI StyI SwaI TaqII TatI Tth111I XbaI
XcmI XhoI XmaI ZraI