Restriction map for
pFN21AE6240
(Linear) (Six-base) MAPSORT of: KIEE6240.seq Check: 7026 from: 1 to: 585
KIEE6240.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 532 585
Size: 424 108 53
Fragments arranged by size:
424 108 53
AhdI GACnn_n'nnGTC
Cuts at: 0 468 585
Size: 468 117
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 136 585
Size: 136 449
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 460 492 585
Size: 460 32 93
Fragments arranged by size:
460 93 32
ApaI G_GGCC'C
Cuts at: 0 326 585
Size: 326 259
ApoI r'AATT_y
Cuts at: 0 313 585
Size: 313 272
AvaI C'yCGr_G
Cuts at: 0 325 585
Size: 325 260
BanI G'GyrC_C
Cuts at: 0 28 585
Size: 28 557
BanII G_rGCy'C
Cuts at: 0 326 530 585
Size: 326 204 55
Fragments arranged by size:
326 204 55
BbeI G_GCGC'C
Cuts at: 0 32 585
Size: 32 553
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 273 307 585
Size: 273 34 278
Fragments arranged by size:
278 273 34
BfrBI ATG'CAT
Cuts at: 0 361 585
Size: 361 224
BglI GCCn_nnn'nGGC
Cuts at: 0 330 585
Size: 330 255
Bme1580I G_kGCm'C
Cuts at: 0 326 573 585
Size: 326 247 12
Fragments arranged by size:
326 247 12
BmgBI CAC'GTC
Cuts at: 0 80 585
Size: 80 505
BmrI ACTGGGnnnn_n'
Cuts at: 0 474 585
Size: 474 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 585
Size: 67 518
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 585
Size: 187 398
BsaAI yAC'GTr
Cuts at: 0 498 585
Size: 498 87
BsaHI Gr'CG_yC
Cuts at: 0 29 248 585
Size: 29 219 337
Fragments arranged by size:
337 219 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 460 490 585
Size: 460 30 95
Fragments arranged by size:
460 95 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 538 585
Size: 538 47
BseYI C'CCAG_C
Cuts at: 0 443 585
Size: 443 142
Bsp1286I G_dGCh'C
Cuts at: 0 326 530 573 585
Size: 326 204 43 12
Fragments arranged by size:
326 204 43 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 101 585
Size: 101 484
BsrBI CCG'CTC
Cuts at: 0 53 87 585
Size: 53 34 498
Fragments arranged by size:
498 53 34
BsrFI r'CCGG_y
Cuts at: 0 150 585
Size: 150 435
BtsI GCAGTG_nn'
Cuts at: 0 434 585
Size: 434 151
EaeI y'GGCC_r
Cuts at: 0 155 272 585
Size: 155 117 313
Fragments arranged by size:
313 155 117
EarI CTCTTCn'nnn_
Cuts at: 0 348 585
Size: 348 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 424 532 585
Size: 67 357 108 53
Fragments arranged by size:
357 108 67 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 391 585
Size: 391 194
EcoO109I rG'GnC_Cy
Cuts at: 0 255 369 585
Size: 255 114 216
Fragments arranged by size:
255 216 114
HaeII r_GCGC'y
Cuts at: 0 32 240 585
Size: 32 208 345
Fragments arranged by size:
345 208 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 454 486 585
Size: 454 32 99
Fragments arranged by size:
454 99 32
KasI G'GCGC_C
Cuts at: 0 28 585
Size: 28 557
MscI TGG'CCA
Cuts at: 0 274 585
Size: 274 311
MslI CAynn'nnrTG
Cuts at: 0 268 585
Size: 268 317
MspA1I CmG'CkG
Cuts at: 0 161 585
Size: 161 424
NaeI GCC'GGC
Cuts at: 0 152 585
Size: 152 433
NarI GG'CG_CC
Cuts at: 0 29 585
Size: 29 556
NgoMIV G'CCGG_C
Cuts at: 0 150 585
Size: 150 435
NsiI A_TGCA'T
Cuts at: 0 363 585
Size: 363 222
PfoI T'CCnGG_A
Cuts at: 0 251 585
Size: 251 334
PmlI CAC'GTG
Cuts at: 0 498 585
Size: 498 87
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 460 492 585
Size: 460 32 93
Fragments arranged by size:
460 93 32
PpuMI rG'GwC_Cy
Cuts at: 0 255 585
Size: 255 330
PspOMI G'GGCC_C
Cuts at: 0 322 585
Size: 322 263
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 330 585
Size: 330 255
SfoI GGC'GCC
Cuts at: 0 30 585
Size: 30 555
SmlI C'TyrA_G
Cuts at: 0 202 585
Size: 202 383
TatI w'GTAC_w
Cuts at: 0 536 585
Size: 536 49
Tth111I GACn'n_nGTC
Cuts at: 0 247 585
Size: 247 338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 384 585
Size: 384 201
Enzymes that do cut:
AcuI AhdI AlfI AloI ApaI ApoI AvaI BanI
BanII BbeI BcgI BfrBI BglI Bme1580I BmgBI BmrI
BpmI BpuEI BsaAI BsaHI BsaXI BseRI BseYI Bsp1286I
BspMI BsrBI BsrFI BtsI EaeI EarI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI PfoI PmlI PpiI PpuMI PspOMI
SfiI SfoI SmlI TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BciVI BclI BglII BlpI
BmtI BplI Bpu10I BsaI BsaBI BsaWI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
NcoI NdeI NheI NotI NruI NspI PacI PasI
PciI PflMI PmeI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TstI XbaI XhoI XmaI XmnI ZraI