Restriction map for
pFN21AE6238
(Linear) (Six-base) MAPSORT of: KIEE6238.seq Check: 7691 from: 1 to: 909
KIEE6238.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AvaI C'yCGr_G
Cuts at: 0 374 909
Size: 374 535
BanII G_rGCy'C
Cuts at: 0 34 909
Size: 34 875
BbsI GAAGACnn'nnnn_
Cuts at: 0 883 909
Size: 883 26
BclI T'GATC_A
Cuts at: 0 428 909
Size: 428 481
BglII A'GATC_T
Cuts at: 0 439 909
Size: 439 470
BlpI GC'TnA_GC
Cuts at: 0 173 909
Size: 173 736
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 680 909
Size: 680 229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 909
Size: 650 259
BsaI GGTCTCn'nnnn_
Cuts at: 0 43 163 909
Size: 43 120 746
Fragments arranged by size:
746 120 43
BsaAI yAC'GTr
Cuts at: 0 69 909
Size: 69 840
BsaWI w'CCGG_w
Cuts at: 0 34 857 909
Size: 34 823 52
Fragments arranged by size:
823 52 34
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 319 909
Size: 319 590
BseYI C'CCAG_C
Cuts at: 0 53 909
Size: 53 856
BsiHKAI G_wGCw'C
Cuts at: 0 26 80 909
Size: 26 54 829
Fragments arranged by size:
829 54 26
BsmI GAATG_Cn'
Cuts at: 0 521 909
Size: 521 388
Bsp1286I G_dGCh'C
Cuts at: 0 26 34 80 909
Size: 26 8 46 829
Fragments arranged by size:
829 46 26 8
BspEI T'CCGG_A
Cuts at: 0 857 909
Size: 857 52
BsrBI CCG'CTC
Cuts at: 0 60 909
Size: 60 849
BsrDI GCAATG_nn'
Cuts at: 0 179 909
Size: 179 730
BsrGI T'GTAC_A
Cuts at: 0 324 909
Size: 324 585
BstXI CCAn_nnnn'nTGG
Cuts at: 0 729 909
Size: 729 180
BstYI r'GATC_y
Cuts at: 0 439 909
Size: 439 470
BtgI C'CryG_G
Cuts at: 0 261 909
Size: 261 648
ClaI AT'CG_AT
Cuts at: 0 191 909
Size: 191 718
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 459 494 726 761 909
Size: 459 35 232 35 148
Fragments arranged by size:
459 232 148 35 35
EaeI y'GGCC_r
Cuts at: 0 11 909
Size: 11 898
EarI CTCTTCn'nnn_
Cuts at: 0 107 509 649 909
Size: 107 402 140 260
Fragments arranged by size:
402 260 140 107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 909
Size: 19 890
EcoO109I rG'GnC_Cy
Cuts at: 0 313 864 909
Size: 313 551 45
Fragments arranged by size:
551 313 45
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 885 909
Size: 885 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 368 372 400 404 909
Size: 368 4 28 4 505
Fragments arranged by size:
505 368 28 4 4
HincII GTy'rAC
Cuts at: 0 698 738 909
Size: 698 40 171
Fragments arranged by size:
698 171 40
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 909
Size: 133 776
MslI CAynn'nnrTG
Cuts at: 0 67 85 909
Size: 67 18 824
Fragments arranged by size:
824 67 18
NcoI C'CATG_G
Cuts at: 0 261 909
Size: 261 648
NspI r_CATG'y
Cuts at: 0 813 909
Size: 813 96
PpuMI rG'GwC_Cy
Cuts at: 0 313 864 909
Size: 313 551 45
Fragments arranged by size:
551 313 45
ScaI AGT'ACT
Cuts at: 0 475 909
Size: 475 434
SmlI C'TyrA_G
Cuts at: 0 374 665 909
Size: 374 291 244
Fragments arranged by size:
374 291 244
SphI G_CATG'C
Cuts at: 0 813 909
Size: 813 96
SspI AAT'ATT
Cuts at: 0 126 909
Size: 126 783
StyI C'CwwG_G
Cuts at: 0 246 261 909
Size: 246 15 648
Fragments arranged by size:
648 246 15
TatI w'GTAC_w
Cuts at: 0 324 473 909
Size: 324 149 436
Fragments arranged by size:
436 324 149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 404 436 909
Size: 404 32 473
Fragments arranged by size:
473 404 32
XhoI C'TCGA_G
Cuts at: 0 374 909
Size: 374 535
Enzymes that do cut:
AvaI BanII BbsI BclI BglII BlpI BplI BpuEI
BsaI BsaAI BsaWI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspEI BsrBI BsrDI BsrGI BstXI BstYI BtgI ClaI
CspCI EaeI EarI EciI EcoO109I FalI Hin4I HincII
MmeI MslI NcoI NspI PpuMI ScaI SmlI SphI
SspI StyI TatI TstI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BciVI BfrBI BglI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BsaBI BsaHI BsaXI
BsgI BsiEI BsiWI BsmBI BspHI BspMI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI
DraI DraIII DrdI EagI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MscI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI
TaqII Tth111I XbaI XcmI XmaI XmnI ZraI