Restriction map for
pFN21AE6236
(Linear) (Six-base) MAPSORT of: KIEE6236.seq Check: 5931 from: 1 to: 717
KIEE6236.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 633 717
Size: 633 84
AleI CACnn'nnGTG
Cuts at: 0 13 127 717
Size: 13 114 590
Fragments arranged by size:
590 114 13
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 31 717
Size: 31 686
AlwNI CAG_nnn'CTG
Cuts at: 0 24 489 717
Size: 24 465 228
Fragments arranged by size:
465 228 24
ApaI G_GGCC'C
Cuts at: 0 666 717
Size: 666 51
ApaLI G'TGCA_C
Cuts at: 0 309 717
Size: 309 408
AvaI C'yCGr_G
Cuts at: 0 270 717
Size: 270 447
BanI G'GyrC_C
Cuts at: 0 109 364 532 564 717
Size: 109 255 168 32 153
Fragments arranged by size:
255 168 153 109 32
BanII G_rGCy'C
Cuts at: 0 149 666 717
Size: 149 517 51
Fragments arranged by size:
517 149 51
BclI T'GATC_A
Cuts at: 0 65 717
Size: 65 652
BglI GCCn_nnn'nGGC
Cuts at: 0 10 87 717
Size: 10 77 630
Fragments arranged by size:
630 77 10
BglII A'GATC_T
Cuts at: 0 194 717
Size: 194 523
BlpI GC'TnA_GC
Cuts at: 0 37 717
Size: 37 680
Bme1580I G_kGCm'C
Cuts at: 0 61 112 313 331 666 717
Size: 61 51 201 18 335 51
Fragments arranged by size:
335 201 61 51 51 18
BmgBI CAC'GTC
Cuts at: 0 426 717
Size: 426 291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 717
Size: 214 503
BsaAI yAC'GTr
Cuts at: 0 105 714 717
Size: 105 609 3
Fragments arranged by size:
609 105 3
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 148 178 717
Size: 148 30 539
Fragments arranged by size:
539 148 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 498 717
Size: 498 219
BseYI C'CCAG_C
Cuts at: 0 457 717
Size: 457 260
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 451 717
Size: 37 414 266
Fragments arranged by size:
414 266 37
BsiEI CG_ry'CG
Cuts at: 0 14 128 717
Size: 14 114 589
Fragments arranged by size:
589 114 14
BsiHKAI G_wGCw'C
Cuts at: 0 313 717
Size: 313 404
BsmBI CGTCTCn'nnnn_
Cuts at: 0 411 717
Size: 411 306
Bsp1286I G_dGCh'C
Cuts at: 0 61 112 149 313 331 666 717
Size: 61 51 37 164 18 335 51
Fragments arranged by size:
335 164 61 51 51 37 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 633 717
Size: 633 84
BsrFI r'CCGG_y
Cuts at: 0 382 717
Size: 382 335
BstAPI GCAn_nnn'nTGC
Cuts at: 0 281 317 717
Size: 281 36 400
Fragments arranged by size:
400 281 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 426 717
Size: 426 291
BstYI r'GATC_y
Cuts at: 0 194 717
Size: 194 523
BtsI GCAGTG_nn'
Cuts at: 0 608 717
Size: 608 109
EaeI y'GGCC_r
Cuts at: 0 2 11 125 380 401 480 680 717
Size: 2 9 114 255 21 79 200 37
Fragments arranged by size:
255 200 114 79 37 21 9 2
EagI C'GGCC_G
Cuts at: 0 11 125 717
Size: 11 114 592
Fragments arranged by size:
592 114 11
EarI CTCTTCn'nnn_
Cuts at: 0 376 717
Size: 376 341
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 105 717
Size: 105 612
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 717
Size: 214 503
EcoO109I rG'GnC_Cy
Cuts at: 0 486 575 585 663 717
Size: 486 89 10 78 54
Fragments arranged by size:
486 89 78 54 10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 363 717
Size: 363 354
FseI GG_CCGG'CC
Cuts at: 0 386 717
Size: 386 331
HaeII r_GCGC'y
Cuts at: 0 217 717
Size: 217 500
MscI TGG'CCA
Cuts at: 0 4 482 717
Size: 4 478 235
Fragments arranged by size:
478 235 4
MslI CAynn'nnrTG
Cuts at: 0 13 127 301 717
Size: 13 114 174 416
Fragments arranged by size:
416 174 114 13
MspA1I CmG'CkG
Cuts at: 0 450 461 643 692 717
Size: 450 11 182 49 25
Fragments arranged by size:
450 182 49 25 11
NaeI GCC'GGC
Cuts at: 0 384 717
Size: 384 333
NgoMIV G'CCGG_C
Cuts at: 0 382 717
Size: 382 335
PasI CC'CwG_GG
Cuts at: 0 593 717
Size: 593 124
PfoI T'CCnGG_A
Cuts at: 0 409 717
Size: 409 308
PmlI CAC'GTG
Cuts at: 0 105 717
Size: 105 612
PspOMI G'GGCC_C
Cuts at: 0 662 717
Size: 662 55
PvuII CAG'CTG
Cuts at: 0 643 717
Size: 643 74
SfcI C'TryA_G
Cuts at: 0 570 717
Size: 570 147
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 10 717
Size: 10 707
StyI C'CwwG_G
Cuts at: 0 323 717
Size: 323 394
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 144 148 176 180 717
Size: 144 4 28 4 537
Fragments arranged by size:
537 144 28 4 4
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 423 717
Size: 423 294
Enzymes that do cut:
AarI AleI AlfI AlwNI ApaI ApaLI AvaI BanI
BanII BclI BglI BglII BlpI Bme1580I BmgBI BpmI
BsaAI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrFI BstAPI BstXI BstYI BtsI EaeI
EagI EarI EciI Eco57MI EcoO109I FalI FseI HaeII
MscI MslI MspA1I NaeI NgoMIV PasI PfoI PmlI
PspOMI PvuII SfcI SfiI StyI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BfrBI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI
EcoRV FspI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI Tth111I
XbaI XhoI XmaI XmnI ZraI