(Linear) (Six-base) MAPSORT of: KIEE6231.seq  Check: 6816  from: 1  to: 744



KIEE6231.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     744 
   Size:        130     614
 
AleI CACnn'nnGTG

Cuts at:      0     441     610     744 
   Size:        441     169     134

  Fragments arranged by size: 

                 441     169     134
 
ApoI r'AATT_y

Cuts at:      0     219     586     744 
   Size:        219     367     158

  Fragments arranged by size: 

                 367     219     158
 
BamHI G'GATC_C

Cuts at:      0     550     744 
   Size:        550     194
 
BglI GCCn_nnn'nGGC

Cuts at:      0     544     744 
   Size:        544     200
 
BglII A'GATC_T

Cuts at:      0     155     744 
   Size:        155     589
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      19     744 
   Size:         19     725
 
BsiHKAI G_wGCw'C

Cuts at:      0      41     375     605     744 
   Size:         41     334     230     139

  Fragments arranged by size: 

                 334     230     139      41
 
BsmI GAATG_Cn'

Cuts at:      0     362     744 
   Size:        362     382
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     375     605     744 
   Size:         41     334     230     139

  Fragments arranged by size: 

                 334     230     139      41
 
BspHI T'CATG_A

Cuts at:      0     498     711     744 
   Size:        498     213      33

  Fragments arranged by size: 

                 498     213      33
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     336     744 
   Size:        336     408
 
BstYI r'GATC_y

Cuts at:      0     155     550     744 
   Size:        155     395     194

  Fragments arranged by size: 

                 395     194     155
 
BtgI C'CryG_G

Cuts at:      0     515     744 
   Size:        515     229
 
BtsI GCAGTG_nn'

Cuts at:      0     436     448     605     744 
   Size:        436      12     157     139

  Fragments arranged by size: 

                 436     157     139      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     744 
   Size:        130     614
 
EcoO109I rG'GnC_Cy

Cuts at:      0     271     744 
   Size:        271     473
 
EcoRI G'AATT_C

Cuts at:      0     586     744 
   Size:        586     158
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     561     593     744 
   Size:        561      32     151

  Fragments arranged by size: 

                 561     151      32
 
HincII GTy'rAC

Cuts at:      0     326     744 
   Size:        326     418
 
HindIII A'AGCT_T

Cuts at:      0     331     744 
   Size:        331     413
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     336     431     744 
   Size:        336      95     313

  Fragments arranged by size: 

                 336     313      95
 
MslI CAynn'nnrTG

Cuts at:      0      34     441     610     716     744 
   Size:         34     407     169     106      28

  Fragments arranged by size: 

                 407     169     106      34      28
 
NspI r_CATG'y

Cuts at:      0     696     744 
   Size:        696      48
 
PasI CC'CwG_GG

Cuts at:      0     646     744 
   Size:        646      98
 
PpuMI rG'GwC_Cy

Cuts at:      0     271     744 
   Size:        271     473
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     241     273     744 
   Size:        241      32     471

  Fragments arranged by size: 

                 471     241      32
 
PstI C_TGCA'G

Cuts at:      0     443     744 
   Size:        443     301
 
SfcI C'TryA_G

Cuts at:      0     439     744 
   Size:        439     305
 
TatI w'GTAC_w

Cuts at:      0     654     744 
   Size:        654      90
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     411     443     744 
   Size:        411      32     301

  Fragments arranged by size: 

                 411     301      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     160     540     744 
   Size:        160     380     204

  Fragments arranged by size: 

                 380     204     160
 

 Enzymes that do cut:

     AcuI     AleI     ApoI    BamHI     BglI    BglII     BsaI  BsiHKAI
     BsmI Bsp1286I    BspHI    BstXI    BstYI     BtgI     BtsI  Eco57MI
 EcoO109I    EcoRI    Hin4I   HincII  HindIII     MmeI     MslI     NspI
     PasI    PpuMI     PsrI     PstI     SfcI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI