(Linear) (Six-base) MAPSORT of: KIEE6229.seq  Check: 9699  from: 1  to: 579



KIEE6229.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

AccI GT'mk_AC

Cuts at:      0     338     579 
   Size:        338     241
 
AclI AA'CG_TT

Cuts at:      0     208     579 
   Size:        208     371
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     221     579 
   Size:        221     358
 
AseI AT'TA_AT

Cuts at:      0     163     579 
   Size:        163     416
 
BanII G_rGCy'C

Cuts at:      0     471     579 
   Size:        471     108
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     213     579 
   Size:        213     366
 
BclI T'GATC_A

Cuts at:      0       2     579 
   Size:          2     577
 
BglII A'GATC_T

Cuts at:      0     261     579 
   Size:        261     318
 
BlpI GC'TnA_GC

Cuts at:      0     459     579 
   Size:        459     120
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     455     579 
   Size:        455     124
 
BsaBI GATnn'nnATC

Cuts at:      0      16     579 
   Size:         16     563
 
BsiHKAI G_wGCw'C

Cuts at:      0     471     579 
   Size:        471     108
 
Bsp1286I G_dGCh'C

Cuts at:      0     471     579 
   Size:        471     108
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     572     579 
   Size:        572       7
 
BstYI r'GATC_y

Cuts at:      0     172     261     515     579 
   Size:        172      89     254      64

  Fragments arranged by size: 

                 254     172      89      64
 
BtgI C'CryG_G

Cuts at:      0      30      40     579 
   Size:         30      10     539

  Fragments arranged by size: 

                 539      30      10
 
DraI TTT'AAA

Cuts at:      0     576     579 
   Size:        576       3
 
EcoICRI GAG'CTC

Cuts at:      0     469     579 
   Size:        469     110
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     221     455     579 
   Size:        221     234     124

  Fragments arranged by size: 

                 234     221     124
 
MspA1I CmG'CkG

Cuts at:      0      42     490     579 
   Size:         42     448      89

  Fragments arranged by size: 

                 448      89      42
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     329     361     579 
   Size:        329      32     218

  Fragments arranged by size: 

                 329     218      32
 
PvuII CAG'CTG

Cuts at:      0     490     579 
   Size:        490      89
 
SacI G_AGCT'C

Cuts at:      0     471     579 
   Size:        471     108
 
SacII CC_GC'GG

Cuts at:      0      43     579 
   Size:         43     536
 
SfcI C'TryA_G

Cuts at:      0      66     579 
   Size:         66     513
 
SspI AAT'ATT

Cuts at:      0     354     528     579 
   Size:        354     174      51

  Fragments arranged by size: 

                 354     174      51
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AseI    BanII     BbsI     BclI    BglII
     BlpI     BpmI    BsaBI  BsiHKAI Bsp1286I    BspMI    BstYI     BtgI
     DraI  EcoICRI  Eco57MI   MspA1I     PsrI    PvuII     SacI    SacII
     SfcI     SspI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PstI     PvuI    RsrII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI