(Linear) (Six-base) MAPSORT of: KIEE6226.seq  Check: 1801  from: 1  to: 633



KIEE6226.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

ApoI r'AATT_y

Cuts at:      0     353     607     633 
   Size:        353     254      26

  Fragments arranged by size: 

                 353     254      26
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     584     633 
   Size:        584      49
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     265     415     633 
   Size:         79     186     150     218

  Fragments arranged by size: 

                 218     186     150      79
 
BspHI T'CATG_A

Cuts at:      0     537     633 
   Size:        537      96
 
BstBI TT'CG_AA

Cuts at:      0     402     633 
   Size:        402     231
 
BstYI r'GATC_y

Cuts at:      0     415     441     633 
   Size:        415      26     192

  Fragments arranged by size: 

                 415     192      26
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     100     633 
   Size:        100     533
 
DraI TTT'AAA

Cuts at:      0     491     633 
   Size:        491     142
 
EarI CTCTTCn'nnn_

Cuts at:      0     292     633 
   Size:        292     341
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     265     415     633 
   Size:         79     186     150     218

  Fragments arranged by size: 

                 218     186     150      79
 
MslI CAynn'nnrTG

Cuts at:      0     209     539     633 
   Size:        209     330      94

  Fragments arranged by size: 

                 330     209      94
 
PasI CC'CwG_GG

Cuts at:      0     234     633 
   Size:        234     399
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     438     633 
   Size:        438     195
 
PfoI T'CCnGG_A

Cuts at:      0     242     392     633 
   Size:        242     150     241

  Fragments arranged by size: 

                 242     241     150
 
SspI AAT'ATT

Cuts at:      0     384     633 
   Size:        384     249
 
TatI w'GTAC_w

Cuts at:      0     367     633 
   Size:        367     266
 
XmnI GAAnn'nnTTC

Cuts at:      0     103     633 
   Size:        103     530
 

 Enzymes that do cut:

     ApoI     BbsI     BpmI    BspHI    BstBI    BstYI    BtgZI     DraI
     EarI  Eco57MI     MslI     PasI    PflMI     PfoI     SspI     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     BtsI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI