(Linear) (Six-base) MAPSORT of: KIEE6224.seq  Check: 9536  from: 1  to: 810



KIEE6224.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     810 
   Size:        281     529
 
AflIII A'CryG_T

Cuts at:      0     571     810 
   Size:        571     239
 
AleI CACnn'nnGTG

Cuts at:      0     243     810 
   Size:        243     567
 
ApaI G_GGCC'C

Cuts at:      0     452     810 
   Size:        452     358
 
ApoI r'AATT_y

Cuts at:      0      92     712     810 
   Size:         92     620      98

  Fragments arranged by size: 

                 620      98      92
 
BanII G_rGCy'C

Cuts at:      0     452     810 
   Size:        452     358
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     343     810 
   Size:        343     467
 
Bme1580I G_kGCm'C

Cuts at:      0     452     810 
   Size:        452     358
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     788     810 
   Size:        788      22
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     491     810 
   Size:        491     319
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     571     810 
   Size:        571     239
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     398     810 
   Size:        398     412
 
Bsp1286I G_dGCh'C

Cuts at:      0     452     810 
   Size:        452     358
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     410     810 
   Size:        410     400
 
BsrDI GCAATG_nn'

Cuts at:      0      65     770     810 
   Size:         65     705      40

  Fragments arranged by size: 

                 705      65      40
 
BsrGI T'GTAC_A

Cuts at:      0     327     810 
   Size:        327     483
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     212     810 
   Size:        212     598
 
BstEII G'GTnAC_C

Cuts at:      0     230     810 
   Size:        230     580
 
BstYI r'GATC_y

Cuts at:      0     612     810 
   Size:        612     198
 
BtgI C'CryG_G

Cuts at:      0      87     810 
   Size:         87     723
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     491     810 
   Size:        281     210     319

  Fragments arranged by size: 

                 319     281     210
 
EcoO109I rG'GnC_Cy

Cuts at:      0     449     810 
   Size:        449     361
 
EcoRI G'AATT_C

Cuts at:      0      92     810 
   Size:         92     718
 
EcoRV GAT'ATC

Cuts at:      0      38     733     810 
   Size:         38     695      77

  Fragments arranged by size: 

                 695      77      38
 
FspI TGC'GCA

Cuts at:      0     216     810 
   Size:        216     594
 
HindIII A'AGCT_T

Cuts at:      0     682     810 
   Size:        682     128
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     763     810 
   Size:        763      47
 
MslI CAynn'nnrTG

Cuts at:      0     243     603     810 
   Size:        243     360     207

  Fragments arranged by size: 

                 360     243     207
 
NcoI C'CATG_G

Cuts at:      0      87     810 
   Size:         87     723
 
NspI r_CATG'y

Cuts at:      0     575     810 
   Size:        575     235
 
PciI A'CATG_T

Cuts at:      0     571     810 
   Size:        571     239
 
PshAI GACnn'nnGTC

Cuts at:      0     588     810 
   Size:        588     222
 
PspOMI G'GGCC_C

Cuts at:      0     448     810 
   Size:        448     362
 
PstI C_TGCA'G

Cuts at:      0     438     810 
   Size:        438     372
 
SfcI C'TryA_G

Cuts at:      0     137     434     810 
   Size:        137     297     376

  Fragments arranged by size: 

                 376     297     137
 
SmlI C'TyrA_G

Cuts at:      0     550     810 
   Size:        550     260
 
StyI C'CwwG_G

Cuts at:      0      87     810 
   Size:         87     723
 
TatI w'GTAC_w

Cuts at:      0     327     810 
   Size:        327     483
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     ApaI     ApoI    BanII    BciVI Bme1580I
     BmrI     BpmI    BpuEI    BseRI Bsp1286I    BspMI    BsrDI    BsrGI
   BstAPI   BstEII    BstYI     BtgI  Eco57MI EcoO109I    EcoRI    EcoRV
     FspI  HindIII     MmeI     MslI     NcoI     NspI     PciI    PshAI
   PspOMI     PstI     SfcI     SmlI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrFI   BssHII    BssSI    BstBI    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI     FalI     FseI
    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI