Restriction map for
pFN21AE6223
(Linear) (Six-base) MAPSORT of: KIEE6223.seq Check: 1714 from: 1 to: 720
KIEE6223.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 672 720
Size: 672 48
Acc65I G'GTAC_C
Cuts at: 0 251 720
Size: 251 469
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 720
Size: 664 56
AflII C'TTAA_G
Cuts at: 0 120 720
Size: 120 600
AhdI GACnn_n'nnGTC
Cuts at: 0 27 720
Size: 27 693
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 290 720
Size: 290 430
AlwNI CAG_nnn'CTG
Cuts at: 0 264 396 690 720
Size: 264 132 294 30
Fragments arranged by size:
294 264 132 30
AvaI C'yCGr_G
Cuts at: 0 439 720
Size: 439 281
BanI G'GyrC_C
Cuts at: 0 159 251 720
Size: 159 92 469
Fragments arranged by size:
469 159 92
BbsI GAAGACnn'nnnn_
Cuts at: 0 543 720
Size: 543 177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 115 149 720
Size: 115 34 571
Fragments arranged by size:
571 115 34
BfrBI ATG'CAT
Cuts at: 0 525 720
Size: 525 195
BglI GCCn_nnn'nGGC
Cuts at: 0 409 720
Size: 409 311
Bme1580I G_kGCm'C
Cuts at: 0 138 279 720
Size: 138 141 441
Fragments arranged by size:
441 141 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 439 720
Size: 28 411 281
Fragments arranged by size:
411 281 28
Bpu10I CC'TnA_GC
Cuts at: 0 492 720
Size: 492 228
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 720
Size: 136 584
BseYI C'CCAG_C
Cuts at: 0 32 720
Size: 32 688
Bsp1286I G_dGCh'C
Cuts at: 0 138 279 720
Size: 138 141 441
Fragments arranged by size:
441 141 138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 32 272 672 720
Size: 32 240 400 48
Fragments arranged by size:
400 240 48 32
BstBI TT'CG_AA
Cuts at: 0 554 720
Size: 554 166
BstYI r'GATC_y
Cuts at: 0 99 720
Size: 99 621
Bsu36I CC'TnA_GG
Cuts at: 0 257 720
Size: 257 463
BtgI C'CryG_G
Cuts at: 0 530 720
Size: 530 190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 140 720
Size: 140 580
BtsI GCAGTG_nn'
Cuts at: 0 608 720
Size: 608 112
EaeI y'GGCC_r
Cuts at: 0 203 572 707 720
Size: 203 369 135 13
Fragments arranged by size:
369 203 135 13
EarI CTCTTCn'nnn_
Cuts at: 0 622 720
Size: 622 98
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 439 664 720
Size: 28 411 225 56
Fragments arranged by size:
411 225 56 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 154 619 720
Size: 154 465 101
Fragments arranged by size:
465 154 101
EcoO109I rG'GnC_Cy
Cuts at: 0 237 261 612 720
Size: 237 24 351 108
Fragments arranged by size:
351 237 108 24
FspI TGC'GCA
Cuts at: 0 91 720
Size: 91 629
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 96 128 367 399 720
Size: 13 32 51 32 239 32 321
Fragments arranged by size:
321 239 51 32 32 32 13
KpnI G_GTAC'C
Cuts at: 0 255 720
Size: 255 465
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 720
Size: 398 322
MscI TGG'CCA
Cuts at: 0 205 574 709 720
Size: 205 369 135 11
Fragments arranged by size:
369 205 135 11
NsiI A_TGCA'T
Cuts at: 0 527 720
Size: 527 193
PasI CC'CwG_GG
Cuts at: 0 698 720
Size: 698 22
PflMI CCAn_nnn'nTGG
Cuts at: 0 396 720
Size: 396 324
PfoI T'CCnGG_A
Cuts at: 0 620 720
Size: 620 100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 433 465 720
Size: 433 32 255
Fragments arranged by size:
433 255 32
PpuMI rG'GwC_Cy
Cuts at: 0 237 261 612 720
Size: 237 24 351 108
Fragments arranged by size:
351 237 108 24
PspXI vC'TCGA_Gb
Cuts at: 0 439 720
Size: 439 281
PstI C_TGCA'G
Cuts at: 0 269 434 669 720
Size: 269 165 235 51
Fragments arranged by size:
269 235 165 51
SbfI CC_TGCA'GG
Cuts at: 0 269 720
Size: 269 451
SfcI C'TryA_G
Cuts at: 0 265 430 665 720
Size: 265 165 235 55
Fragments arranged by size:
265 235 165 55
SmlI C'TyrA_G
Cuts at: 0 120 151 439 720
Size: 120 31 288 281
Fragments arranged by size:
288 281 120 31
StuI AGG'CCT
Cuts at: 0 466 720
Size: 466 254
StyI C'CwwG_G
Cuts at: 0 650 720
Size: 650 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 361 523 690 720
Size: 361 162 167 30
Fragments arranged by size:
361 167 162 30
XhoI C'TCGA_G
Cuts at: 0 439 720
Size: 439 281
Enzymes that do cut:
AarI Acc65I AcuI AflII AhdI AlfI AlwNI AvaI
BanI BbsI BcgI BfrBI BglI Bme1580I BpmI Bpu10I
BpuEI BseYI Bsp1286I BspMI BstBI BstYI Bsu36I BtgI
BtgZI BtsI EaeI EarI Eco57MI EcoNI EcoO109I FspI
Hin4I KpnI MmeI MscI NsiI PasI PflMI PfoI
PpiI PpuMI PspXI PstI SbfI SfcI SmlI StuI
StyI TaqII XhoI
Enzymes that do not cut:
AatII AccI AclI AfeI AflIII AgeI AleI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbeI BbvCI BciVI BclI BglII BlpI
BmgBI BmrI BmtI BplI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstZ17I ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI PacI PciI PmeI PmlI
PshAI PsiI PspOMI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI