(Linear) (Six-base) MAPSORT of: KIEE6222.seq  Check: 8983  from: 1  to: 690



KIEE6222.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

ApaI G_GGCC'C

Cuts at:      0      19     690 
   Size:         19     671
 
BanII G_rGCy'C

Cuts at:      0      19     690 
   Size:         19     671
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     269     561     690 
   Size:        269     292     129

  Fragments arranged by size: 

                 292     269     129
 
BbvCI CC'TCA_GC

Cuts at:      0      38     690 
   Size:         38     652
 
BglII A'GATC_T

Cuts at:      0     473     690 
   Size:        473     217
 
Bme1580I G_kGCm'C

Cuts at:      0      19     690 
   Size:         19     671
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     620     690 
   Size:        620      70
 
Bpu10I CC'TnA_GC

Cuts at:      0      38     690 
   Size:         38     652
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     465     690 
   Size:        465     225
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     593     623     690 
   Size:        593      30      67

  Fragments arranged by size: 

                 593      67      30
 
BseYI C'CCAG_C

Cuts at:      0     534     690 
   Size:        534     156
 
BsiHKAI G_wGCw'C

Cuts at:      0     491     509     649     690 
   Size:        491      18     140      41

  Fragments arranged by size: 

                 491     140      41      18
 
BsmI GAATG_Cn'

Cuts at:      0     582     690 
   Size:        582     108
 
Bsp1286I G_dGCh'C

Cuts at:      0      19     491     509     649     690 
   Size:         19     472      18     140      41

  Fragments arranged by size: 

                 472     140      41      19      18
 
BspHI T'CATG_A

Cuts at:      0     572     690 
   Size:        572     118
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     275     690 
   Size:        275     415
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     478     690 
   Size:        478     212
 
BstYI r'GATC_y

Cuts at:      0     473     690 
   Size:        473     217
 
BtsI GCAGTG_nn'

Cuts at:      0     215     690 
   Size:        215     475
 
DraIII CAC_nnn'GTG

Cuts at:      0     231     356     690 
   Size:        231     125     334

  Fragments arranged by size: 

                 334     231     125
 
EarI CTCTTCn'nnn_

Cuts at:      0     109     690 
   Size:        109     581
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     412     690 
   Size:        412     278
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     620     690 
   Size:        620      70
 
EcoO109I rG'GnC_Cy

Cuts at:      0      16     690 
   Size:         16     674
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     286     690 
   Size:        286     404
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     229     261     690 
   Size:        229      32     429

  Fragments arranged by size: 

                 429     229      32
 
PspOMI G'GGCC_C

Cuts at:      0      15     690 
   Size:         15     675
 
SexAI A'CCwGG_T

Cuts at:      0     227     690 
   Size:        227     463
 
SfcI C'TryA_G

Cuts at:      0     448     690 
   Size:        448     242
 
StyI C'CwwG_G

Cuts at:      0     314     659     690 
   Size:        314     345      31

  Fragments arranged by size: 

                 345     314      31
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      41     328     393     690 
   Size:         41     287      65     297

  Fragments arranged by size: 

                 297     287      65      41
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     593     625     690 
   Size:        593      32      65

  Fragments arranged by size: 

                 593      65      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     176     398     690 
   Size:        176     222     292

  Fragments arranged by size: 

                 292     222     176
 

 Enzymes that do cut:

     ApaI    BanII     BbsI    BbvCI    BglII Bme1580I     BpmI   Bpu10I
     BsaI    BsaXI    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI
    BstXI    BstYI     BtsI   DraIII     EarI     EciI  Eco57MI EcoO109I
     FalI     PpiI   PspOMI    SexAI     SfcI     StyI    TaqII     TstI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
    BmgBI     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI