Restriction map for
pFN21AE6221
(Linear) (Six-base) MAPSORT of: KIEE6221.seq Check: 8284 from: 1 to: 903
KIEE6221.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AleI CACnn'nnGTG
Cuts at: 0 222 903
Size: 222 681
ApaI G_GGCC'C
Cuts at: 0 94 241 903
Size: 94 147 662
Fragments arranged by size:
662 147 94
ApoI r'AATT_y
Cuts at: 0 655 903
Size: 655 248
AvaI C'yCGr_G
Cuts at: 0 79 162 268 903
Size: 79 83 106 635
Fragments arranged by size:
635 106 83 79
BanI G'GyrC_C
Cuts at: 0 167 903
Size: 167 736
BanII G_rGCy'C
Cuts at: 0 94 241 903
Size: 94 147 662
Fragments arranged by size:
662 147 94
BbeI G_GCGC'C
Cuts at: 0 171 903
Size: 171 732
BbsI GAAGACnn'nnnn_
Cuts at: 0 767 903
Size: 767 136
BbvCI CC'TCA_GC
Cuts at: 0 121 199 903
Size: 121 78 704
Fragments arranged by size:
704 121 78
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 88 99 122 133 903
Size: 88 11 23 11 770
Fragments arranged by size:
770 88 23 11 11
BglII A'GATC_T
Cuts at: 0 582 903
Size: 582 321
Bme1580I G_kGCm'C
Cuts at: 0 94 241 903
Size: 94 147 662
Fragments arranged by size:
662 147 94
BmgBI CAC'GTC
Cuts at: 0 342 903
Size: 342 561
BmrI ACTGGGnnnn_n'
Cuts at: 0 446 903
Size: 446 457
Bpu10I CC'TnA_GC
Cuts at: 0 121 199 903
Size: 121 78 704
Fragments arranged by size:
704 121 78
BsaHI Gr'CG_yC
Cuts at: 0 168 903
Size: 168 735
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 329 903
Size: 329 574
BseYI C'CCAG_C
Cuts at: 0 93 903
Size: 93 810
Bsp1286I G_dGCh'C
Cuts at: 0 94 241 903
Size: 94 147 662
Fragments arranged by size:
662 147 94
BsrDI GCAATG_nn'
Cuts at: 0 501 903
Size: 501 402
BssHII G'CGCG_C
Cuts at: 0 134 903
Size: 134 769
BssSI C'ACGA_G
Cuts at: 0 687 903
Size: 687 216
BstXI CCAn_nnnn'nTGG
Cuts at: 0 224 903
Size: 224 679
BstYI r'GATC_y
Cuts at: 0 582 903
Size: 582 321
BtgI C'CryG_G
Cuts at: 0 223 903
Size: 223 680
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 19 621 903
Size: 19 602 282
Fragments arranged by size:
602 282 19
BtsI GCAGTG_nn'
Cuts at: 0 203 903
Size: 203 700
EaeI y'GGCC_r
Cuts at: 0 48 710 831 903
Size: 48 662 121 72
Fragments arranged by size:
662 121 72 48
EarI CTCTTCn'nnn_
Cuts at: 0 689 903
Size: 689 214
EcoO109I rG'GnC_Cy
Cuts at: 0 90 237 238 903
Size: 90 147 1 665
Fragments arranged by size:
665 147 90 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 480 903
Size: 480 423
HaeII r_GCGC'y
Cuts at: 0 63 171 903
Size: 63 108 732
Fragments arranged by size:
732 108 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 683 715 903
Size: 683 32 188
Fragments arranged by size:
683 188 32
KasI G'GCGC_C
Cuts at: 0 167 903
Size: 167 736
MfeI C'AATT_G
Cuts at: 0 866 903
Size: 866 37
MscI TGG'CCA
Cuts at: 0 50 712 833 903
Size: 50 662 121 70
Fragments arranged by size:
662 121 70 50
MslI CAynn'nnrTG
Cuts at: 0 222 877 903
Size: 222 655 26
Fragments arranged by size:
655 222 26
NarI GG'CG_CC
Cuts at: 0 168 903
Size: 168 735
PfoI T'CCnGG_A
Cuts at: 0 280 903
Size: 280 623
PspOMI G'GGCC_C
Cuts at: 0 90 237 903
Size: 90 147 666
Fragments arranged by size:
666 147 90
PspXI vC'TCGA_Gb
Cuts at: 0 79 903
Size: 79 824
SfoI GGC'GCC
Cuts at: 0 169 903
Size: 169 734
SmaI CCC'GGG
Cuts at: 0 270 903
Size: 270 633
SmlI C'TyrA_G
Cuts at: 0 79 903
Size: 79 824
SrfI GCCC'GGGC
Cuts at: 0 270 903
Size: 270 633
StyI C'CwwG_G
Cuts at: 0 746 903
Size: 746 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 34 903
Size: 34 869
TatI w'GTAC_w
Cuts at: 0 791 903
Size: 791 112
XbaI T'CTAG_A
Cuts at: 0 819 903
Size: 819 84
XhoI C'TCGA_G
Cuts at: 0 79 903
Size: 79 824
XmaI C'CCGG_G
Cuts at: 0 268 903
Size: 268 635
Enzymes that do cut:
AleI ApaI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BglII Bme1580I BmgBI BmrI Bpu10I BsaHI
BseRI BseYI Bsp1286I BsrDI BssHII BssSI BstXI BstYI
BtgI BtgZI BtsI EaeI EarI EcoO109I FalI HaeII
Hin4I KasI MfeI MscI MslI NarI PfoI PspOMI
PspXI SfoI SmaI SmlI SrfI StyI TaqII TatI
XbaI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI
BglI BlpI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BstAPI
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MluI
MmeI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI
SphI SspI StuI SwaI TstI Tth111I XcmI XmnI
ZraI