Restriction map for
pFN21AE6220
(Linear) (Six-base) MAPSORT of: KIEE6220.seq Check: 4844 from: 1 to: 864
KIEE6220.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AccI GT'mk_AC
Cuts at: 0 578 864
Size: 578 286
Acc65I G'GTAC_C
Cuts at: 0 284 864
Size: 284 580
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 864
Size: 82 782
AlwNI CAG_nnn'CTG
Cuts at: 0 242 698 775 864
Size: 242 456 77 89
Fragments arranged by size:
456 242 89 77
ApaI G_GGCC'C
Cuts at: 0 217 315 864
Size: 217 98 549
Fragments arranged by size:
549 217 98
ApoI r'AATT_y
Cuts at: 0 445 465 864
Size: 445 20 399
Fragments arranged by size:
445 399 20
BamHI G'GATC_C
Cuts at: 0 14 864
Size: 14 850
BanI G'GyrC_C
Cuts at: 0 265 284 371 718 864
Size: 265 19 87 347 146
Fragments arranged by size:
347 265 146 87 19
BanII G_rGCy'C
Cuts at: 0 147 174 217 315 864
Size: 147 27 43 98 549
Fragments arranged by size:
549 147 98 43 27
BbeI G_GCGC'C
Cuts at: 0 269 375 864
Size: 269 106 489
Fragments arranged by size:
489 269 106
BbsI GAAGACnn'nnnn_
Cuts at: 0 70 524 864
Size: 70 454 340
Fragments arranged by size:
454 340 70
BfrBI ATG'CAT
Cuts at: 0 733 864
Size: 733 131
Bme1580I G_kGCm'C
Cuts at: 0 217 315 619 864
Size: 217 98 304 245
Fragments arranged by size:
304 245 217 98
BmrI ACTGGGnnnn_n'
Cuts at: 0 125 313 504 607 816 864
Size: 125 188 191 103 209 48
Fragments arranged by size:
209 191 188 125 103 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 469 864
Size: 131 338 395
Fragments arranged by size:
395 338 131
Bpu10I CC'TnA_GC
Cuts at: 0 634 864
Size: 634 230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 294 864
Size: 167 127 570
Fragments arranged by size:
570 167 127
BsaHI Gr'CG_yC
Cuts at: 0 266 372 864
Size: 266 106 492
Fragments arranged by size:
492 266 106
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 556 864
Size: 556 308
BseYI C'CCAG_C
Cuts at: 0 79 453 864
Size: 79 374 411
Fragments arranged by size:
411 374 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 722 864
Size: 722 142
BsiHKAI G_wGCw'C
Cuts at: 0 147 864
Size: 147 717
Bsp1286I G_dGCh'C
Cuts at: 0 147 174 217 315 619 864
Size: 147 27 43 98 304 245
Fragments arranged by size:
304 245 147 98 43 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 412 864
Size: 412 452
BsrBI CCG'CTC
Cuts at: 0 278 401 864
Size: 278 123 463
Fragments arranged by size:
463 278 123
BstEII G'GTnAC_C
Cuts at: 0 529 864
Size: 529 335
BstXI CCAn_nnnn'nTGG
Cuts at: 0 102 796 864
Size: 102 694 68
Fragments arranged by size:
694 102 68
BstYI r'GATC_y
Cuts at: 0 3 14 864
Size: 3 11 850
Fragments arranged by size:
850 11 3
Bsu36I CC'TnA_GG
Cuts at: 0 302 813 864
Size: 302 511 51
Fragments arranged by size:
511 302 51
BtgI C'CryG_G
Cuts at: 0 714 819 864
Size: 714 105 45
Fragments arranged by size:
714 105 45
BtsI GCAGTG_nn'
Cuts at: 0 617 710 776 864
Size: 617 93 66 88
Fragments arranged by size:
617 93 88 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 550 864
Size: 550 314
EcoICRI GAG'CTC
Cuts at: 0 145 864
Size: 145 719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 131 469 864
Size: 82 49 338 395
Fragments arranged by size:
395 338 82 49
EcoNI CCTnn'n_nnAGG
Cuts at: 0 301 571 840 864
Size: 301 270 269 24
Fragments arranged by size:
301 270 269 24
EcoO109I rG'GnC_Cy
Cuts at: 0 49 91 178 214 232 311 312 653
Size: 49 42 87 36 18 79 1 341
Cuts at: 653 864
Size: 211
Fragments arranged by size:
341 211 87 79 49 42 36 18
1
EcoRI G'AATT_C
Cuts at: 0 445 864
Size: 445 419
HaeII r_GCGC'y
Cuts at: 0 269 375 864
Size: 269 106 489
Fragments arranged by size:
489 269 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 737 769 796 828 864
Size: 737 32 27 32 36
Fragments arranged by size:
737 36 32 32 27
KasI G'GCGC_C
Cuts at: 0 265 371 864
Size: 265 106 493
Fragments arranged by size:
493 265 106
KpnI G_GTAC'C
Cuts at: 0 288 864
Size: 288 576
MslI CAynn'nnrTG
Cuts at: 0 794 864
Size: 794 70
MspA1I CmG'CkG
Cuts at: 0 83 591 864
Size: 83 508 273
Fragments arranged by size:
508 273 83
NarI GG'CG_CC
Cuts at: 0 266 372 864
Size: 266 106 492
Fragments arranged by size:
492 266 106
NsiI A_TGCA'T
Cuts at: 0 735 864
Size: 735 129
PasI CC'CwG_GG
Cuts at: 0 95 864
Size: 95 769
PfoI T'CCnGG_A
Cuts at: 0 138 147 864
Size: 138 9 717
Fragments arranged by size:
717 138 9
PpuMI rG'GwC_Cy
Cuts at: 0 91 178 864
Size: 91 87 686
Fragments arranged by size:
686 91 87
PspOMI G'GGCC_C
Cuts at: 0 213 311 864
Size: 213 98 553
Fragments arranged by size:
553 213 98
PstI C_TGCA'G
Cuts at: 0 624 864
Size: 624 240
PvuII CAG'CTG
Cuts at: 0 83 591 864
Size: 83 508 273
Fragments arranged by size:
508 273 83
SacI G_AGCT'C
Cuts at: 0 147 864
Size: 147 717
SfcI C'TryA_G
Cuts at: 0 414 620 864
Size: 414 206 244
Fragments arranged by size:
414 244 206
SfoI GGC'GCC
Cuts at: 0 267 373 864
Size: 267 106 491
Fragments arranged by size:
491 267 106
SmlI C'TyrA_G
Cuts at: 0 182 273 864
Size: 182 91 591
Fragments arranged by size:
591 182 91
StyI C'CwwG_G
Cuts at: 0 218 864
Size: 218 646
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 864
Size: 27 837
Enzymes that do cut:
AccI Acc65I AcuI AlwNI ApaI ApoI BamHI BanI
BanII BbeI BbsI BfrBI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrBI BstEII BstXI BstYI Bsu36I BtgI BtsI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I KasI
KpnI MslI MspA1I NarI NsiI PasI PfoI PpuMI
PspOMI PstI PvuII SacI SfcI SfoI SmlI StyI
TstI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbvCI BcgI BciVI BclI BglI BglII
BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI
BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NspI PacI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI