(Linear) (Six-base) MAPSORT of: KIEE6218.seq  Check: 6361  from: 1  to: 870



KIEE6218.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     667     870 
   Size:        667     203
 
AccI GT'mk_AC

Cuts at:      0      65     870 
   Size:         65     805
 
AlwNI CAG_nnn'CTG

Cuts at:      0     640     832     870 
   Size:        640     192      38

  Fragments arranged by size: 

                 640     192      38
 
ApoI r'AATT_y

Cuts at:      0     250     412     870 
   Size:        250     162     458

  Fragments arranged by size: 

                 458     250     162
 
BamHI G'GATC_C

Cuts at:      0     745     870 
   Size:        745     125
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     771     870 
   Size:        771      99
 
BglII A'GATC_T

Cuts at:      0     785     870 
   Size:        785      85
 
Bme1580I G_kGCm'C

Cuts at:      0     514     870 
   Size:        514     356
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     599     870 
   Size:        599     271
 
BsaWI w'CCGG_w

Cuts at:      0      29     870 
   Size:         29     841
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     112     870 
   Size:        112     758
 
BseYI C'CCAG_C

Cuts at:      0     832     870 
   Size:        832      38
 
BsiHKAI G_wGCw'C

Cuts at:      0      37     870 
   Size:         37     833
 
Bsp1286I G_dGCh'C

Cuts at:      0      37     514     870 
   Size:         37     477     356

  Fragments arranged by size: 

                 477     356      37
 
BspHI T'CATG_A

Cuts at:      0     125     176     870 
   Size:        125      51     694

  Fragments arranged by size: 

                 694     125      51
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     667     870 
   Size:        667     203
 
BsrBI CCG'CTC

Cuts at:      0     505     870 
   Size:        505     365
 
BstYI r'GATC_y

Cuts at:      0     745     785     870 
   Size:        745      40      85

  Fragments arranged by size: 

                 745      85      40
 
BstZ17I GTA'TAC

Cuts at:      0      66     870 
   Size:         66     804
 
Bsu36I CC'TnA_GG

Cuts at:      0       8     208     870 
   Size:          8     200     662

  Fragments arranged by size: 

                 662     200       8
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     602     637     870 
   Size:        602      35     233

  Fragments arranged by size: 

                 602     233      35
 
EaeI y'GGCC_r

Cuts at:      0     149     870 
   Size:        149     721
 
EarI CTCTTCn'nnn_

Cuts at:      0     451     870 
   Size:        451     419
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     105     870 
   Size:        105     765
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     820     870 
   Size:        820      50
 
EcoO109I rG'GnC_Cy

Cuts at:      0     609     870 
   Size:        609     261
 
EcoRI G'AATT_C

Cuts at:      0     250     870 
   Size:        250     620
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     611     643     870 
   Size:        611      32     227

  Fragments arranged by size: 

                 611     227      32
 
HindIII A'AGCT_T

Cuts at:      0     398     870 
   Size:        398     472
 
MfeI C'AATT_G

Cuts at:      0     188     870 
   Size:        188     682
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     557     870 
   Size:        557     313
 
MscI TGG'CCA

Cuts at:      0     151     870 
   Size:        151     719
 
PsiI TTA'TAA

Cuts at:      0     376     870 
   Size:        376     494
 
PstI C_TGCA'G

Cuts at:      0     456     664     870 
   Size:        456     208     206

  Fragments arranged by size: 

                 456     208     206
 
SapI GCTCTTCn'nnn_

Cuts at:      0     451     870 
   Size:        451     419
 
SbfI CC_TGCA'GG

Cuts at:      0     664     870 
   Size:        664     206
 
SexAI A'CCwGG_T

Cuts at:      0     291     870 
   Size:        291     579
 
SfcI C'TryA_G

Cuts at:      0     452     530     660     758     870 
   Size:        452      78     130      98     112

  Fragments arranged by size: 

                 452     130     112      98      78
 
TatI w'GTAC_w

Cuts at:      0      17     305     870 
   Size:         17     288     565

  Fragments arranged by size: 

                 565     288      17
 
XmnI GAAnn'nnTTC

Cuts at:      0     778     870 
   Size:        778      92
 

 Enzymes that do cut:

     AarI     AccI    AlwNI     ApoI    BamHI     BbsI    BglII Bme1580I
     BsaI    BsaWI    BseRI    BseYI  BsiHKAI Bsp1286I    BspHI    BspMI
    BsrBI    BstYI  BstZ17I   Bsu36I    CspCI     EaeI     EarI     EciI
    EcoNI EcoO109I    EcoRI    Hin4I  HindIII     MfeI     MmeI     MscI
     PsiI     PstI     SapI     SbfI    SexAI     SfcI     TatI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EagI
  EcoICRI  Eco57MI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MluI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI