Restriction map for
pFN21AE6214
(Linear) (Six-base) MAPSORT of: KIEE6214.seq Check: 4992 from: 1 to: 813
KIEE6214.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 459 813
Size: 459 354
AfeI AGC'GCT
Cuts at: 0 544 813
Size: 544 269
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 268 813
Size: 268 545
ApoI r'AATT_y
Cuts at: 0 231 813
Size: 231 582
AvaI C'yCGr_G
Cuts at: 0 51 813
Size: 51 762
BamHI G'GATC_C
Cuts at: 0 752 813
Size: 752 61
BanI G'GyrC_C
Cuts at: 0 675 813
Size: 675 138
BanII G_rGCy'C
Cuts at: 0 160 325 706 813
Size: 160 165 381 107
Fragments arranged by size:
381 165 160 107
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 574 608 813
Size: 574 34 205
Fragments arranged by size:
574 205 34
Bme1580I G_kGCm'C
Cuts at: 0 140 813
Size: 140 673
BmgBI CAC'GTC
Cuts at: 0 309 813
Size: 309 504
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 360 559 813
Size: 339 21 199 254
Fragments arranged by size:
339 254 199 21
BsaI GGTCTCn'nnnn_
Cuts at: 0 11 813
Size: 11 802
BsaAI yAC'GTr
Cuts at: 0 462 813
Size: 462 351
BsaHI Gr'CG_yC
Cuts at: 0 75 813
Size: 75 738
BsaWI w'CCGG_w
Cuts at: 0 423 727 813
Size: 423 304 86
Fragments arranged by size:
423 304 86
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 748 813
Size: 748 65
BseYI C'CCAG_C
Cuts at: 0 69 221 365 813
Size: 69 152 144 448
Fragments arranged by size:
448 152 144 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 214 813
Size: 196 18 599
Fragments arranged by size:
599 196 18
BsiEI CG_ry'CG
Cuts at: 0 44 813
Size: 44 769
BsiHKAI G_wGCw'C
Cuts at: 0 325 813
Size: 325 488
Bsp1286I G_dGCh'C
Cuts at: 0 140 160 325 706 813
Size: 140 20 165 381 107
Fragments arranged by size:
381 165 140 107 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 355 459 813
Size: 355 104 354
Fragments arranged by size:
355 354 104
BsrFI r'CCGG_y
Cuts at: 0 550 813
Size: 550 263
BssHII G'CGCG_C
Cuts at: 0 65 634 813
Size: 65 569 179
Fragments arranged by size:
569 179 65
BssSI C'ACGA_G
Cuts at: 0 340 813
Size: 340 473
BstEII G'GTnAC_C
Cuts at: 0 495 813
Size: 495 318
BstXI CCAn_nnnn'nTGG
Cuts at: 0 720 813
Size: 720 93
BstYI r'GATC_y
Cuts at: 0 558 752 813
Size: 558 194 61
Fragments arranged by size:
558 194 61
Bsu36I CC'TnA_GG
Cuts at: 0 502 813
Size: 502 311
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 720 813
Size: 720 93
BtsI GCAGTG_nn'
Cuts at: 0 333 813
Size: 333 480
DraIII CAC_nnn'GTG
Cuts at: 0 408 813
Size: 408 405
EaeI y'GGCC_r
Cuts at: 0 41 563 813
Size: 41 522 250
Fragments arranged by size:
522 250 41
EagI C'GGCC_G
Cuts at: 0 41 813
Size: 41 772
EcoICRI GAG'CTC
Cuts at: 0 323 813
Size: 323 490
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 360 559 813
Size: 339 21 199 254
Fragments arranged by size:
339 254 199 21
EcoO109I rG'GnC_Cy
Cuts at: 0 128 314 813
Size: 128 186 499
Fragments arranged by size:
499 186 128
EcoRI G'AATT_C
Cuts at: 0 231 813
Size: 231 582
FspI TGC'GCA
Cuts at: 0 151 351 813
Size: 151 200 462
Fragments arranged by size:
462 200 151
FspAI rTGC'GCAy
Cuts at: 0 151 813
Size: 151 662
HaeII r_GCGC'y
Cuts at: 0 546 773 813
Size: 546 227 40
Fragments arranged by size:
546 227 40
MfeI C'AATT_G
Cuts at: 0 201 813
Size: 201 612
MscI TGG'CCA
Cuts at: 0 565 813
Size: 565 248
MspA1I CmG'CkG
Cuts at: 0 215 252 300 813
Size: 215 37 48 513
Fragments arranged by size:
513 215 48 37
NotI GC'GGCC_GC
Cuts at: 0 41 813
Size: 41 772
PfoI T'CCnGG_A
Cuts at: 0 124 376 813
Size: 124 252 437
Fragments arranged by size:
437 252 124
PmlI CAC'GTG
Cuts at: 0 462 813
Size: 462 351
PpuMI rG'GwC_Cy
Cuts at: 0 128 813
Size: 128 685
PvuII CAG'CTG
Cuts at: 0 215 300 813
Size: 215 85 513
Fragments arranged by size:
513 215 85
SacI G_AGCT'C
Cuts at: 0 325 813
Size: 325 488
SmaI CCC'GGG
Cuts at: 0 53 813
Size: 53 760
StyI C'CwwG_G
Cuts at: 0 101 813
Size: 101 712
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 135 276 693 813
Size: 135 141 417 120
Fragments arranged by size:
417 141 135 120
XmaI C'CCGG_G
Cuts at: 0 51 813
Size: 51 762
Enzymes that do cut:
AarI AfeI AlfI ApoI AvaI BamHI BanI BanII
BcgI Bme1580I BmgBI BpmI BsaI BsaAI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI
BssHII BssSI BstEII BstXI BstYI Bsu36I BtgZI BtsI
DraIII EaeI EagI EcoICRI Eco57MI EcoO109I EcoRI FspI
FspAI HaeII MfeI MscI MspA1I NotI PfoI PmlI
PpuMI PvuII SacI SmaI StyI TaqII XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI BmrI BmtI BplI Bpu10I
BpuEI BsaBI BsaXI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BstAPI BstBI BstZ17I BtgI ClaI
CspCI DraI DrdI EarI EciI EcoNI EcoRV FalI
FseI Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XcmI XhoI XmnI ZraI