(Linear) (Six-base) MAPSORT of: KIEE6213.seq  Check: 8494  from: 1  to: 603



KIEE6213.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     491     603 
   Size:        491     112
 
AleI CACnn'nnGTG

Cuts at:      0     548     603 
   Size:        548      55
 
AlwNI CAG_nnn'CTG

Cuts at:      0     291     603 
   Size:        291     312
 
ApoI r'AATT_y

Cuts at:      0      25     166     375     603 
   Size:         25     141     209     228

  Fragments arranged by size: 

                 228     209     141      25
 
AvrII C'CTAG_G

Cuts at:      0      52     603 
   Size:         52     551
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     389     603 
   Size:        389     214
 
BbvCI CC'TCA_GC

Cuts at:      0     178     603 
   Size:        178     425
 
Bpu10I CC'TnA_GC

Cuts at:      0     178     603 
   Size:        178     425
 
BsaAI yAC'GTr

Cuts at:      0     270     603 
   Size:        270     333
 
BsaWI w'CCGG_w

Cuts at:      0       6     603 
   Size:          6     597
 
BsmI GAATG_Cn'

Cuts at:      0      78     327     603 
   Size:         78     249     276

  Fragments arranged by size: 

                 276     249      78
 
BspEI T'CCGG_A

Cuts at:      0       6     603 
   Size:          6     597
 
BstYI r'GATC_y

Cuts at:      0      48     603 
   Size:         48     555
 
EarI CTCTTCn'nnn_

Cuts at:      0     361     603 
   Size:        361     242
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     491     603 
   Size:        491     112
 
EcoRI G'AATT_C

Cuts at:      0     166     375     603 
   Size:        166     209     228

  Fragments arranged by size: 

                 228     209     166
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      87     465     603 
   Size:         87     378     138

  Fragments arranged by size: 

                 378     138      87
 
MslI CAynn'nnrTG

Cuts at:      0     548     570     603 
   Size:        548      22      33

  Fragments arranged by size: 

                 548      33      22
 
NspI r_CATG'y

Cuts at:      0     123     603 
   Size:        123     480
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     270     291     603 
   Size:        270      21     312

  Fragments arranged by size: 

                 312     270      21
 
PmlI CAC'GTG

Cuts at:      0     270     603 
   Size:        270     333
 
ScaI AGT'ACT

Cuts at:      0     418     603 
   Size:        418     185
 
SfcI C'TryA_G

Cuts at:      0     316     603 
   Size:        316     287
 
StyI C'CwwG_G

Cuts at:      0      52     603 
   Size:         52     551
 
TatI w'GTAC_w

Cuts at:      0     416     603 
   Size:        416     187
 
XmnI GAAnn'nnTTC

Cuts at:      0     434     603 
   Size:        434     169
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI     ApoI    AvrII     BbsI    BbvCI   Bpu10I
    BsaAI    BsaWI     BsmI    BspEI    BstYI     EarI  Eco57MI    EcoRI
     FalI     MslI     NspI    PflMI     PmlI     ScaI     SfcI     StyI
     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaBI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI