Restriction map for
pFN21AE6212
(Linear) (Six-base) MAPSORT of: KIEE6212.seq Check: 2182 from: 1 to: 714
KIEE6212.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
Acc65I G'GTAC_C
Cuts at: 0 128 714
Size: 128 586
AflIII A'CryG_T
Cuts at: 0 122 140 714
Size: 122 18 574
Fragments arranged by size:
574 122 18
AleI CACnn'nnGTG
Cuts at: 0 556 714
Size: 556 158
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 521 714
Size: 521 193
ApaLI G'TGCA_C
Cuts at: 0 298 693 714
Size: 298 395 21
Fragments arranged by size:
395 298 21
ApoI r'AATT_y
Cuts at: 0 538 714
Size: 538 176
AvaI C'yCGr_G
Cuts at: 0 226 364 714
Size: 226 138 350
Fragments arranged by size:
350 226 138
BanI G'GyrC_C
Cuts at: 0 128 714
Size: 128 586
BanII G_rGCy'C
Cuts at: 0 227 373 381 415 714
Size: 227 146 8 34 299
Fragments arranged by size:
299 227 146 34 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 378 714
Size: 378 336
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 9 43 714
Size: 9 34 671
Fragments arranged by size:
671 34 9
BglII A'GATC_T
Cuts at: 0 95 714
Size: 95 619
Bme1580I G_kGCm'C
Cuts at: 0 302 697 714
Size: 302 395 17
Fragments arranged by size:
395 302 17
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 256 565 714
Size: 76 180 309 149
Fragments arranged by size:
309 180 149 76
BsaAI yAC'GTr
Cuts at: 0 123 168 714
Size: 123 45 546
Fragments arranged by size:
546 123 45
BsaHI Gr'CG_yC
Cuts at: 0 59 641 714
Size: 59 582 73
Fragments arranged by size:
582 73 59
BsaWI w'CCGG_w
Cuts at: 0 472 714
Size: 472 242
BseYI C'CCAG_C
Cuts at: 0 38 147 233 714
Size: 38 109 86 481
Fragments arranged by size:
481 109 86 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 589 714
Size: 580 9 125
Fragments arranged by size:
580 125 9
BsiEI CG_ry'CG
Cuts at: 0 433 714
Size: 433 281
BsiHKAI G_wGCw'C
Cuts at: 0 302 415 697 714
Size: 302 113 282 17
Fragments arranged by size:
302 282 113 17
Bsp1286I G_dGCh'C
Cuts at: 0 227 302 373 381 415 697 714
Size: 227 75 71 8 34 282 17
Fragments arranged by size:
282 227 75 71 34 17 8
BspEI T'CCGG_A
Cuts at: 0 472 714
Size: 472 242
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 275 714
Size: 275 439
BsrBI CCG'CTC
Cuts at: 0 309 617 714
Size: 309 308 97
Fragments arranged by size:
309 308 97
BsrDI GCAATG_nn'
Cuts at: 0 10 662 714
Size: 10 652 52
Fragments arranged by size:
652 52 10
BssHII G'CGCG_C
Cuts at: 0 238 714
Size: 238 476
BssSI C'ACGA_G
Cuts at: 0 286 714
Size: 286 428
BstAPI GCAn_nnn'nTGC
Cuts at: 0 216 567 714
Size: 216 351 147
Fragments arranged by size:
351 216 147
BstBI TT'CG_AA
Cuts at: 0 392 714
Size: 392 322
BstEII G'GTnAC_C
Cuts at: 0 465 480 714
Size: 465 15 234
Fragments arranged by size:
465 234 15
BstYI r'GATC_y
Cuts at: 0 95 714
Size: 95 619
BtgI C'CryG_G
Cuts at: 0 279 584 714
Size: 279 305 130
Fragments arranged by size:
305 279 130
DrdI GACnn_nn'nnGTC
Cuts at: 0 463 714
Size: 463 251
EaeI y'GGCC_r
Cuts at: 0 430 714
Size: 430 284
EagI C'GGCC_G
Cuts at: 0 430 714
Size: 430 284
EcoICRI GAG'CTC
Cuts at: 0 413 714
Size: 413 301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 256 565 714
Size: 76 180 309 149
Fragments arranged by size:
309 180 149 76
EcoRI G'AATT_C
Cuts at: 0 538 714
Size: 538 176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 368 400 714
Size: 368 32 314
Fragments arranged by size:
368 314 32
HincII GTy'rAC
Cuts at: 0 5 714
Size: 5 709
KpnI G_GTAC'C
Cuts at: 0 132 714
Size: 132 582
MluI A'CGCG_T
Cuts at: 0 140 714
Size: 140 574
MslI CAynn'nnrTG
Cuts at: 0 556 714
Size: 556 158
MspA1I CmG'CkG
Cuts at: 0 207 338 586 714
Size: 207 131 248 128
Fragments arranged by size:
248 207 131 128
NdeI CA'TA_TG
Cuts at: 0 86 714
Size: 86 628
PfoI T'CCnGG_A
Cuts at: 0 542 714
Size: 542 172
PmlI CAC'GTG
Cuts at: 0 123 168 714
Size: 123 45 546
Fragments arranged by size:
546 123 45
PstI C_TGCA'G
Cuts at: 0 212 260 497 714
Size: 212 48 237 217
Fragments arranged by size:
237 217 212 48
PvuII CAG'CTG
Cuts at: 0 207 714
Size: 207 507
SacI G_AGCT'C
Cuts at: 0 415 714
Size: 415 299
SacII CC_GC'GG
Cuts at: 0 587 714
Size: 587 127
SfcI C'TryA_G
Cuts at: 0 208 256 493 677 714
Size: 208 48 237 184 37
Fragments arranged by size:
237 208 184 48 37
StyI C'CwwG_G
Cuts at: 0 314 714
Size: 314 400
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 475 714
Size: 475 239
XbaI T'CTAG_A
Cuts at: 0 373 714
Size: 373 341
Enzymes that do cut:
Acc65I AflIII AleI AlfI ApaLI ApoI AvaI BanI
BanII BbsI BcgI BglII Bme1580I BpmI BsaAI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI
BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI DrdI EaeI EagI EcoICRI Eco57MI EcoRI Hin4I
HincII KpnI MluI MslI MspA1I NdeI PfoI PmlI
PstI PvuII SacI SacII SfcI StyI TaqII XbaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AhdI AloI AlwNI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglI
BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaBI BsaXI BseRI BsiWI BsmI BsmBI BspHI BsrFI
BsrGI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII EarI EciI EcoNI EcoO109I EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI MfeI
MmeI MscI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XcmI
XhoI XmaI XmnI ZraI