Restriction map for
pFN21AE6211
(Linear) (Six-base) MAPSORT of: KIEE6211.seq Check: 6626 from: 1 to: 603
KIEE6211.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AatII G_ACGT'C
Cuts at: 0 434 603
Size: 434 169
AfeI AGC'GCT
Cuts at: 0 236 603
Size: 236 367
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 257 289 603
Size: 257 32 314
Fragments arranged by size:
314 257 32
BanII G_rGCy'C
Cuts at: 0 148 603
Size: 148 455
BbvCI CC'TCA_GC
Cuts at: 0 590 603
Size: 590 13
BglI GCCn_nnn'nGGC
Cuts at: 0 92 592 603
Size: 92 500 11
Fragments arranged by size:
500 92 11
BlpI GC'TnA_GC
Cuts at: 0 37 238 603
Size: 37 201 365
Fragments arranged by size:
365 201 37
Bme1580I G_kGCm'C
Cuts at: 0 88 603
Size: 88 515
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 105 603
Size: 105 498
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 603
Size: 532 71
Bpu10I CC'TnA_GC
Cuts at: 0 142 590 603
Size: 142 448 13
Fragments arranged by size:
448 142 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 235 603
Size: 118 117 368
Fragments arranged by size:
368 118 117
BsaAI yAC'GTr
Cuts at: 0 165 228 603
Size: 165 63 375
Fragments arranged by size:
375 165 63
BsaHI Gr'CG_yC
Cuts at: 0 431 476 603
Size: 431 45 127
Fragments arranged by size:
431 127 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 7 37 257 287 603
Size: 7 30 220 30 316
Fragments arranged by size:
316 220 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 412 583 603
Size: 46 366 171 20
Fragments arranged by size:
366 171 46 20
Bsp1286I G_dGCh'C
Cuts at: 0 88 148 603
Size: 88 60 455
Fragments arranged by size:
455 88 60
BspHI T'CATG_A
Cuts at: 0 74 603
Size: 74 529
BsrFI r'CCGG_y
Cuts at: 0 598 603
Size: 598 5
BstAPI GCAn_nnn'nTGC
Cuts at: 0 50 314 603
Size: 50 264 289
Fragments arranged by size:
289 264 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 341 603
Size: 341 262
DraIII CAC_nnn'GTG
Cuts at: 0 50 603
Size: 50 553
EaeI y'GGCC_r
Cuts at: 0 93 152 603
Size: 93 59 451
Fragments arranged by size:
451 93 59
EarI CTCTTCn'nnn_
Cuts at: 0 455 603
Size: 455 148
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 106 603
Size: 106 497
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 603
Size: 532 71
FspI TGC'GCA
Cuts at: 0 541 603
Size: 541 62
HaeII r_GCGC'y
Cuts at: 0 238 603
Size: 238 365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 603
Size: 7 32 564
Fragments arranged by size:
564 32 7
HincII GTy'rAC
Cuts at: 0 435 603
Size: 435 168
MscI TGG'CCA
Cuts at: 0 95 603
Size: 95 508
MspA1I CmG'CkG
Cuts at: 0 278 589 603
Size: 278 311 14
Fragments arranged by size:
311 278 14
NaeI GCC'GGC
Cuts at: 0 600 603
Size: 600 3
NgoMIV G'CCGG_C
Cuts at: 0 598 603
Size: 598 5
PflMI CCAn_nnn'nTGG
Cuts at: 0 165 603
Size: 165 438
PfoI T'CCnGG_A
Cuts at: 0 497 603
Size: 497 106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 257 289 603
Size: 257 32 314
Fragments arranged by size:
314 257 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 55 87 603
Size: 55 32 516
Fragments arranged by size:
516 55 32
PstI C_TGCA'G
Cuts at: 0 376 396 603
Size: 376 20 207
Fragments arranged by size:
376 207 20
SfcI C'TryA_G
Cuts at: 0 372 392 603
Size: 372 20 211
Fragments arranged by size:
372 211 20
SmlI C'TyrA_G
Cuts at: 0 133 250 603
Size: 133 117 353
Fragments arranged by size:
353 133 117
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 11 43 603
Size: 11 32 560
Fragments arranged by size:
560 32 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 603
Size: 162 441
ZraI GAC'GTC
Cuts at: 0 432 603
Size: 432 171
Enzymes that do cut:
AatII AfeI AloI BanII BbvCI BglI BlpI Bme1580I
BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI
Bsp1286I BspHI BsrFI BstAPI BtgZI DraIII EaeI EarI
EciI Eco57MI FspI HaeII Hin4I HincII MscI MspA1I
NaeI NgoMIV PflMI PfoI PpiI PsrI PstI SfcI
SmlI TstI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbsI BcgI BciVI BclI BfrBI BglII BmgBI BmrI
BmtI BsaI BsaBI BsaWI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtsI ClaI CspCI DraI DrdI EagI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI MslI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpuMI PshAI PsiI PspOMI
PspXI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TatI Tth111I XbaI XhoI XmaI XmnI