Restriction map for
pFN21AE6210
(Linear) (Six-base) MAPSORT of: KIEE6210.seq Check: 1790 from: 1 to: 750
KIEE6210.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AatII G_ACGT'C
Cuts at: 0 557 750
Size: 557 193
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 750
Size: 205 545
AfeI AGC'GCT
Cuts at: 0 304 750
Size: 304 446
AleI CACnn'nnGTG
Cuts at: 0 730 750
Size: 730 20
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 154 186 750
Size: 154 32 564
Fragments arranged by size:
564 154 32
ApoI r'AATT_y
Cuts at: 0 151 750
Size: 151 599
AvaI C'yCGr_G
Cuts at: 0 132 362 750
Size: 132 230 388
Fragments arranged by size:
388 230 132
BanI G'GyrC_C
Cuts at: 0 127 621 703 750
Size: 127 494 82 47
Fragments arranged by size:
494 127 82 47
BanII G_rGCy'C
Cuts at: 0 58 97 617 750
Size: 58 39 520 133
Fragments arranged by size:
520 133 58 39
BbeI G_GCGC'C
Cuts at: 0 131 750
Size: 131 619
BbvCI CC'TCA_GC
Cuts at: 0 543 750
Size: 543 207
BclI T'GATC_A
Cuts at: 0 425 750
Size: 425 325
BglI GCCn_nnn'nGGC
Cuts at: 0 79 689 750
Size: 79 610 61
Fragments arranged by size:
610 79 61
Bme1580I G_kGCm'C
Cuts at: 0 542 666 706 727 750
Size: 542 124 40 21 23
Fragments arranged by size:
542 124 40 23 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 588 750
Size: 588 162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 750
Size: 601 149
Bpu10I CC'TnA_GC
Cuts at: 0 52 543 750
Size: 52 491 207
Fragments arranged by size:
491 207 52
BsaI GGTCTCn'nnnn_
Cuts at: 0 643 750
Size: 643 107
BsaBI GATnn'nnATC
Cuts at: 0 430 750
Size: 430 320
BsaHI Gr'CG_yC
Cuts at: 0 128 207 554 750
Size: 128 79 347 196
Fragments arranged by size:
347 196 128 79
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 79 109 750
Size: 79 30 641
Fragments arranged by size:
641 79 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 589 750
Size: 589 161
BseYI C'CCAG_C
Cuts at: 0 372 494 750
Size: 372 122 256
Fragments arranged by size:
372 256 122
BsiHKAI G_wGCw'C
Cuts at: 0 353 578 617 750
Size: 353 225 39 133
Fragments arranged by size:
353 225 133 39
Bsp1286I G_dGCh'C
Cuts at: 0 58 97 353 542 578 617 666 706
Size: 58 39 256 189 36 39 49 40
Cuts at: 706 727 750
Size: 21 23
Fragments arranged by size:
256 189 58 49 40 39 39 36
23 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 536 750
Size: 536 214
BsrFI r'CCGG_y
Cuts at: 0 259 750
Size: 259 491
BssSI C'ACGA_G
Cuts at: 0 697 750
Size: 697 53
BstEII G'GTnAC_C
Cuts at: 0 342 750
Size: 342 408
BstXI CCAn_nnnn'nTGG
Cuts at: 0 684 750
Size: 684 66
Bsu36I CC'TnA_GG
Cuts at: 0 601 750
Size: 601 149
BtgI C'CryG_G
Cuts at: 0 337 644 750
Size: 337 307 106
Fragments arranged by size:
337 307 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 249 750
Size: 249 501
EaeI y'GGCC_r
Cuts at: 0 257 450 674 750
Size: 257 193 224 76
Fragments arranged by size:
257 224 193 76
EcoICRI GAG'CTC
Cuts at: 0 615 750
Size: 615 135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 601 750
Size: 205 396 149
Fragments arranged by size:
396 205 149
EcoO109I rG'GnC_Cy
Cuts at: 0 582 750
Size: 582 168
FspI TGC'GCA
Cuts at: 0 445 750
Size: 445 305
HaeII r_GCGC'y
Cuts at: 0 131 306 750
Size: 131 175 444
Fragments arranged by size:
444 175 131
KasI G'GCGC_C
Cuts at: 0 127 750
Size: 127 623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 750
Size: 332 418
MscI TGG'CCA
Cuts at: 0 676 750
Size: 676 74
MslI CAynn'nnrTG
Cuts at: 0 40 295 328 718 730 750
Size: 40 255 33 390 12 20
Fragments arranged by size:
390 255 40 33 20 12
MspA1I CmG'CkG
Cuts at: 0 168 750
Size: 168 582
NaeI GCC'GGC
Cuts at: 0 261 750
Size: 261 489
NarI GG'CG_CC
Cuts at: 0 128 750
Size: 128 622
NcoI C'CATG_G
Cuts at: 0 644 750
Size: 644 106
NdeI CA'TA_TG
Cuts at: 0 729 750
Size: 729 21
NgoMIV G'CCGG_C
Cuts at: 0 259 750
Size: 259 491
PasI CC'CwG_GG
Cuts at: 0 666 750
Size: 666 84
PshAI GACnn'nnGTC
Cuts at: 0 655 750
Size: 655 95
PvuII CAG'CTG
Cuts at: 0 168 750
Size: 168 582
SacI G_AGCT'C
Cuts at: 0 617 750
Size: 617 133
SfoI GGC'GCC
Cuts at: 0 129 750
Size: 129 621
StyI C'CwwG_G
Cuts at: 0 77 644 750
Size: 77 567 106
Fragments arranged by size:
567 106 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 160 750
Size: 160 590
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 79 111 750
Size: 79 32 639
Fragments arranged by size:
639 79 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 363 651 684 750
Size: 363 288 33 66
Fragments arranged by size:
363 288 66 33
ZraI GAC'GTC
Cuts at: 0 555 750
Size: 555 195
Enzymes that do cut:
AatII AcuI AfeI AleI AloI ApoI AvaI BanI
BanII BbeI BbvCI BclI BglI Bme1580I BmrI BpmI
Bpu10I BsaI BsaBI BsaHI BsaXI BseRI BseYI BsiHKAI
Bsp1286I BspMI BsrFI BssSI BstEII BstXI Bsu36I BtgI
BtgZI EaeI EcoICRI Eco57MI EcoO109I FspI HaeII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV PasI PshAI PvuII SacI SfoI StyI TaqII
TstI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BcgI BciVI BfrBI BglII BlpI
BmgBI BmtI BplI BpuEI BsaAI BsaWI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstYI BstZ17I BtsI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoNI EcoRI
EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XhoI XmaI
XmnI