Restriction map for
pFN21AE6209
(Linear) (Six-base) MAPSORT of: KIEE6209.seq Check: 1217 from: 1 to: 759
KIEE6209.orf
With 182 enzymes: SgfI *
March 16, 2015 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 120 759
Size: 120 639
AccI GT'mk_AC
Cuts at: 0 701 759
Size: 701 58
Acc65I G'GTAC_C
Cuts at: 0 637 759
Size: 637 122
AfeI AGC'GCT
Cuts at: 0 187 759
Size: 187 572
AleI CACnn'nnGTG
Cuts at: 0 709 759
Size: 709 50
ApaLI G'TGCA_C
Cuts at: 0 232 759
Size: 232 527
ApoI r'AATT_y
Cuts at: 0 555 759
Size: 555 204
BanI G'GyrC_C
Cuts at: 0 97 637 759
Size: 97 540 122
Fragments arranged by size:
540 122 97
BanII G_rGCy'C
Cuts at: 0 270 463 759
Size: 270 193 296
Fragments arranged by size:
296 270 193
BbeI G_GCGC'C
Cuts at: 0 101 759
Size: 101 658
BglI GCCn_nnn'nGGC
Cuts at: 0 305 759
Size: 305 454
Bme1580I G_kGCm'C
Cuts at: 0 236 451 759
Size: 236 215 308
Fragments arranged by size:
308 236 215
Bpu10I CC'TnA_GC
Cuts at: 0 45 759
Size: 45 714
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 759
Size: 692 67
BsaHI Gr'CG_yC
Cuts at: 0 98 170 759
Size: 98 72 589
Fragments arranged by size:
589 98 72
BseYI C'CCAG_C
Cuts at: 0 188 252 443 759
Size: 188 64 191 316
Fragments arranged by size:
316 191 188 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 510 651 759
Size: 510 141 108
Fragments arranged by size:
510 141 108
BsiHKAI G_wGCw'C
Cuts at: 0 197 236 270 759
Size: 197 39 34 489
Fragments arranged by size:
489 197 39 34
Bsp1286I G_dGCh'C
Cuts at: 0 197 236 270 451 463 759
Size: 197 39 34 181 12 296
Fragments arranged by size:
296 197 181 39 34 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 120 759
Size: 120 639
BsrDI GCAATG_nn'
Cuts at: 0 609 759
Size: 609 150
BssSI C'ACGA_G
Cuts at: 0 343 456 759
Size: 343 113 303
Fragments arranged by size:
343 303 113
BstYI r'GATC_y
Cuts at: 0 7 29 759
Size: 7 22 730
Fragments arranged by size:
730 22 7
BtgI C'CryG_G
Cuts at: 0 124 377 759
Size: 124 253 382
Fragments arranged by size:
382 253 124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 739 759
Size: 739 20
BtsI GCAGTG_nn'
Cuts at: 0 159 197 207 249 759
Size: 159 38 10 42 510
Fragments arranged by size:
510 159 42 38 10
DraIII CAC_nnn'GTG
Cuts at: 0 728 759
Size: 728 31
DrdI GACnn_nn'nnGTC
Cuts at: 0 697 759
Size: 697 62
EaeI y'GGCC_r
Cuts at: 0 678 759
Size: 678 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 286 759
Size: 286 473
EcoICRI GAG'CTC
Cuts at: 0 268 759
Size: 268 491
HaeII r_GCGC'y
Cuts at: 0 101 189 759
Size: 101 88 570
Fragments arranged by size:
570 101 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 31 759
Size: 31 728
KasI G'GCGC_C
Cuts at: 0 97 759
Size: 97 662
KpnI G_GTAC'C
Cuts at: 0 641 759
Size: 641 118
MslI CAynn'nnrTG
Cuts at: 0 329 343 493 709 759
Size: 329 14 150 216 50
Fragments arranged by size:
329 216 150 50 14
MspA1I CmG'CkG
Cuts at: 0 407 759
Size: 407 352
NarI GG'CG_CC
Cuts at: 0 98 759
Size: 98 661
NcoI C'CATG_G
Cuts at: 0 124 377 759
Size: 124 253 382
Fragments arranged by size:
382 253 124
PasI CC'CwG_GG
Cuts at: 0 75 759
Size: 75 684
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 414 446 759
Size: 414 32 313
Fragments arranged by size:
414 313 32
PstI C_TGCA'G
Cuts at: 0 29 63 759
Size: 29 34 696
Fragments arranged by size:
696 34 29
SacI G_AGCT'C
Cuts at: 0 270 759
Size: 270 489
SfcI C'TryA_G
Cuts at: 0 25 59 759
Size: 25 34 700
Fragments arranged by size:
700 34 25
SfoI GGC'GCC
Cuts at: 0 99 759
Size: 99 660
SmlI C'TyrA_G
Cuts at: 0 707 759
Size: 707 52
StuI AGG'CCT
Cuts at: 0 283 759
Size: 283 476
StyI C'CwwG_G
Cuts at: 0 50 124 377 759
Size: 50 74 253 382
Fragments arranged by size:
382 253 74 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 137 180 759
Size: 137 43 579
Fragments arranged by size:
579 137 43
TatI w'GTAC_w
Cuts at: 0 224 430 759
Size: 224 206 329
Fragments arranged by size:
329 224 206
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 611 643 759
Size: 611 32 116
Fragments arranged by size:
611 116 32
XmnI GAAnn'nnTTC
Cuts at: 0 292 759
Size: 292 467
Enzymes that do cut:
AarI AccI Acc65I AfeI AleI ApaLI ApoI BanI
BanII BbeI BglI Bme1580I Bpu10I BpuEI BsaHI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrDI BssSI BstYI BtgI
BtgZI BtsI DraIII DrdI EaeI EciI EcoICRI HaeII
Hin4I KasI KpnI MslI MspA1I NarI NcoI PasI
PsrI PstI SacI SfcI SfoI SmlI StuI StyI
TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII AclI AcuI AflII AflIII AgeI AhdI AlfI
AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI BpmI BsaI
BsaAI BsaBI BsaWI BsaXI BseRI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI
EagI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI MfeI MluI
MmeI MscI NaeI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI