Restriction map for
pFN21AE6207
(Linear) (Six-base) MAPSORT of: KIEE6207.seq Check: 2333 from: 1 to: 828
KIEE6207.orf
With 182 enzymes: SgfI *
March 16, 2015 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 308 828
Size: 308 520
AccI GT'mk_AC
Cuts at: 0 773 828
Size: 773 55
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 828
Size: 295 533
AflIII A'CryG_T
Cuts at: 0 794 828
Size: 794 34
ApaI G_GGCC'C
Cuts at: 0 452 465 828
Size: 452 13 363
Fragments arranged by size:
452 363 13
AvaI C'yCGr_G
Cuts at: 0 41 281 828
Size: 41 240 547
Fragments arranged by size:
547 240 41
BanI G'GyrC_C
Cuts at: 0 37 145 523 828
Size: 37 108 378 305
Fragments arranged by size:
378 305 108 37
BanII G_rGCy'C
Cuts at: 0 9 48 282 373 452 465 592 828
Size: 9 39 234 91 79 13 127 236
Fragments arranged by size:
236 234 127 91 79 39 13 9
BbeI G_GCGC'C
Cuts at: 0 41 149 828
Size: 41 108 679
Fragments arranged by size:
679 108 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 600 828
Size: 600 228
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 42 76 828
Size: 42 34 752
Fragments arranged by size:
752 42 34
BglI GCCn_nnn'nGGC
Cuts at: 0 94 828
Size: 94 734
BglII A'GATC_T
Cuts at: 0 782 828
Size: 782 46
BlpI GC'TnA_GC
Cuts at: 0 488 828
Size: 488 340
Bme1580I G_kGCm'C
Cuts at: 0 452 465 526 828
Size: 452 13 61 302
Fragments arranged by size:
452 302 61 13
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 707 828
Size: 555 152 121
Fragments arranged by size:
555 152 121
Bpu10I CC'TnA_GC
Cuts at: 0 372 586 828
Size: 372 214 242
Fragments arranged by size:
372 242 214
BsaI GGTCTCn'nnnn_
Cuts at: 0 186 491 703 828
Size: 186 305 212 125
Fragments arranged by size:
305 212 186 125
BsaHI Gr'CG_yC
Cuts at: 0 38 146 828
Size: 38 108 682
Fragments arranged by size:
682 108 38
BseYI C'CCAG_C
Cuts at: 0 518 758 828
Size: 518 240 70
Fragments arranged by size:
518 240 70
BsiEI CG_ry'CG
Cuts at: 0 251 533 828
Size: 251 282 295
Fragments arranged by size:
295 282 251
BsiHKAI G_wGCw'C
Cuts at: 0 9 407 489 828
Size: 9 398 82 339
Fragments arranged by size:
398 339 82 9
Bsp1286I G_dGCh'C
Cuts at: 0 9 48 282 373 407 452 465 489
Size: 9 39 234 91 34 45 13 24
Cuts at: 489 526 592 828
Size: 37 66 236
Fragments arranged by size:
236 234 91 66 45 39 37 34
24 13 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 168 308 574 828
Size: 168 140 266 254
Fragments arranged by size:
266 254 168 140
BssHII G'CGCG_C
Cuts at: 0 155 157 828
Size: 155 2 671
Fragments arranged by size:
671 155 2
BssSI C'ACGA_G
Cuts at: 0 729 828
Size: 729 99
BstEII G'GTnAC_C
Cuts at: 0 624 828
Size: 624 204
BstXI CCAn_nnnn'nTGG
Cuts at: 0 514 828
Size: 514 314
BstYI r'GATC_y
Cuts at: 0 782 828
Size: 782 46
BtgI C'CryG_G
Cuts at: 0 527 828
Size: 527 301
BtsI GCAGTG_nn'
Cuts at: 0 207 255 828
Size: 207 48 573
Fragments arranged by size:
573 207 48
EaeI y'GGCC_r
Cuts at: 0 248 428 530 828
Size: 248 180 102 298
Fragments arranged by size:
298 248 180 102
EagI C'GGCC_G
Cuts at: 0 248 530 828
Size: 248 282 298
Fragments arranged by size:
298 282 248
EarI CTCTTCn'nnn_
Cuts at: 0 193 828
Size: 193 635
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 15 316 828
Size: 15 301 512
Fragments arranged by size:
512 301 15
EcoICRI GAG'CTC
Cuts at: 0 7 828
Size: 7 821
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 555 707 828
Size: 295 260 152 121
Fragments arranged by size:
295 260 152 121
EcoO109I rG'GnC_Cy
Cuts at: 0 376 448 449 462 558 666 691 828
Size: 376 72 1 13 96 108 25 137
Fragments arranged by size:
376 137 108 96 72 25 13 1
HaeII r_GCGC'y
Cuts at: 0 41 103 149 828
Size: 41 62 46 679
Fragments arranged by size:
679 62 46 41
KasI G'GCGC_C
Cuts at: 0 37 145 828
Size: 37 108 683
Fragments arranged by size:
683 108 37
MscI TGG'CCA
Cuts at: 0 430 828
Size: 430 398
MspA1I CmG'CkG
Cuts at: 0 66 482 769 828
Size: 66 416 287 59
Fragments arranged by size:
416 287 66 59
NarI GG'CG_CC
Cuts at: 0 38 146 828
Size: 38 108 682
Fragments arranged by size:
682 108 38
NspI r_CATG'y
Cuts at: 0 798 828
Size: 798 30
PciI A'CATG_T
Cuts at: 0 794 828
Size: 794 34
PfoI T'CCnGG_A
Cuts at: 0 799 828
Size: 799 29
PpuMI rG'GwC_Cy
Cuts at: 0 666 828
Size: 666 162
PspOMI G'GGCC_C
Cuts at: 0 448 461 828
Size: 448 13 367
Fragments arranged by size:
448 367 13
PspXI vC'TCGA_Gb
Cuts at: 0 281 828
Size: 281 547
PstI C_TGCA'G
Cuts at: 0 470 828
Size: 470 358
PvuII CAG'CTG
Cuts at: 0 769 828
Size: 769 59
SacI G_AGCT'C
Cuts at: 0 9 828
Size: 9 819
SapI GCTCTTCn'nnn_
Cuts at: 0 193 828
Size: 193 635
SfcI C'TryA_G
Cuts at: 0 466 828
Size: 466 362
SfoI GGC'GCC
Cuts at: 0 39 147 828
Size: 39 108 681
Fragments arranged by size:
681 108 39
SmaI CCC'GGG
Cuts at: 0 43 828
Size: 43 785
SmlI C'TyrA_G
Cuts at: 0 281 828
Size: 281 547
SrfI GCCC'GGGC
Cuts at: 0 43 828
Size: 43 785
StyI C'CwwG_G
Cuts at: 0 388 717 828
Size: 388 329 111
Fragments arranged by size:
388 329 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 228 793 828
Size: 228 565 35
Fragments arranged by size:
565 228 35
TatI w'GTAC_w
Cuts at: 0 548 828
Size: 548 280
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 682 828
Size: 682 146
XhoI C'TCGA_G
Cuts at: 0 281 828
Size: 281 547
XmaI C'CCGG_G
Cuts at: 0 41 828
Size: 41 787
Enzymes that do cut:
AarI AccI AcuI AflIII ApaI AvaI BanI BanII
BbeI BbsI BcgI BglI BglII BlpI Bme1580I BpmI
Bpu10I BsaI BsaHI BseYI BsiEI BsiHKAI Bsp1286I BspMI
BssHII BssSI BstEII BstXI BstYI BtgI BtsI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII KasI
MscI MspA1I NarI NspI PciI PfoI PpuMI PspOMI
PspXI PstI PvuII SacI SapI SfcI SfoI SmaI
SmlI SrfI StyI TaqII TatI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BmgBI
BmrI BmtI BplI BpuEI BsaAI BsaBI BsaWI BsaXI
BseRI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BstAPI BstBI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MslI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SspI StuI SwaI TstI
Tth111I XbaI XmnI ZraI