Restriction map for
pFN21AE6204
(Linear) (Six-base) MAPSORT of: KIEE6204.seq Check: 9059 from: 1 to: 822
KIEE6204.orf
With 182 enzymes: SgfI *
March 16, 2015 11:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 822
Size: 154 668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 578 822
Size: 578 244
ApaI G_GGCC'C
Cuts at: 0 28 822
Size: 28 794
AvaI C'yCGr_G
Cuts at: 0 21 822
Size: 21 801
BanI G'GyrC_C
Cuts at: 0 99 252 822
Size: 99 153 570
Fragments arranged by size:
570 153 99
BanII G_rGCy'C
Cuts at: 0 28 822
Size: 28 794
BbsI GAAGACnn'nnnn_
Cuts at: 0 296 822
Size: 296 526
BbvCI CC'TCA_GC
Cuts at: 0 148 822
Size: 148 674
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2 36 822
Size: 2 34 786
Fragments arranged by size:
786 34 2
BfrBI ATG'CAT
Cuts at: 0 733 822
Size: 733 89
BlpI GC'TnA_GC
Cuts at: 0 163 822
Size: 163 659
Bme1580I G_kGCm'C
Cuts at: 0 28 104 822
Size: 28 76 718
Fragments arranged by size:
718 76 28
BmgBI CAC'GTC
Cuts at: 0 53 822
Size: 53 769
BmrI ACTGGGnnnn_n'
Cuts at: 0 396 822
Size: 396 426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 822
Size: 374 448
Bpu10I CC'TnA_GC
Cuts at: 0 148 822
Size: 148 674
BsaAI yAC'GTr
Cuts at: 0 785 822
Size: 785 37
BsaBI GATnn'nnATC
Cuts at: 0 605 822
Size: 605 217
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 752 822
Size: 752 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 822
Size: 109 713
BsmI GAATG_Cn'
Cuts at: 0 396 822
Size: 396 426
Bsp1286I G_dGCh'C
Cuts at: 0 28 104 822
Size: 28 76 718
Fragments arranged by size:
718 76 28
BsrBI CCG'CTC
Cuts at: 0 20 167 822
Size: 20 147 655
Fragments arranged by size:
655 147 20
BsrDI GCAATG_nn'
Cuts at: 0 671 822
Size: 671 151
BsrFI r'CCGG_y
Cuts at: 0 182 822
Size: 182 640
BssHII G'CGCG_C
Cuts at: 0 71 822
Size: 71 751
BstAPI GCAn_nnn'nTGC
Cuts at: 0 382 822
Size: 382 440
BtgI C'CryG_G
Cuts at: 0 57 95 200 714 822
Size: 57 38 105 514 108
Fragments arranged by size:
514 108 105 57 38
EaeI y'GGCC_r
Cuts at: 0 47 168 822
Size: 47 121 654
Fragments arranged by size:
654 121 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 374 822
Size: 154 220 448
Fragments arranged by size:
448 220 154
EcoO109I rG'GnC_Cy
Cuts at: 0 588 754 822
Size: 588 166 68
Fragments arranged by size:
588 166 68
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 159 822
Size: 159 663
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 822
Size: 107 715
MslI CAynn'nnrTG
Cuts at: 0 250 728 822
Size: 250 478 94
Fragments arranged by size:
478 250 94
MspA1I CmG'CkG
Cuts at: 0 59 822
Size: 59 763
NaeI GCC'GGC
Cuts at: 0 184 822
Size: 184 638
NcoI C'CATG_G
Cuts at: 0 714 822
Size: 714 108
NdeI CA'TA_TG
Cuts at: 0 378 822
Size: 378 444
NgoMIV G'CCGG_C
Cuts at: 0 182 822
Size: 182 640
NsiI A_TGCA'T
Cuts at: 0 735 822
Size: 735 87
PpuMI rG'GwC_Cy
Cuts at: 0 588 754 822
Size: 588 166 68
Fragments arranged by size:
588 166 68
PspOMI G'GGCC_C
Cuts at: 0 24 822
Size: 24 798
PstI C_TGCA'G
Cuts at: 0 567 822
Size: 567 255
SacII CC_GC'GG
Cuts at: 0 60 822
Size: 60 762
SanDI GG'GwC_CC
Cuts at: 0 588 822
Size: 588 234
SfcI C'TryA_G
Cuts at: 0 563 822
Size: 563 259
StyI C'CwwG_G
Cuts at: 0 83 714 822
Size: 83 631 108
Fragments arranged by size:
631 108 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 358 415 822
Size: 358 57 407
Fragments arranged by size:
407 358 57
XbaI T'CTAG_A
Cuts at: 0 603 822
Size: 603 219
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 804 822
Size: 804 18
XmnI GAAnn'nnTTC
Cuts at: 0 334 822
Size: 334 488
Enzymes that do cut:
AcuI AlfI ApaI AvaI BanI BanII BbsI BbvCI
BcgI BfrBI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaAI BsaBI BseRI BsgI BsmI Bsp1286I BsrBI BsrDI
BsrFI BssHII BstAPI BtgI EaeI Eco57MI EcoO109I FalI
MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI
PpuMI PspOMI PstI SacII SanDI SfcI StyI TaqII
XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI
BglI BglII BmtI BplI BpuEI BsaI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI
BspMI BsrGI BssSI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NarI NheI NotI NruI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XhoI XmaI
ZraI