(Linear) (Six-base) MAPSORT of: KIEE6202.seq  Check: 9476  from: 1  to: 567



KIEE6202.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:27  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     304     567 
   Size:        304     263
 
ApoI r'AATT_y

Cuts at:      0     105     567 
   Size:        105     462
 
BamHI G'GATC_C

Cuts at:      0     356     567 
   Size:        356     211
 
BanI G'GyrC_C

Cuts at:      0     345     567 
   Size:        345     222
 
Bme1580I G_kGCm'C

Cuts at:      0      85     567 
   Size:         85     482
 
BmgBI CAC'GTC

Cuts at:      0     114     567 
   Size:        114     453
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     280     567 
   Size:        280     287
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     452     567 
   Size:        452     115
 
BsaAI yAC'GTr

Cuts at:      0     426     567 
   Size:        426     141
 
BsaWI w'CCGG_w

Cuts at:      0      93     567 
   Size:         93     474
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     567 
   Size:        118     449
 
Bsp1286I G_dGCh'C

Cuts at:      0      85     567 
   Size:         85     482
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     412     567 
   Size:        412     155
 
BssSI C'ACGA_G

Cuts at:      0     388     567 
   Size:        388     179
 
BstYI r'GATC_y

Cuts at:      0     152     356     440     567 
   Size:        152     204      84     127

  Fragments arranged by size: 

                 204     152     127      84
 
EaeI y'GGCC_r

Cuts at:      0     362     567 
   Size:        362     205
 
EarI CTCTTCn'nnn_

Cuts at:      0      46     567 
   Size:         46     521
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     452     567 
   Size:        452     115
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     167     199     567 
   Size:        167      32     368

  Fragments arranged by size: 

                 368     167      32
 
HincII GTy'rAC

Cuts at:      0     291     567 
   Size:        291     276
 
HpaI GTT'AAC

Cuts at:      0     291     567 
   Size:        291     276
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     438     567 
   Size:        438     129
 
MscI TGG'CCA

Cuts at:      0     364     567 
   Size:        364     203
 
PmlI CAC'GTG

Cuts at:      0     426     567 
   Size:        426     141
 
SexAI A'CCwGG_T

Cuts at:      0     542     567 
   Size:        542      25
 

 Enzymes that do cut:

    AlwNI     ApoI    BamHI     BanI Bme1580I    BmgBI     BmrI     BpmI
    BsaAI    BsaWI     BsgI Bsp1286I    BspMI    BssSI    BstYI     EaeI
     EarI  Eco57MI    Hin4I   HincII     HpaI     MmeI     MscI     PmlI
    SexAI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     KasI     KpnI     MfeI     MluI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI