(Linear) (Six-base) MAPSORT of: KIEE6195.seq  Check: 6116  from: 1  to: 402



KIEE6195.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:28  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     259     402 
   Size:        259     143
 
AlwNI CAG_nnn'CTG

Cuts at:      0      82     297     402 
   Size:         82     215     105

  Fragments arranged by size: 

                 215     105      82
 
ApoI r'AATT_y

Cuts at:      0     286     402 
   Size:        286     116
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     306     339     402 
   Size:        306      33      63

  Fragments arranged by size: 

                 306      63      33
 
BbvCI CC'TCA_GC

Cuts at:      0      75     402 
   Size:         75     327
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     303     402 
   Size:        303      99
 
Bpu10I CC'TnA_GC

Cuts at:      0      75     402 
   Size:         75     327
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      91     121     402 
   Size:         91      30     281

  Fragments arranged by size: 

                 281      91      30
 
BsmI GAATG_Cn'

Cuts at:      0     325     402 
   Size:        325      77
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      45     402 
   Size:         45     357
 
BstYI r'GATC_y

Cuts at:      0     293     402 
   Size:        293     109
 
BtgI C'CryG_G

Cuts at:      0      38     350     402 
   Size:         38     312      52

  Fragments arranged by size: 

                 312      52      38
 
BtsI GCAGTG_nn'

Cuts at:      0     233     402 
   Size:        233     169
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     303     402 
   Size:        303      99
 
EcoRI G'AATT_C

Cuts at:      0     286     402 
   Size:        286     116
 
HaeII r_GCGC'y

Cuts at:      0      52     402 
   Size:         52     350
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      58      90     245     277     355     387     402 
   Size:         58      32     155      32      78      32      15

  Fragments arranged by size: 

                 155      78      58      32      32      32      15
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     267     402 
   Size:        267     135
 
MslI CAynn'nnrTG

Cuts at:      0      43     402 
   Size:         43     359
 
MspA1I CmG'CkG

Cuts at:      0     129     402 
   Size:        129     273
 
NcoI C'CATG_G

Cuts at:      0      38     350     402 
   Size:         38     312      52

  Fragments arranged by size: 

                 312      52      38
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     297     402 
   Size:        297     105
 
PvuII CAG'CTG

Cuts at:      0     129     402 
   Size:        129     273
 
StyI C'CwwG_G

Cuts at:      0      38     350     402 
   Size:         38     312      52

  Fragments arranged by size: 

                 312      52      38
 

 Enzymes that do cut:

     AhdI    AlwNI     ApoI     BaeI    BbvCI     BpmI   Bpu10I    BsaXI
     BsmI    BstXI    BstYI     BtgI     BtsI  Eco57MI    EcoRI    HaeII
    Hin4I     MmeI     MslI   MspA1I     NcoI    PflMI    PvuII     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI