(Linear) (Six-base) MAPSORT of: KIEE6194.seq  Check: 1802  from: 1  to: 207



KIEE6194.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:28  ..

BamHI G'GATC_C

Cuts at:      0     170     207 
   Size:        170      37
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      52     207 
   Size:         52     155
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     207 
   Size:        205       2
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     181     207 
   Size:        181      26
 
BsaHI Gr'CG_yC

Cuts at:      0      13     207 
   Size:         13     194
 
BssHII G'CGCG_C

Cuts at:      0      66     207 
   Size:         66     141
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     167     207 
   Size:        167      40
 
BstYI r'GATC_y

Cuts at:      0      74     170     207 
   Size:         74      96      37

  Fragments arranged by size: 

                  96      74      37
 
EaeI y'GGCC_r

Cuts at:      0     119     207 
   Size:        119      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     207 
   Size:        205       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     195     207 
   Size:        195      12
 
HindIII A'AGCT_T

Cuts at:      0     100     207 
   Size:        100     107
 
MspA1I CmG'CkG

Cuts at:      0      18     163     207 
   Size:         18     145      44

  Fragments arranged by size: 

                 145      44      18
 
SfcI C'TryA_G

Cuts at:      0     192     207 
   Size:        192      15
 

 Enzymes that do cut:

    BamHI     BmrI     BpmI     BsaI    BsaHI   BssHII    BstXI    BstYI
     EaeI  Eco57MI    EcoNI  HindIII   MspA1I     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI