(Linear) (Six-base) MAPSORT of: KIEE6193.seq  Check: 7872  from: 1  to: 270



KIEE6193.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:28  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      83     270 
   Size:         83     187
 
ApaI G_GGCC'C

Cuts at:      0      18     270 
   Size:         18     252
 
BamHI G'GATC_C

Cuts at:      0       3     270 
   Size:          3     267
 
BanII G_rGCy'C

Cuts at:      0      18     128     259     270 
   Size:         18     110     131      11

  Fragments arranged by size: 

                 131     110      18      11
 
Bme1580I G_kGCm'C

Cuts at:      0      18     270 
   Size:         18     252
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     270 
   Size:         58     212
 
BsaWI w'CCGG_w

Cuts at:      0      89     270 
   Size:         89     181
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      95     270 
   Size:         95     175
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     270 
   Size:        268       2
 
BsiHKAI G_wGCw'C

Cuts at:      0     128     259     270 
   Size:        128     131      11

  Fragments arranged by size: 

                 131     128      11
 
Bsp1286I G_dGCh'C

Cuts at:      0      18     128     259     270 
   Size:         18     110     131      11

  Fragments arranged by size: 

                 131     110      18      11
 
BsrBI CCG'CTC

Cuts at:      0     189     270 
   Size:        189      81
 
BsrGI T'GTAC_A

Cuts at:      0      62     270 
   Size:         62     208
 
BstYI r'GATC_y

Cuts at:      0       3     270 
   Size:          3     267
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     160     213     270 
   Size:        160      53      57

  Fragments arranged by size: 

                 160      57      53
 
EaeI y'GGCC_r

Cuts at:      0      53     270 
   Size:         53     217
 
EarI CTCTTCn'nnn_

Cuts at:      0     224     235     270 
   Size:        224      11      35

  Fragments arranged by size: 

                 224      35      11
 
EcoICRI GAG'CTC

Cuts at:      0     126     257     270 
   Size:        126     131      13

  Fragments arranged by size: 

                 131     126      13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     270 
   Size:         58     212
 
EcoO109I rG'GnC_Cy

Cuts at:      0      14     270 
   Size:         14     256
 
HincII GTy'rAC

Cuts at:      0     206     270 
   Size:        206      64
 
MspA1I CmG'CkG

Cuts at:      0      24     270 
   Size:         24     246
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      24     270 
   Size:         24     246
 
PspOMI G'GGCC_C

Cuts at:      0      14     270 
   Size:         14     256
 
PvuII CAG'CTG

Cuts at:      0      24     270 
   Size:         24     246
 
SacI G_AGCT'C

Cuts at:      0     128     259     270 
   Size:        128     131      11

  Fragments arranged by size: 

                 131     128      11
 
SapI GCTCTTCn'nnn_

Cuts at:      0     224     270 
   Size:        224      46
 
StyI C'CwwG_G

Cuts at:      0     131     270 
   Size:        131     139
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     170     270 
   Size:        170     100
 
TatI w'GTAC_w

Cuts at:      0      62     270 
   Size:         62     208
 

 Enzymes that do cut:

    AlwNI     ApaI    BamHI    BanII Bme1580I     BpmI    BsaWI    BseRI
     BsgI  BsiHKAI Bsp1286I    BsrBI    BsrGI    BstYI     DrdI     EaeI
     EarI  EcoICRI  Eco57MI EcoO109I   HincII   MspA1I    PflMI   PspOMI
    PvuII     SacI     SapI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseYI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     EagI     EciI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI