(Linear) (Six-base) MAPSORT of: KIEE6192.seq  Check: 1623  from: 1  to: 249



KIEE6192.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     249 
   Size:        205      44
 
AleI CACnn'nnGTG

Cuts at:      0      52     249 
   Size:         52     197
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     182     249 
   Size:        182      67
 
BanII G_rGCy'C

Cuts at:      0      69     249 
   Size:         69     180
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      98     249 
   Size:         98     151
 
BglI GCCn_nnn'nGGC

Cuts at:      0      29     249 
   Size:         29     220
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     219     249 
   Size:        219      30
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     228     249 
   Size:        228      21
 
BseYI C'CCAG_C

Cuts at:      0     105     249 
   Size:        105     144
 
BsiHKAI G_wGCw'C

Cuts at:      0      69     249 
   Size:         69     180
 
Bsp1286I G_dGCh'C

Cuts at:      0      69     249 
   Size:         69     180
 
BspHI T'CATG_A

Cuts at:      0      69     249 
   Size:         69     180
 
BtgI C'CryG_G

Cuts at:      0     221     249 
   Size:        221      28
 
EarI CTCTTCn'nnn_

Cuts at:      0      79     249 
   Size:         79     170
 
EcoICRI GAG'CTC

Cuts at:      0      67     249 
   Size:         67     182
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     228     249 
   Size:        205      23      21

  Fragments arranged by size: 

                 205      23      21
 
MslI CAynn'nnrTG

Cuts at:      0      52      74     249 
   Size:         52      22     175

  Fragments arranged by size: 

                 175      52      22
 
NcoI C'CATG_G

Cuts at:      0     221     249 
   Size:        221      28
 
PfoI T'CCnGG_A

Cuts at:      0     205     249 
   Size:        205      44
 
SacI G_AGCT'C

Cuts at:      0      69     249 
   Size:         69     180
 
SapI GCTCTTCn'nnn_

Cuts at:      0      79     249 
   Size:         79     170
 
StuI AGG'CCT

Cuts at:      0     202     249 
   Size:        202      47
 
StyI C'CwwG_G

Cuts at:      0     221     249 
   Size:        221      28
 

 Enzymes that do cut:

     AcuI     AleI     AlfI    BanII     BbsI     BglI     BplI     BpmI
    BseYI  BsiHKAI Bsp1286I    BspHI     BtgI     EarI  EcoICRI  Eco57MI
     MslI     NcoI     PfoI     SacI     SapI     StuI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI