(Linear) (Six-base) MAPSORT of: KIEE6191.seq  Check: 2971  from: 1  to: 483



KIEE6191.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250     483 
   Size:        250     233
 
ApoI r'AATT_y

Cuts at:      0     477     483 
   Size:        477       6
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     458     483 
   Size:        458      25
 
BclI T'GATC_A

Cuts at:      0     384     483 
   Size:        384      99
 
BglI GCCn_nnn'nGGC

Cuts at:      0     181     483 
   Size:        181     302
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      45     168     483 
   Size:         45     123     315

  Fragments arranged by size: 

                 315     123      45
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     102     483 
   Size:        102     381
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     100     483 
   Size:        100     383
 
BseYI C'CCAG_C

Cuts at:      0       4     483 
   Size:          4     479
 
BsrDI GCAATG_nn'

Cuts at:      0      77     483 
   Size:         77     406
 
EaeI y'GGCC_r

Cuts at:      0     182     483 
   Size:        182     301
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     102     250     483 
   Size:        102     148     233

  Fragments arranged by size: 

                 233     148     102
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     210     483 
   Size:        210     273
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     280     298     312     330     483 
   Size:        280      18      14      18     153

  Fragments arranged by size: 

                 280     153      18      18      14
 
HindIII A'AGCT_T

Cuts at:      0     413     483 
   Size:        413      70
 
MfeI C'AATT_G

Cuts at:      0     470     483 
   Size:        470      13
 
MscI TGG'CCA

Cuts at:      0     184     483 
   Size:        184     299
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      35     483 
   Size:         35     448
 
PfoI T'CCnGG_A

Cuts at:      0      97     483 
   Size:         97     386
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     181     483 
   Size:        181     302
 
StyI C'CwwG_G

Cuts at:      0     185     483 
   Size:        185     298
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     300     483 
   Size:        300     183
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     155     192     483 
   Size:        155      37     291

  Fragments arranged by size: 

                 291     155      37
 

 Enzymes that do cut:

     AcuI     ApoI    BciVI     BclI     BglI     BmrI     BpmI     BsaI
    BseYI    BsrDI     EaeI  Eco57MI     FalI    Hin4I  HindIII     MfeI
     MscI    PflMI     PfoI     SfiI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MluI     MmeI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI