(Linear) (Six-base) MAPSORT of: KIEE6185.seq  Check: 9978  from: 1  to: 492



KIEE6185.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:28  ..

AccI GT'mk_AC

Cuts at:      0      27     492 
   Size:         27     465
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       6     492 
   Size:          6     486
 
ApoI r'AATT_y

Cuts at:      0     365     492 
   Size:        365     127
 
AvaI C'yCGr_G

Cuts at:      0      95     492 
   Size:         95     397
 
BanII G_rGCy'C

Cuts at:      0     233     442     492 
   Size:        233     209      50

  Fragments arranged by size: 

                 233     209      50
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     413     492 
   Size:        413      79
 
BglI GCCn_nnn'nGGC

Cuts at:      0     446     492 
   Size:        446      46
 
BsaAI yAC'GTr

Cuts at:      0     261     492 
   Size:        261     231
 
BsiHKAI G_wGCw'C

Cuts at:      0     233     492 
   Size:        233     259
 
Bsp1286I G_dGCh'C

Cuts at:      0     233     442     492 
   Size:        233     209      50

  Fragments arranged by size: 

                 233     209      50
 
BsrDI GCAATG_nn'

Cuts at:      0     477     492 
   Size:        477      15
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     455     492 
   Size:        455      37
 
BtgI C'CryG_G

Cuts at:      0     123     492 
   Size:        123     369
 
BtsI GCAGTG_nn'

Cuts at:      0     465     492 
   Size:        465      27
 
EarI CTCTTCn'nnn_

Cuts at:      0      25     492 
   Size:         25     467
 
EcoICRI GAG'CTC

Cuts at:      0     231     492 
   Size:        231     261
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       6     492 
   Size:          6     486
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     237     269     454     486     492 
   Size:        237      32     185      32       6

  Fragments arranged by size: 

                 237     185      32      32       6
 
HincII GTy'rAC

Cuts at:      0      28     150     492 
   Size:         28     122     342

  Fragments arranged by size: 

                 342     122      28
 
PfoI T'CCnGG_A

Cuts at:      0     338     492 
   Size:        338     154
 
PsiI TTA'TAA

Cuts at:      0     176     352     492 
   Size:        176     176     140

  Fragments arranged by size: 

                 176     176     140
 
SacI G_AGCT'C

Cuts at:      0     233     492 
   Size:        233     259
 
SalI G'TCGA_C

Cuts at:      0      26     492 
   Size:         26     466
 
SapI GCTCTTCn'nnn_

Cuts at:      0      25     492 
   Size:         25     467
 
SfcI C'TryA_G

Cuts at:      0      46     492 
   Size:         46     446
 
SmaI CCC'GGG

Cuts at:      0      97     492 
   Size:         97     395
 
SspI AAT'ATT

Cuts at:      0     280     492 
   Size:        280     212
 
XmaI C'CCGG_G

Cuts at:      0      95     492 
   Size:         95     397
 
XmnI GAAnn'nnTTC

Cuts at:      0     104     492 
   Size:        104     388
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     AvaI    BanII     BbsI     BglI    BsaAI
  BsiHKAI Bsp1286I    BsrDI   BstAPI     BtgI     BtsI     EarI  EcoICRI
  Eco57MI    Hin4I   HincII     PfoI     PsiI     SacI     SalI     SapI
     SfcI     SmaI     SspI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     ZraI