(Linear) (Six-base) MAPSORT of: KIEE6181.seq  Check: 6524  from: 1  to: 375



KIEE6181.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     375 
   Size:        338      37
 
AfeI AGC'GCT

Cuts at:      0     116     375 
   Size:        116     259
 
ApaI G_GGCC'C

Cuts at:      0       7     183     375 
   Size:          7     176     192

  Fragments arranged by size: 

                 192     176       7
 
ApoI r'AATT_y

Cuts at:      0     316     328     375 
   Size:        316      12      47

  Fragments arranged by size: 

                 316      47      12
 
AvaI C'yCGr_G

Cuts at:      0       6      27     182     375 
   Size:          6      21     155     193

  Fragments arranged by size: 

                 193     155      21       6
 
BanII G_rGCy'C

Cuts at:      0       7     183     375 
   Size:          7     176     192

  Fragments arranged by size: 

                 192     176       7
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      52      86     375 
   Size:         52      34     289

  Fragments arranged by size: 

                 289      52      34
 
BlpI GC'TnA_GC

Cuts at:      0     118     375 
   Size:        118     257
 
Bme1580I G_kGCm'C

Cuts at:      0       7     183     375 
   Size:          7     176     192

  Fragments arranged by size: 

                 192     176       7
 
BseYI C'CCAG_C

Cuts at:      0     173     375 
   Size:        173     202
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     183     375 
   Size:          7     176     192

  Fragments arranged by size: 

                 192     176       7
 
BstBI TT'CG_AA

Cuts at:      0     257     375 
   Size:        257     118
 
BtgI C'CryG_G

Cuts at:      0      33     375 
   Size:         33     342
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     111     375 
   Size:        111     264
 
EarI CTCTTCn'nnn_

Cuts at:      0     297     375 
   Size:        297      78
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     375 
   Size:        338      37
 
EcoO109I rG'GnC_Cy

Cuts at:      0      13     179     375 
   Size:         13     166     196

  Fragments arranged by size: 

                 196     166      13
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     269     375 
   Size:        269     106
 
HaeII r_GCGC'y

Cuts at:      0     118     375 
   Size:        118     257
 
MspA1I CmG'CkG

Cuts at:      0      35     375 
   Size:         35     340
 
PpuMI rG'GwC_Cy

Cuts at:      0      13     375 
   Size:         13     362
 
PspOMI G'GGCC_C

Cuts at:      0       3     179     375 
   Size:          3     176     196

  Fragments arranged by size: 

                 196     176       3
 
SacII CC_GC'GG

Cuts at:      0      36     375 
   Size:         36     339
 
ScaI AGT'ACT

Cuts at:      0     193     375 
   Size:        193     182
 
SfcI C'TryA_G

Cuts at:      0     110     375 
   Size:        110     265
 
SmaI CCC'GGG

Cuts at:      0       8     184     375 
   Size:          8     176     191

  Fragments arranged by size: 

                 191     176       8
 
SspI AAT'ATT

Cuts at:      0     289     375 
   Size:        289      86
 
TatI w'GTAC_w

Cuts at:      0     191     375 
   Size:        191     184
 
XmaI C'CCGG_G

Cuts at:      0       6     182     375 
   Size:          6     176     193

  Fragments arranged by size: 

                 193     176       6
 

 Enzymes that do cut:

     AcuI     AfeI     ApaI     ApoI     AvaI    BanII     BcgI     BlpI
 Bme1580I    BseYI Bsp1286I    BstBI     BtgI    BtgZI     EarI  Eco57MI
 EcoO109I     FalI    HaeII   MspA1I    PpuMI   PspOMI    SacII     ScaI
     SfcI     SmaI     SspI     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI